First Report of Isolation of Gallibacterium anatis from Layer Chickens in Morocco with Decrease in Laying Performance

Nassik, Saadia; Tallouzt, Siham; Karbach, Nsrine; Touzani, Charifa Drissi; Bidoudan, Yassmina; Aamarine, Nadim; Heß, Claudia

doi:10.1637/aviandiseases-d-19-00119

Cited by 12 publications

(12 citation statements)

References 23 publications

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“…Our results are nearly agreed with those reported by Krishnegowda, 2 Sorour, 37 and Nassik. 38 The rtx -like toxin ( gtx A) is one of the main virulence determinants of G. anatis encoded by the gtx A gene. 27 The gtx A toxin was demonstrated for the first time in a hemolytic G. anatis strain isolated from diseased chicken in 2010 in Denmark.…”

Section: Discussionmentioning

confidence: 99%

Sequence Analysis, Antibiogram Profile, Virulence and Antibiotic Resistance Genes of XDR and MDR Gallibacterium anatis Isolated from Layer Chickens in Egypt

Algammal¹,

Hashem²,

Alfifi³

et al. 2022

IDR

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Background Gallibacterium anatis is incriminated frequently in severe economic losses and mortalities in the poultry industry. This study aimed to detect the prevalence of G. anatis in layer chickens, sequence analysis, the antibiogram profiles, and PCR screening of virulence determinants and antibiotic resistance genes. Methods Accordingly, 300 samples (tracheal swabs, ovary and oviduct, and lung) were randomly collected from 100 diseased layer chickens from private commercial layer farms at Elsharkia Governorate, Egypt. The bacteriological examination was carried out. The retrieved isolates were tested for 16S rRNA-23S rRNA gene sequencing, antibiogram profiling, PCR screening of virulence ( gtx A, fif A, and gyr B), and antibiotic resistance genes ( bla ROB , aph A1, tet B, and tet H). Results The prevalence of G. anatis was 25% in the examined diseased layer chickens. The sequence analyses emphasized that the tested strains derived from a common ancestor and exhibited a notable genetic similarity with other G. anatis strains from USA, China, and Denmark. The isolated G. anatis strains were highly resistant to sulfamethoxazole-trimethoprim, oxytetracycline, penicillin, ampicillin, kanamycin, neomycin, and erythromycin. The PCR revealed that the retrieved G. anatis strains carried gtx A, gyr B, and fif A virulence genes with a prevalence of 100%, 100%, and 38.3%, respectively. Approximately 30.1% of the retrieved G. anatis isolates were XDR to six antimicrobial classes and harbored bla ROB , aph A1, and tet B resistance genes. Moreover, 20.5% of the isolated G. anatis strains were MDR to three different classes and carried bla ROB and tet H resistance genes. Conclusion Briefly, this study emphasized the existence of XDR and MDR G. anatis strains in poultry. Florfenicol and norfloxacin displayed a promising antimicrobial effect against the emerging XDR and MDR G. anatis in poultry. The emergence of XDR and MDR G. anatis is considered a public health alarm.

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Section: Discussionmentioning

confidence: 99%

Sequence Analysis, Antibiogram Profile, Virulence and Antibiotic Resistance Genes of XDR and MDR Gallibacterium anatis Isolated from Layer Chickens in Egypt

Algammal¹,

Hashem²,

Alfifi³

et al. 2022

IDR

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“…Gallibacterium have been reported across the world, such as European countries including Switzerland, Denmark, Germany, Norway, England, Sweden, Czech Republic and Austria (Mr az et al 1976;Bisgaard 1977;Mirle et al 1991;Neubauer et al 2009;Jones et al 2013); African countries like Nigeria, Egypt, Morocco (Addo & Mohan 1985;Elbestawy et al 2018;Nassik et al 2019); Asian countries including China, Taiwan, Iran, Syria, India and Japan (Suzuki et al 1996;Guo 2011;Huangfu et al 2012;Ataei et al 2017;Zhang et al 2017;Singh et al 2018); American countries such as the USA, Canada, Colombia, Peru and Mexico (Hacking & Pettit 1974;Shaw et al 1990;Bojesen et al 2008;Mendoza et al 2014;Paudel, Liebhart, Aurich, et al 2014;Ch avez et al 2017); and Australia (Jordan et al 2005). G. anatis, particularly biovar hemolytica, has recently gained importance due to its ability to cause both respiratory and reproductive infections, and its occurrence in countries on various continents including Europe, Asia, America, and Africa (Neubauer et al 2009).…”

Section: Epidemiology Prevalence and Host Rangementioning

confidence: 99%

Etiology, epidemiology, pathology, and advances in diagnosis, vaccine development, and treatment ofGallibacterium anatisinfection in poultry: a review

Krishnegowda

Dhama

Kumar

et al. 2020

Veterinary Quarterly

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Gallibacterium anatis is a Gram-negative bacterium of the Pasteurellaceae family that resides normally in the respiratory and reproductive tracts in poultry. It is a major cause of oophoritis, salpingitis, and peritonitis, decreases egg production and mortality in hens thereby severely affecting animal welfare and overall productivity by poultry industries across Europe, Asia, America, and Africa. In addition, it has the ability to infect wider host range including domesticated and free-ranging avian hosts as well as mammalian hosts such as cattle, pigs and human. Evaluating the common virulence factors including outer membrane vesicles, fimbriae, capsule, metalloproteases, biofilm formation, hemagglutinin, and determining novel factors such as the RTX-like toxin GtxA, elongation factor-Tu, and clustered regularly interspaced short palindromic repeats (CRISPR) has pathobiological, diagnostic, prophylactic, and therapeutic significance. Treating this bacterial pathogen with traditional antimicrobial drugs is discouraged owing to the emergence of widespread multidrug resistance, whereas the efficacy of preventing this disease by classical vaccines is limited due to its antigenic diversity. It will be necessary to acquire in-depth knowledge on important virulence factors, pathogenesis and, concerns of rising antibiotic resistance, improvised treatment regimes, and novel vaccine candidates to effectively tackle this pathogen. This review substantially describes the etio-epidemiological aspects of G. anatis infection in poultry, and updates the recent development in understanding the pathogenesis, organism evolution and therapeutic and prophylactic approaches to counter G. anatis infection for safeguarding the welfare and health of poultry.

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“…Susceptibilidad a antibióticos en bacterias Gram positivas. ), lo cual coincide con la resistencia reportada en distintos tipos de muestras (cornetes, tráquea, pulmones, hendidura palatina, hígado, bazo, riñones, folículos, peritoneo, ovario, proventrículo, laringe, corazón, saco aéreo, cerebro, ojo y oviducto) de gallinas ponedoras, pollos de engorda y reproductoras; además, presentó sensibilidad a la familia de los fenicoles y gentamicina (Osuna et al, 2017;El-Adawy et al, 2018;Elbestawy et al, 2018;Nassik et al, 2019).…”

Section: Bacteriasunclassified

“…Respecto a Corynebacterium spp se describió resistencia a macrólidos, clindamicina, trimetoprim/sulfametoxazol, quinolonas y/o rifampicina (Yang et al, 2018). Por otra parte, se reportó resistencia a los betalactámicos (penicilina y ampicilina), tetraciclina, tilosina, novobiocina, sulfonamida, lincomicina, enrofloxacina, florfenicol, cefotaxima, clindamicina, sulfatiazol, penicilina, norfloxacina y cefalotina en Gallibacterium anatis (antes Mannheimia haemolytica) (Osuna et al, 2017;El-Adawy et al, 2018;Elbestawy et al, 2018;Nassik et al, 2019;Krishnegowda et al, 2020). El objetivo del presente estudio fue identificar las bacterias aisladas de tráquea de gallinas ponedoras y determinar su perfil de resistencia a antibióticos.…”

Section: Introductionunclassified

Identificación y resistencia antimicrobiana de bacterias de tráquea de gallinas ponedoras

2022

Abanico Vet

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En la producción avícola los problemas respiratorios bacterianos son causa de pérdidas económicas por disminución de la producción, aumento del costo por tratamiento con antibióticos y primera causa de muerte en gallinas ponedoras. La presencia de patógenos implica una distribución en las unidades de producción y su identificación por pruebas bioquímicas permite la caracterización a nivel especie y su tratamiento adecuado. El objetivo del presente estudio fue identificar las bacterias aisladas de tráquea de gallinas ponedoras y determinar su perfil de resistencia a antibióticos. En una granja de producción intensiva, se tomaron muestras de tráquea de gallinas; la detección bacteriana se realizó con el aislamiento, identificación colonial, microscópica y pruebas bioquímicas, además se determinó la prueba de susceptibilidad antimicrobiana. Se obtuvieron 32 aislados correspondientes a cinco tipos de colonias bacterianas con morfología de cocos y bacilos Gram positivos (Staphylococcus aureus, Staphylococcus epidermidis y Corynebacterium spp) y cocobacilos Gram negativos (Pasteurella multocida y Gallibacterium anatis). S. aureus, S. epidermidis y Corynebacterium spp, mostraron 100% de resistencia a glucopéptidos, además P. multocida y G. anatis, a ampicilina (betalactámicos) y quinolonas (100%). Las bacterias aisladas mostraron resistencia y multirresistencia a antibióticos, con implicaciones para la avicultura y la salud pública.

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First Report of Isolation of Gallibacterium anatis from Layer Chickens in Morocco with Decrease in Laying Performance

Cited by 12 publications

References 23 publications

Sequence Analysis, Antibiogram Profile, Virulence and Antibiotic Resistance Genes of XDR and MDR Gallibacterium anatis Isolated from Layer Chickens in Egypt

Sequence Analysis, Antibiogram Profile, Virulence and Antibiotic Resistance Genes of XDR and MDR Gallibacterium anatis Isolated from Layer Chickens in Egypt

Etiology, epidemiology, pathology, and advances in diagnosis, vaccine development, and treatment ofGallibacterium anatisinfection in poultry: a review

Identificación y resistencia antimicrobiana de bacterias de tráquea de gallinas ponedoras

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