First report of <i>Radish leaf curl virus</i> infecting okra in India

Kumar, Jitendra; Kumar, Abhinav; Singh, Sudhir P.; Roy, Joy; Lalit, Adarsh; Parmar, D. L.; Sharma, Neetu; Tuli, Rakesh

doi:10.5197/j.2044-0588.2012.025.009

Cited by 15 publications

(3 citation statements)

References 4 publications

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“…Although alphasatellites are capable of autonomous replication in permissible plant cells, they depend on helper virus-encoded proteins for movement within and between plants (8). The two alphasatellites were maintained in wheat by WDIV as efficiently as by the begomoviruses (CLCuBuV and CyTLCuV) in tobacco and are thus typical of the earlier described satellites (38,46). RNA silencing is an antiviral defense mechanism.…”

Section: Discussionmentioning

confidence: 99%

“…Both of these alphasatellites were ϳ1.3 kb long and shared 77% identity with one another. In a similarity search, one alphasatellite showed an identity of 99% with Cotton leaf curl Multan alphasatellite (CLCuMA) from okra (46), and the other showed an identity of 93% with Guar leaf curl alphasatellite (GLCuA) from Cyamopsis tetragonoloba (38), thus establishing the presence of CLCuMA and GLCuA in wheat. Both of these alphasatellite molecules possessed three highly conserved features: a loop sequence TAGTATTAC (nonanucleotide sequence), an adenine (A)-rich region, and a gene encoding a Rep (Fig.…”

Section: Detection Of Wdiv and Satellites In Wheatmentioning

confidence: 99%

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Association of Satellites with a Mastrevirus in Natural Infection: Complexity of Wheat Dwarf India Virus Disease

Kumar

Singh

et al. 2014

J Virol

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In contrast to begomoviruses, mastreviruses have not previously been shown to interact with satellites. This study reports the first identification of the association of satellites with a mastrevirus in field-grown plants. Two alphasatellite species were detected in different field samples of wheat infected with Wheat dwarf India virus (WDIV), a Cotton leaf curl Multan alphasatellite (CLCuMA) and a Guar leaf curl alphasatellite (GLCuA). In addition to the alphasatellites, a betasatellite, Ageratum yellow leaf curl betasatellite (AYLCB), was also identified in the wheat samples. No begomovirus was detected in the wheat samples, thus establishing association of the above-named satellites with WDIV. Agrobacterium-mediated inoculation of WDIV in wheat, in the presence of either of the alphasatellites or the betasatellite, resulted in infections inducing more severe symptoms. WDIV efficiently maintained each of the alphasatellites and the betasatellite in wheat. The satellites enhanced the level of WDIV DNA in wheat. Inoculation of the satellites isolated from wheat with various begomoviruses into Nicotiana tabacum demonstrated that these remain capable of interacting with the viruses with which they were first identified. Virus-specific small RNAs accumulated in wheat upon infection with WDIV but were lower in abundance in plants coinfected with the satellites, suggesting that both the alphasatellites and the betasatellite suppress RNA silencing. These results suggest that the selective advantage for the maintenance of the alphasatellites and the betasatellite by WDIV in the field is in overcoming RNA silencing-mediated host defense. IMPORTANCE Wheat is the most widely cultivated cereal crop in the world. A number of viruses are important pathogens of wheat, including the viruses of the genus

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Section: Discussionmentioning

confidence: 99%

Section: Detection Of Wdiv and Satellites In Wheatmentioning

confidence: 99%

Association of Satellites with a Mastrevirus in Natural Infection: Complexity of Wheat Dwarf India Virus Disease

Kumar

Singh

et al. 2014

J Virol

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“…Moreover, typical OLCD symptom was observed only when a betasatellite was co-inoculated with the infectious clones of OYCrV or CLCuGV. Using RCA, Kumar et al ( 2012 ) have constructed the infectious clones and presence of identical viral DNAs were confirmed by sequencing. Further, infectivity testing of tobacco and okra plants using a mixture of begomovirus and alpha and betasatellite infectious clones resulted in typical viral symptoms.…”

Section: Rolling Circle Amplification (Rca) and Infectious Clones As mentioning

confidence: 99%

Biotechnological Advancements and Begomovirus Management in Okra (Abelmoschus esculentus L.): Status and Perspectives

et al. 2017

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Despite the importance of okra, as one of the important vegetable crop, very little attention has been paid to its genetic improvement using advanced biotechnological tools. The exploitation of marker assisted breeding in okra is often limited due to the availability of a few molecular markers, the absence of molecular genetic-map(s), and other molecular tools. Chromosome linkage-groups were not yet constructed for this crop and reports on marker development are very scanty and mostly hovering around cultivar characterization. Besides, very little progress has been observed for transgenic development. However, high throughput biotechnological tools like chromosome engineering, RNA interference (RNAi), marker-assisted recurrent selection (MARS), genome-wide selection (GWS), targeted gene replacement, next generation sequencing (NGS), and nanobiotechnology can provide a rapid way for okra improvement. Further, the etiology of many deadly viral diseases like the yellow vein mosaic virus (YVMV) and okra enation leaf curl virus (OELCV) in okra is broadly indistinct and has been shown to be caused by various begomovirus species. These diseases cause systemic infections and have a very effective mode of transmission; thus, preventing their spread has been very complicated. Biotechnological interventions have the potential to enhance okra production even under different viral-stress conditions. In this background, this review deals with the biotechnological advancements in okra per se along with the begomoviruses infecting okra, and special emphasis has been laid on the exploitation of advanced genomic tools for the development of resistant varieties.

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