First Report of <i>Trichosporon ovoides</i> Isolated from the Home of a Summer‐Type Hypersensitivity Pneumonitis Patient

Sugita, Takashi; Nishikawa, Akemi; Ikeda, Reiko; Saitô, Takako; Sakashita, Hiroyuki; Sakai, Youko; Yoshizawa, Yasuyuki

doi:10.1111/j.1348-0421.1998.tb02312.x

Cited by 9 publications

(5 citation statements)

References 16 publications

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“…These procedures are capable of characterizing the majority of species of Trichosporon, including those pathogenic for humans (26,28,30). The results obtained with these techniques have led to the reclassification of both clinical and environmental isolates of these species, the design of a nested PCR assay for the detection of DNA in sera for the diagnosis of deep-seated trichosporonosis, description of the first bloodstream infection due T. asteroides, and the association of T. ovoides with hypersensitivity pneumonitis (15,(27)(28)(29). Recently, DNA-based procedures for the identification of Trichosporon have been stepped up by the sequence analysis of the rRNA IGS1, which provides a more powerful and alternative method for distinguishing between phylogenetically closely related species (26).…”

Section: Discussionmentioning

confidence: 99%

Susceptibility Patterns and Molecular Identification of Trichosporon Species

Rodríguez-Tudela

Diaz-Guerra

Mellado

et al. 2005

Antimicrob Agents Chemother

175

129

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The physiological patterns, the sequence polymorphisms of the internal transcriber spacer (ITS), and intergenic spacer regions (IGS) of the rRNA genes and the antifungal susceptibility profile were evaluated for their ability to identify Trichosporon spp. and their specificity for the identification of 49 clinical isolates of Trichosporon spp. Morphological and biochemical methodologies were unable to differentiate among the Trichosporon species. ITS sequencing was also unable to differentiate several species. However, IGS1 sequencing unambiguously identified all Trichosporon isolates. Following the results of DNA-based identification, Trichosporon asahii was the species most frequently isolated from deep sites (15 of 25 strains; 60%). In the main, other Trichosporon species were recovered from cutaneous samples. The majority of T. asahii, T. faecale, and T. coremiiforme clinical isolates exhibited resistance in vitro to amphotericin B, with geometric mean (GM) MICs >4 g/ml. The other species of Trichosporon did not show high MICs of amphotericin B, and GM MICs were <1 g/ml. Azole agents were active in vitro against the majority of clinical strains. The most potent compound in vitro was voriconazole, with a GM MIC <0.14 g/ml. The sequencing of IGS correctly identified Trichosporon isolates; however, this technique is not available in many clinical laboratories, and strains should be dispatched to reference centers where these complex methods are available. Therefore, it seems to be more practical to perform antifungal susceptibility testing of all isolates belonging to Trichosporon spp., since correct identification could take several weeks, delaying the indication of an antifungal agent which exhibits activity against the infectious strain.

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Section: Discussionmentioning

confidence: 99%

Susceptibility Patterns and Molecular Identification of Trichosporon Species

Rodríguez-Tudela

Diaz-Guerra

Mellado

et al. 2005

Antimicrob Agents Chemother

175

129

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“…In 1992, it had been separated from Trichosporon cutaneum and became an independent species 1 . It had been reported in Japan that T. asahii could cause cutaneous infection in leukemia patients, 2,4 lung infection in patients with summer‐type hypersensitivity pneumonitis, 5 and experimental disseminated trichosporonosis in mouse 6 . We treated a patient with disseminated infection caused by T. asahii in 2000.…”

Section: Introductionmentioning

confidence: 99%

Disseminated trichosporonosis in China

Wang

Song

et al. 2003

Mycoses

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A 20-year-old female patient presented with erythematous plaques on the nose which were progressively spreading to the trunk and the extremities, sometimes with erosions and scars. The patient was misdiagnosed as having seborrhoeic dermatitis and subacute cutaneous lupus erythematosus. The histopathological biopsy revealed mycotic infectious granuloma. Samples taken from skin lesions and other locations grew Trichosporon asahii in cultures. The identification was confirmed by molecular biological methods. The patient was treated successfully with liposomal amphotericin B in combination with fluconazole orally.

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“…While T. dermatis (serotype I), T. asahii (serotype II), and T. montevideense (serotype III) were the principal causative antigens identified in our mycological investigation, other species have also been considered to cause SHP. Sugita et al (17) isolated T. ovoides (serotype II) from an SHP patient's house, and the crude antigen extracted from cultures of that isolate provoked a positive reaction in the patient when used in an inhalation challenge (personal communication). Although serotype I-III species are widely distributed in the environment, they were not isolated from the houses of patients in the present study.…”

Section: Ovoides)mentioning

confidence: 99%

Analysis of Trichosporon Isolates Obtained from the Houses of Patients with Summer-Type Hypersensitivity Pneumonitis

Sugita¹,

Ikeda²,

Nishikawa

2004

J Clin Microbiol

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Summer-type hypersensitivity pneumonitis (SHP) is type III or IV allergies developed by repeated inhalation of arthroconidia of Trichosporon species. We identified 105 strains obtained from the homes of 36 SHP patients by analysis of the intergenic spacer (IGS) 1 region, which is located between the 26S and 5S rRNA genes; in addition, we analyzed the IGS genotypes of the strains. Serologically, Trichosporon species are classified as serotype I, II, III, or I-III. Of the 105 strains, 43 (41.1%), 53 (50.5%), and 9 (8.6%) strains were isolated as serotypes I, II, and III, respectively. Serotype I, II, and III strains were recovered from 19 (52.8%), 29 (80.6%), and 4 (11.1%) of the 36 houses of SHP patients, respectively. No serotype I-III strains were isolated from the houses. Of 43 serotype I strains, 42 (97.7%) were identified as Trichosporon dermatis, and the remaining one was T. terricola. Of 53 serotype II strains, 37 (69.8%) were identified as T. asahii, and the remaining serotype II isolates were T. aquatile (1.9%), T. coremiiforme (7.5%), T. faecale (1.9%), T. japonicum (15.1%), and T. ovoides (3.8%). There were nine serotype III strains comprised of T. montevideense (77.8%) and T. domesticum (22.2%). Intraspecies diversity was found only in T. asahii. This microorganism also causes opportunistic infections (trichosporonosis); seven genotypes of its IGS 1 region have been identified. While the strains of T. asahii obtained from Japanese patients with trichosporonosis were genotype I, the strains from the houses of SHP patients were genotype III. Based on our analysis, we conclude that the strains that play the most significant roles in the development of SHP are T. dermatis, T. asahii genotype 3, and T. montevideense, representing serotypes I, II, and III, respectively.

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First Report of Trichosporon ovoides Isolated from the Home of a Summer‐Type Hypersensitivity Pneumonitis Patient

Cited by 9 publications

References 16 publications

Susceptibility Patterns and Molecular Identification of Trichosporon Species

Susceptibility Patterns and Molecular Identification of Trichosporon Species

Disseminated trichosporonosis in China

Analysis of Trichosporon Isolates Obtained from the Houses of Patients with Summer-Type Hypersensitivity Pneumonitis

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