First Report of <i>Rhizopus oryzae</i> Causing Postharvest Fruit Rot on Pear in China

Khokhar, Ibatsam; Yang, Jiakuan; Mukhtar, Irum; Wang, J. H.; Ruth, N.; Eltoukhy, Adel; Fan, Shaohui; Li, X. J.; Yan, Yu

doi:10.1094/pdis-11-18-1993-pdn

Cited by 6 publications

(1 citation statement)

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“…Rhizopus is fast-growing and their infection of fruits and other types of food is characterized by white mycelia and black sporangiospores during the decay process; another principal characteristic is the formation of rhizoids [ 39 , 40 ]. Some Rhizopus species, such as R. oryzae and R. stolonifera , are weak parasites of ripening honeybee-pollinated fruit crops, including apple, peach, strawberry, citrus, persimmon, pear, and pumpkin [ 39 , 41 , 42 , 43 , 44 ]. Following the bees’ foraging activities on infected crops, fungal spores gain entry and become established in the beehive by dissemination through direct contact and food contamination [ 45 , 46 ].…”

Section: Introductionmentioning

confidence: 99%

A New Isolated Fungus and Its Pathogenicity for Apis mellifera Brood in China

Aynalem,

Meng,

Getachew

et al. 2024

Microorganisms

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In this article, we report the pathogenicity of a new strain of fungus, Rhizopus oryzae to honeybee larvae, isolated from the chalkbrood-diseased mummies of honeybee larvae and pupae collected from apiaries in China. Based on morphological observation and internal transcribed spacer (ITS) region analyses, the isolated pathogenic fungus was identified as R. oryzae. Koch’s postulates were performed to determine the cause-and-effect pathogenicity of this isolate fungus. The in vitro pathogenicity of this virulent fungus in honeybees was tested by artificially inoculating worker larvae in the lab. The pathogenicity of this new fungus for honeybee larvae was both conidial-concentration and exposure-time dependent; its highly infectious and virulent effect against the larvae was observed at 1 × 105 conidia/larva in vitro after 96 h of challenge. Using probit regression analysis, the LT50 value against the larvae was 26.8 h at a conidial concentration of 1 × 105 conidia/larva, and the LC50 was 6.2 × 103 conidia/larva. These results indicate that the new isolate of R. oryzae has considerable pathogenicity in honeybee larvae. Additionally, this report suggests that pathogenic phytofungi may harm their associated pollinators. We recommend further research to quantify the levels, mechanisms, and pathways of the pathogenicity of this novel isolated pathogen for honeybee larvae at the colony level.

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