First Report of Klebsiella oxytoca Strain Coproducing KPC-2 and IMP-8 Carbapenemases

Li, Bin; Sun, Jingyong; Han, Lizhong; Huang, Xing; Ni, Yizhao

doi:10.1128/aac.01670-10

Cited by 33 publications

(21 citation statements)

References 28 publications

(34 reference statements)

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“…Transfer of carbapenem resistance determinants was carried out using a broth culture mating method (Li et al 2011). Transferred plasmids were assigned to the incompatibility groups by a PCR-based replicon typing as described (Carattoli et al 2005).…”

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High prevalence of metallo-β-lactamase among carbapenem-resistant Klebsiella pneumoniae in a teaching hospital in China

Zhao

et al. 2014

Can. J. Microbiol.

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The aim of this study was to characterize the carbapenemases in carbapenem-resistant Klebsiella pneumoniae (CR-KP) from a Chinese teaching hospital. A total of 40 CR-KPs were screened for the presence of carbapenemases. Minimum inhibitory concentrations were determined by agar dilution. The modified Hodge test was used for the detection of carbapenemase production. Carbapenemase, extended-spectrum β-lactamase, and AmpC genes were detected using polymerase chain reaction (PCR) and sequencing. A conjugation test was performed using a broth culture mating method, transferred plasmids were typed by PCR-based replicon typing, and clonal relatedness was investigated by enterobacterial repetitive intergenic consensus sequences PCR (ERIC-PCR) and multilocus sequence typing (MLST). The results revealed that modified Hodge test was positive for 28 CR-KPs, and CR-KPs exhibited high resistance rates against various antibiotics, except colistin (5.0%) and tigecycline (22.5%). ERIC and MLST profiles showed no clonal outbreak. PCR demonstrated a high prevalence rate (55.0%, 22/40) of metallo-β-lactamases (MBLs) in CR-KPs. IMP-4, IMP-8, NDM-1, and KPC-2 were identified in 14 (35.0%), 7 (17.5%), 2 (5.0%), and 7 (17.5%) isolates, respectively. Notably, 2 CR-KPs coproduced 2 carbapenemases simultaneously (IMP-8/NDM-1 and IMP-4/KPC-2). In vitro transfer of carbapenem resistance was successful for 11 MBL-producing CR-KPs. The extended spectrum β-lactamase genes were detected in 30 (75.0%) of these CR-KPs. To the best of our knowledge, this is the first report focusing on carbapenem resistance in K. pneumoniae due to metalloenzymes in China. Screening and surveillance of MBLs in Enterobacteriaceae is urgently needed in this region to control and prevent the spread of these resistance determinants.

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High prevalence of metallo-β-lactamase among carbapenem-resistant Klebsiella pneumoniae in a teaching hospital in China

Zhao

et al. 2014

Can. J. Microbiol.

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“…Most IMP, VIM, and GIM carbapenemase genes are associated with sul1-type class I integrons, but recently, carbapenemases have been found to occur on Tn402-type class 1 integrons with an intact tniRQBA module. Examples include VIM-1 in P. aeruginosa (13), VIM-2 in P. aeruginosa (14,15) and in Pseudomonas putida (16), and IMP-8 in Klebsiella oxytoca (17), among others.…”

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Complete Sequence of pOZ176, a 500-Kilobase IncP-2 Plasmid Encoding IMP-9-Mediated Carbapenem Resistance, from Outbreak Isolate Pseudomonas aeruginosa 96

Xiong

Alexander

Jennifer

et al. 2013

Antimicrob Agents Chemother

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Pseudomonas aeruginosa 96 (PA96) was isolated during a multicenter surveillance study in Guangzhou, China, in 2000. Wholegenome sequencing of this outbreak strain facilitated analysis of its IncP-2 carbapenem-resistant plasmid, pOZ176. The plasmid had a length of 500,839 bp and an average percent G؉C content of 57%. Of the 618 predicted open reading frames, 65% encode hypothetical proteins. The pOZ176 backbone is not closely related to any plasmids thus far sequenced, but some similarity to pQBR103 of Pseudomonas fluorescens SBW25 was observed. Two multiresistant class 1 integrons and several insertion sequences were identified. The bla IMP-9 -carrying integron contained aacA4¡bla IMP-9 ¡aacA4, flanked upstream by Tn21 tnpMRA and downstream by a complete tni operon of Tn402 and a mer module, named Tn6016. The second integron carried aacA4¡catB8a¡bla OXA-10 and was flanked by Tn1403-like tnpRA and a sul1-type 3= conserved sequence (3=-CS), named Tn6217. Other features include three resistance genes similar to those of Tn5, a tellurite resistance operon, and two pil operons. The replication and maintenance systems exhibit similarity to a genomic island of Ralstonia solanacearum GM1000. Codon usage analysis suggests the recent acquisition of bla IMP-9 . The origins of the integrons on pOZ176 indicated separate horizontal gene transfer events driven by antibiotic selection. The novel mosaic structure of pOZ176 suggests that it is derived from environmental bacteria.

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“…The emergence of KPC-producing K. pneumoniae, often associated with smaller outbreaks, has recently been reported also from several European countries, including Germany, Italy, Poland, Switzerland, and France (9,10,13,17,23). In contrast, reports of KPC-producing Klebsiella oxytoca are rare (5,11,19,25). Most of these reports cover one or two isolates and, to the best of our knowledge, no outbreak caused by KPC-producing K. oxytoca has been described yet.…”

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confidence: 78%

Nosocomial Outbreak of Klebsiella pneumoniae Carbapenemase-Producing Klebsiella oxytoca in Austria

Hoenigl

Valentin

Zarfel

et al. 2012

Antimicrob Agents Chemother

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To date, no outbreak of carbapenemase-producing bacteria has been reported for Austria. While outbreaks ofKlebsiella pneumoniaecarbapenemase (KPC)-producingK. pneumoniaehave been increasingly reported, no outbreak caused by KPC-producingKlebsiella oxytocahas been described yet, to the best of our knowledge. We report an outbreak of KPC-producingK. oxytoca. In 5 months, 31 KPC-producingKlebsiella oxytocastrains were isolated from five patients. All patients were admitted to the same medical intensive care unit in Austria.

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First Report of Klebsiella oxytoca Strain Coproducing KPC-2 and IMP-8 Carbapenemases

Cited by 33 publications

References 28 publications

High prevalence of metallo-β-lactamase among carbapenem-resistant Klebsiella pneumoniae in a teaching hospital in China

High prevalence of metallo-β-lactamase among carbapenem-resistant Klebsiella pneumoniae in a teaching hospital in China

Complete Sequence of pOZ176, a 500-Kilobase IncP-2 Plasmid Encoding IMP-9-Mediated Carbapenem Resistance, from Outbreak Isolate Pseudomonas aeruginosa 96

Nosocomial Outbreak of Klebsiella pneumoniae Carbapenemase-Producing Klebsiella oxytoca in Austria

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