First Report of <i>Shallot yellow stripe virus</i> in <i>Lilium lancifolium</i> in China

Yang, Ping; He, Guoren; Cao, Yang; Xu, L. F.; Jun, Ming

doi:10.1094/pdis-11-18-1994-pdn

Cited by 1 publication

(3 citation statements)

References 6 publications

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“…Viral diseases frequently occur in lily plants, and they affect bulb and cut-flower production [4][5][6][7][8][9]. In this study, 92 lily samples in China were tested using the developed multiplex TaqMan real-time PCR assay.…”

Section: Discussionmentioning

confidence: 99%

“…Lily cultivars are usually propagated vegetatively, resulting in virus transmission from generation to generation. Among viruses infecting lilies, Lily symptomless virus (LSV; genus Carlavirus, family Flexiviridae), Cucumber mosaic virus (CMV; genus Cucumovirus, family Bromoviridae), Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae), Shallot yellow stripe virus (SYSV; genus Potyvirus, family Potyviridae), and Plantago asiatica mosaic virus (PlAMV; genus Potexvirus, family Alphaflexiviridae) are five economically important viruses [4][5][6][7][8][9]. LSV-infected plants show mild, pale vein clearing and mottle on leaves, which generally turn yellow fairly quickly [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…CMV-infected lily leaves show chlorotic or yellow spotting, inter-veinal striping or vein-clearing and sometimes malformations [6,7]. Leaves infected with SYSV show yellow stripe symptoms [8]. When infected with PlAMV, leaves of infected plants are often brittle, flower production is declined, and the plants gradually die [9].…”

Section: Introductionmentioning

confidence: 99%

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Application of Multiplex TaqMan Real-Time PCR Assay in Survey of Five Lily Viruses Infecting Lilium spp.

Song

Jun

2021

Agronomy

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Lily symptomless virus (LSV), Lily mottle virus (LMoV), Cucumber mosaic virus (CMV), Shallot yellow stripe virus (SYSV), and Plantago asiatica mosaic virus (PlAMV) are five of the economically important viruses infecting lilies (Lilium spp.) worldwide. In order to prevent the occurrence and spread of these viruses, it is necessary to develop a rapid, effective, and sensitive detection method for the simultaneous detection and specific quantification of these viruses. In this study, specific primers and probes for multiplex TaqMan real-time PCR assays designed from conserved regions of the coat protein sequence of each virus were used for the simultaneous detection of these viruses in lilies (Lilium spp.). The optimal concentration of primers and probes and reaction annealing temperature were 20 µM and 55.9 °C, respectively. The detection limits of the assay were 1.33 × 102, 1.27 × 101, 1.28 × 101, 2.33 × 102, and 2.01 × 102 copies·μL−1 for LSV, LMoV, CMV, SYSV, and PlAMV, respectively. Specificity was determined using seven viral pathogens of lilies. Variability tests of intra- and inter-assays showed high reproducibility with coefficients of variation <2%. The multiplex TaqMan real-time PCR assay was used to detect these viruses from lily samples in China. In brief, our developed assay showed high specificity, sensitivity, and reproducibility for the simultaneous detection and differentiation of five lily-infecting viruses and can be used for certification and quarantine programs.

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