First Report of <i>Plum pox virus</i> Strain D Isolate in Peach (<i>Prunus persica</i>) in Korea

Jeong-hak, Oh，; Park, C Y; Lee, H K; Yeom, Y A; Lim, Seungmo; Moon, Jae Sun; Lee, S H

doi:10.1094/pdis-07-16-0979-pdn

Cited by 11 publications

(6 citation statements)

References 3 publications

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“…In contrast, the Korean isolate was closely related to Eastern European isolates (Figure ). This suggests that PPV‐D in Korea (Oh et al , ) has a different origin from the PPV‐D populations in Japan, even though these two Asian countries are geographically close.…”

Section: Discussionmentioning

confidence: 99%

“…Both Figure 2). This suggests that PPV-D in Korea (Oh et al, 2017) has a different origin from the PPV-D populations in Japan, even though these two Asian countries are geographically close.…”

Section: Origin and Dispersal Routes Of Ppv-d In Japanmentioning

confidence: 98%

“…PPV‐Dideron (D), the most widely distributed strain, is widespread throughout Europe and has also been detected in South and North America, Africa, and Asia (EPPO, ; Chirkov et al , ; Oh et al , ), including Japan (Maejima et al , ; Maejima et al , ). In this study, to investigate intra‐strain biological variation in PPV‐D, we performed molecular epidemiological analysis based mainly on virus samples collected in Japan.…”

Section: Introductionmentioning

confidence: 99%

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Intra‐strain biological and epidemiological characterization of plum pox virus

Maejima

Hashimoto

Hagiwara‐Komoda

et al. 2020

Molecular Plant Pathology

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Plum pox virus (PPV) is one of the most important plant viruses causing serious economic losses. Thus far, strain typing based on the definition of 10 monophyletic strains with partially differentiable biological properties has been the sole approach used for epidemiological characterization of PPV. However, elucidating the genetic determinants underlying intra‐strain biological variation among populations or isolates remains a relevant but unexamined aspect of the epidemiology of the virus. In this study, based on complete nucleotide sequence information of 210 Japanese and 47 non‐Japanese isolates of the PPV‐Dideron (D) strain, we identified five positively selected sites in the PPV‐D genome. Among them, molecular studies showed that amino acid substitutions at position 2,635 in viral replicase correlate with viral titre and competitiveness at the systemic level, suggesting that amino acid position 2,635 is involved in aphid transmission efficiency and symptom severity. Estimation of ancestral genome sequences indicated that substitutions at amino acid position 2,635 were reversible and peculiar to one of two genetically distinct PPV‐D populations in Japan. The reversible amino acid evolution probably contributes to the dissemination of the virus population. This study provides the first genomic insight into the evolutionary epidemiology of PPV based on intra‐strain biological variation ascribed to positive selection.

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Section: Discussionmentioning

confidence: 99%

Section: Origin and Dispersal Routes Of Ppv-d In Japanmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Intra‐strain biological and epidemiological characterization of plum pox virus

Maejima

Hashimoto

Hagiwara‐Komoda

et al. 2020

Molecular Plant Pathology

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“…In particular, infection of PPV in P. domestica has been intensively reported in diverse countries ( García et al, 2014 ). Recently, PPV has been reported in different stone fruits in Korea ( Oh et al, 2017 ). Fortunately, we did not identify PPV in our examined plum trees.…”

Section: Discussionmentioning

confidence: 99%

Identification of viruses infecting six plum cultivars in Korea by RNA-sequencing

Choi

Lian

et al. 2020

PeerJ

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Background Plums are a kind of stone fruit, a category that includes peaches, cherries, apricots, and almonds. In Korea, Japanese plum trees are usually cultivated as they best suit the climate. To date, there have been few studies in Korea on viruses infecting plum trees compared to those infecting peach trees. Methods To identify viruses and viroids infecting plum trees, we collected leaf samples from six different plum cultivars and subjected them to RNA-sequencing (RNA-seq). Six different plum transcriptomes were de novo assembled using the Trinity assembler followed by BLAST searching against a viral reference database. Results We identified hop stunt viroid (HSVd) and six viruses, including apple chlorotic leaf spot virus (ACLSV), little cherry virus-1 (LChV-1), peach virus D (PeVD), peach leaf pitting-associated virus (PLPaV), plum bark necrosis stem pitting-associated virus (PBNSPaV), and prunus necrotic ringspot virus (PNRSV), from six plum cultivars by RNA-seq. RT-PCR confirmed the infection of HSVd and three viruses—ACLSV, PBNSPaV, and PNRSV—in plum trees. However, RT-PCR demonstrated that plum trees in this study were not infected by LChV-1, PeVD, or PLPaV. It is likely that the three viruses LChV-1, PeVD, and PLPaV as identified by RNA-seq were contaminants from other peach libraries caused by index misassignment, which suggests that careful confirmation by other methods should be carried out in next-generation sequencing (NGS)-based virus identification. Taken together, we identified a viroid and three viruses infecting plum trees in Korea.

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“…orchards, since PPV was first detected from peach in Korea, 2015. During 2016-2017, samples were collected from 30,333 trees in 1,985 orchards of stone fruits in 8 provinces and 4 cities, Korea and tested by RT-PCR using specific PPV primer set. As a result, 21 trees including peach (9 trees), Japanese apricot (4 trees), plum (1 tree), apricot (7 trees) in 10 orchards were infected and controlled by eradication program.…”

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confidence: 99%

Occurrence and Eradication of Plum Pox Virus on Ornamentals in Korea, 2016-2017

Kim¹,

Kim²,

Kwak³

et al. 2019

Res. Plant Dis

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Plum pox virus (PPV) is a significant viral disease in Prunus spp. worldwide. A nationwide survey was started in Prunus spp. orchards, since PPV was first detected from peach in Korea, 2015. During 2016-2017, samples were collected from 30,333 trees in 1,985 orchards of stone fruits in 8 provinces and 4 cities, Korea and tested by RT-PCR using specific PPV primer set. As a result, 21 trees including peach (9 trees), Japanese apricot (4 trees), plum (1 tree), apricot (7 trees) in 10 orchards were infected and controlled by eradication program. Amplicons of the expected size (547 bp) were obtained from total RNA of seven peach trees in 2016, and directly sequenced. BLAST analysis revealed the highest nucleotide (NT) identity (99%) with a PPV D isolates (LC331298, LT600782) in Genbank. The seven isolates from shared nt sequence identities of 98 to 100% with one another. Phylogenetic analysis showed the isolates in peach clustered closely with the PPV-D isolates from Korea, Japan, USA, and Canada. This is, to our knowledge, the first report of the presence of PPV in Prunus spp. orchards in Korea, 2016-2017, we hope that our results and efforts will contribute to effective measures for eradication of PPV.

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First Report of Plum pox virus Strain D Isolate in Peach (Prunus persica) in Korea

Cited by 11 publications

References 3 publications

Intra‐strain biological and epidemiological characterization of plum pox virus

Intra‐strain biological and epidemiological characterization of plum pox virus

Identification of viruses infecting six plum cultivars in Korea by RNA-sequencing

Occurrence and Eradication of Plum Pox Virus on Ornamentals in Korea, 2016-2017

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