First Report of <i>Meloidogyne inornata</i> on <i>Smallanthus sonchifolius</i> in Brazil

Camara, Guilherme Resende; Carvalho, A. H. O.; Teixeira, Ariany das Graças; Ferreira, Michael Luiz; Oliveira, Fábio Luiz de; Moraes, Willian Bucker; Alves, Fábio Ramos

doi:10.1094/pdis-08-19-1739-pdn

Cited by 3 publications

(2 citation statements)

References 3 publications

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“…Nevertheless, the esterase phenotype assessment has limitations, as it requires young females and a gel comparative analysis of the obtained patterns for the different species. The first band of the esterase phenotype of M. luci is located at the same level of the first band of M. javanica (reference isolate), whereas the first bands of M. ethiopica and M. inornata are located above, making it almost impossible to distinguish them when they are not in the same gel [ 14 , 15 , 17 , 27 , 30 , 44 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

Species-Specific Molecular Detection of the Root Knot Nematode Meloidogyne luci

Maleita

Cardoso

Rusinque

et al. 2021

Biology

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Meloidogyne luci has been identified in various countries around the world parasitizing economically important crops and, due to its potential to cause serious damage to agriculture, was included in the European and Mediterranean Plant Protection Organization Alert List in 2017. This species shares morphological and molecular similarities with M. ethiopica and M. inornata, and a M. ethiopica group was established. Although specific primers for DNA amplification of species belonging to the M. ethiopica group have been developed previously, primers were not species-specific and molecular markers for the specific detection of M. luci are still needed. The objective of this study was to develop a SCAR marker, for detection of M. luci and discrimination from other Meloidogyne spp., based on the intraspecific variability found in RAPD markers. RAPD screening of M. luci and M. ethiopica genome was used for the identification of a specific amplification product on M. luci, which was cloned, sequenced and converted into a SCAR marker. The specificity of the designed primers (Mlf/r) was tested and produced a fragment (771 bp) for all nine M. luci isolates with no amplification for the other nine Meloidogyne spp., including M. ethiopica and M. inornata. Additionally, the proper amplification of the M. luci SCAR-marker was also successful with DNA from galls of M. luci infected tomato roots. The results obtained in this study reveal that the specific molecular detection of M. luci was achieved and the developed methodology can be used for routine diagnosis purposes, which are essential to monitor M. luci distribution and spread, in order to implement future effective and integrated nematode pest management programs.

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Section: Discussionmentioning

confidence: 99%

Species-Specific Molecular Detection of the Root Knot Nematode Meloidogyne luci

Maleita

Cardoso

Rusinque

et al. 2021

Biology

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“…H. Rob., common bean Phaseolus vulgaris L. and Anthurium andreanum Linden ex André (Whitehead, 1968;Carneiro et al, 2008;Machado et al, 2013;Camara et al, 2020). This nematode, reported by Camara et al (2020) as the cause of damage in commercial yacon production in Brazil, also has potential to cause losses in common bean crops (Dadazio et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Potato (Solanum tuberosum) - a new host for the root-knot nematode Meloidogyne inornata

Stare

Susič

STAROVIČ

et al. 2022

Phytopathol. Mediterr.

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The tropical root-knot nematode (RKN) Meloidogyne inornata infects soybean, tobacco, yacon, common bean and Anthurium andreanum. This species was reported as the cause of damage in commercial yacon production in Brazil, and has potential to cause losses to common bean crops. Potato (Solanum tuberosum L.) cv. Désirée infected with M. inornata exhibited typical RKN galls on roots and tuber surfaces, similar to those caused by the two quarantine pathogens M. chitwoodi and M. fallax, and the non-quarantine M. luci. This study has indicated that M. inornata has considerable potential to cause severe damage to potato tubers. The potential spread of this pathogen into new areas should be assessed, as it can damage potato tubers and could be a problem for economically important crops. Ensuring pest-free seed potatoes is important to prevent dissemination and establishment of M. inornata in uninfested areas. Phytosanitary measures and monitoring programmes developed to prevent spread of this pest in Europe may be warranted.

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Meloidogyne inornata

2021

CABI Compendium

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First Report of Meloidogyne inornata on Smallanthus sonchifolius in Brazil

Cited by 3 publications

References 3 publications

Species-Specific Molecular Detection of the Root Knot Nematode Meloidogyne luci

Species-Specific Molecular Detection of the Root Knot Nematode Meloidogyne luci

Potato (Solanum tuberosum) - a new host for the root-knot nematode Meloidogyne inornata

Meloidogyne inornata

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