First Report of <i>Pratylenchus zeae</i> on Upland Rice from Kwale County, Kenya

Pili, Njira Njira; Kyndt, Tina; Gheysen, Godelieve; Janssen, Toon; Couvreur, Marjolein; Bert, Wim; Mibey, R. K.

doi:10.1094/pdis-07-15-0743-pdn

Cited by 7 publications

(3 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It causes significant yield losses in rice even at low population densities (Prot and Savary 1993) by reducing grain yield, shoot dry weight and root fresh weight (Prasad and Rao 1988). Pratylenchus zeae has been observed in rainfed upland rice fields in Kwale County, Kenya (Pili et al 2016). This nematode along with Meloidogyne poses a serious threat to rainfed upland rice cultivation in Kenya.…”

Section: Discussionmentioning

confidence: 99%

Distribution of parasitic nematodes in Kenyan rice fields and their relation to edaphic factors, rainfall and temperature

Namu

Karuri

Alakonya

et al. 2017

Trop. plant pathol.

View full text Add to dashboard Cite

Rice is the third most important crop in Kenya after maize and wheat. Plant parasitic nematodes (PPN) are a major rice production constraint. The objective of this study was to determine the distribution and abundance of rice PPN in Kenya and their association with environmental variables. Soil and plant samples were collected from rainfed upland and irrigated lowland rice fields in Kirogo, Thiba, Nyangati, Ombeyi, Kombura, Kakola and Mwambe Sub-locations. Twenty-two PPN genera were identified across all Sublocations with the highest (20) number of nematode genera recorded in rainfed upland rice fields. The nematode genera Scutellonema, Hoplolaimus, Ditylenchus, Hemicycliophora, Heterodera, Paralongidorus and Tylenchus were not recorded in the irrigated lowland rice fields while Criconemella and Tylenchorhynchus were not observed in rainfed upland fields. Canonical correspondence analysis of the relationship between soil properties and nematode abundance revealed a significant (P < 0.05) relationship between PPN abundance and potassium, sodium, sand and clay. The relationship between rainfall, minimum temperature and nematode genera was significant (P < 0.05) while maximum temperature was not significant (P = 0.928). Our results provide critical information for designing nematode management strategies in Kenyan rice fields.

show abstract

Section: Discussionmentioning

confidence: 99%

Distribution of parasitic nematodes in Kenyan rice fields and their relation to edaphic factors, rainfall and temperature

Namu

Karuri

Alakonya

et al. 2017

Trop. plant pathol.

View full text Add to dashboard Cite

show abstract

“…The newly obtained D2-D3 of 28S rRNA, ITS of rRNA, coxI and hsp90 gene sequences were aligned with corresponding published gene sequences (Duncan et al, 1999;Subbotin et al, 2008;De Luca et al, 2010, 2011Palomares-Rius et al, 2010;Majd Taheri et al, 2013;Wang et al, 2015;Pili et al, 2016, and others) using ClustalX 1.83 (Thompson et al, 1997) with default parameters (gap opening 15.0 and gap extension 6.66) for protein coding genes or modified parameters (5.0 and 3.0) for rRNA genes. The alignments were analysed with Bayesian inference (BI) using MrBayes 3.1.2 (Huelsenbeck & Ronquist, 2001) under the GTR + I + G model.…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Characterisation of amphimictic and parthenogenetic populations of Pratylenchus bolivianus Corbett, 1983 (Nematoda: Pratylenchidae) and their phylogenetic relationships with closely related species

Troccoli

Subbotin

Chitambar

et al. 2016

Nematol

View full text Add to dashboard Cite

Amphimictic populations of root-lesion nematodes with numerous males and females having three lip annuli, a functional spermatheca and non-areolated lateral field occur on sword fern (Nephrolepis exaltata) in Florida. Identified for decades asPratylenchus penetrans, they appeared to be a morphologically separated species on the basis of a longer stylet (17.8-18.3 μm) thanP. penetrans(15-17 μm) and different lip pattern inen faceview (rectangularvsdumb-bell inP. penetrans). Morphologically similar amphimictic root-lesion nematodes have also been detected on flax lily in Costa Rica. Subsequent morphological observations indicated that these amphimictic root-lesion nematodes from fern and flax lily are closely related to the parthenogenetic speciesP. bolivianus, which has areolated lateral fields. In spite of the reproductive and morphological dissimilarities between these populations, their separation into different species was not supported by the results of molecular analyses of their DNA sequences. The populations used in these analyses included those that are amphimictic from Florida and Costa Rica and others that are parthenogenetic from the type locality in Bolivia, and geographically distant localities in Chile, China, Colombia and Europe. Phylogenetic analyses of the ITS and D2-D3 expansion segments of the 28S rRNA gene indicated that they belong to the same species,P. bolivianus, which consists of two morphotypes,P. bolivianus(am) amphimictic andP. bolivianus(pm) parthenogenetic, herein described and illustrated. Contradictory results were obtained by the analyses using a portion of thehsp90gene. The phylogenetic study, which included sequences of other root-lesion nematodes, a topotype and geographical distant populations ofP. zeae, revealed thatP. bolivianusandP. zeaeformed highly supported clades in the majority consensus trees. PCR with species-specific primers for rapid diagnostics ofP. bolivianusandP. zeaewere developed and tested.

show abstract

“…Yield decreases have been reported despite increased application of nitrogen fertilizers [ 4 ]. There are several biotic constraints in Kenyan rice production systems such as viruses, fungi [ [5] , [6] , [7] ] and plant-parasitic nematodes (PPN) [ 8 ]. In addition to these constraints, irrigated areas that account for 80% of rice production in Kenya, pose additional stress such as high salinity and low fertility.…”

Section: Introductionmentioning

confidence: 99%