First report of L1014F kdr mutation in <i>Culex quinquefasciatus</i> in Mexico

Ponce, Gustavo; Sánchez, Iram Pablo Rodríguez; García, Selene M.; Torrado, José Manuel; Lozano-Fuentes, Saúl; López-Monroy, Beatriz; Flores, Adriana E.

doi:10.1111/1744-7917.12218

Cited by 12 publications

(11 citation statements)

References 36 publications

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“…A modest frequency of kdr mutations was observed in the four resistant populations compared with susceptible populations. This modest kdr mutation frequency may be related to intensive pyrethroid usage in the past two decades in an area where major campaigns were carried out to eradicate mosquitoes, in addition to the wide adoption of pyrethroids for spraying economic crops [3, 16, 39–41]. Pyrethroids have been the major insecticide employed for aerial spraying for adult mosquito control and agriculture pest control.…”

Section: Discussionmentioning

confidence: 99%

Trends in insecticide resistance in Culex pipiens pallens over 20 years in Shandong, China

et al. 2019

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Background Culex pipiens pallens is the most abundant Culex mosquito species in northern China and is an important vector of bancroftian filariasis and, potentially, West Nile virus. Insecticides, particularly pyrethroids, are widely used for adult mosquito control. Insecticide resistance has become common in several mosquito species, and vector control is the main method currently available to prevent disease transmission. The voltage-gated sodium channel ( Vgsc ) gene is the target site of pyrethroids, and mutations in this gene cause knockdown resistance ( kdr ). Methods Culex pipiens pallens larvae were collected from May to November over two decades, from 1992 to 2018, in four cities in Shandong Province, China. The World Health Organization (WHO) standard resistance bioassay was applied to test the resistance levels of Cx. p. pallens larvae to five different insecticides and to test deltamethrin resistance in adults, using the F1 generation. Mutations at Vgsc codon 1014 were also screened in 471 adult samples collected in 2014 to determine the association between kdr mutations and phenotypic resistance. Results Larval resistance against deltamethrin showed an increasing trend from the 1990s until 2018, which was statistically significant in all populations; resistance to cypermethrin increased significantly in mosquitoes from the Zaozhuang population. However, larval resistance to other insecticides remained relatively stable. Larval resistance against deltamethrin was consistent with adult bioassays in 2014, in which all tested populations were highly resistant, with mortality rates ranging from 39.4 to 55.23%. The L1014S and L1014F mutations were both observed in five Cx. p. pallens populations, with L1014F significantly associated with deltamethrin resistance. Conclusions The long-term dataset from Shandong demonstrates major increases in pyrethroid resistance over a 20-year period. The L1014F kdr mutation may be considered a viable molecular marker for monitoring pyrethroid resistance in Cx. p. pallens. Electronic supplementary material The online version of this article (10.1186/s13071-019-3416-9) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Trends in insecticide resistance in Culex pipiens pallens over 20 years in Shandong, China

et al. 2019

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“…The L1014F kdr mutation has been shown to confer resistance to pyrethroids in Cx . quinquefasciatus [ 33 , 35 ], which can explain the high resistance values obtained for the adulticide deltamethrin (still used in Guadeloupe) for all the studied populations (RR 50 ≥20). This statement is also supported by the PCA analysis that revealed strong positive correlations between deltamethrin resistance and the frequency of the resistant kdr allele [F] (Correlation: r(132) = 0.97, p = 0.031).…”

Section: Discussionmentioning

confidence: 99%

“…Among them, the frequency of most commons target-site modifications described in Cx . quinquefasciatus : (i) the L1014F mutation in the VGSC gene, conferring resistance to pyrethroids and DDT (Dichlorodiphenyltrichloroethane insecticide) [ 33 , 35 , 36 ], and (ii) the G119S mutation in the a ce-1 gene, conferring resistance to temephos and malathion [ 32 ]. In addition, we have determined the constitutive expression levels of five cytochrome P450s that have been shown to be involved in insecticide detoxification in Cx .…”

Section: Introductionmentioning

confidence: 99%

Multiple insecticide resistance in Culex quinquefasciatus populations from Guadeloupe (French West Indies) and associated mechanisms

et al. 2018

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West Nile (WN) virus has been detected in Guadeloupe since 2002. Even if no WN human cases have been detected so far, mosquitoes from Culex genus especially Culex quinquefasciatus are recognized as potential WN vectors in Guadeloupe. To evaluate the impact of local vector control activities on this mosquito species we assessed the resistance levels of Cx. quinquefasciatus populations from three different sites from Guadeloupe (Abymes, Saint François and Gourbeyre) to malathion, temephos and deltamethrin. In addition, the frequencies of the L1014F kdr and the G119S ace-1 mutations were established in Cx. quinquefasciatus populations, as well as the constitutive expressions of five cytochrome P450 genes. Mosquito populations tested displayed high resistance to deltamethrin, moderate resistance to malathion (Abymes, Gourbeyre) and low resistance to temephos (Abymes et Gourbeyre). Molecular analyses revealed high frequencies of both L1014F kdr and G119S ace-1 mutations in Cx. quinquefasciatus populations, as well as overexpression of cytochrome P450 genes CYP9J45, CYP9J40 and CYP6AA7. Finally, deltamethrin resistance and knock-down rates were strongly correlated with the frequency of the resistant kdr and ace-1 alleles, as well as with CYP9J40 overexpression. These results should be taken into account to refine the current vector control strategies to prevent the appearance of Cx. quinquefasciatus-borne diseases in Guadeloupe.

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“…quinquefasciatus , and Ae . aegypti [ 10 , 14 , 15 , 17 , 21 – 24 ]. Other resistance mechanisms include mosquito physiological changes, such as cuticle thickening and digestive tract modification, which may lead to changes in insecticide absorption and penetration, and in behavioral avoidance [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis and RNA interference reveal CYP6A8 and SNPs related to pyrethroid resistance in Aedes albopictus

Bonizzoni

et al. 2018

PLoS Negl Trop Dis

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Wide and improper application of pyrethroid insecticides for mosquito control has resulted in widespread resistance in Aedes albopictus mosquitoes, an important dengue vector. Therefore, understanding the molecular regulation of insecticide resistance is urgently needed to provide a basis for developing novel resistance diagnostic methods and vector control approaches. We investigated the transcriptional profiles of deltamethrin-resistant and -susceptible Ae. albopictus by performing paired-end sequencing for RNA expression analysis. The analysis used 24 independent libraries constructed from 12 wild-caught resistant and 12 susceptible Ae. albopictus female adults. A total of 674,503,592 and 612,512,034 reads were obtained, mapped to the Ae. albopictus genome and assembled into 20,091 Ae. albopictus transcripts. A total of 1,130 significantly differentially expressed genes included 874 up-regulated genes and 256 down-regulated genes in the deltamethrin-resistant individuals. These differentially expressed genes code for cytochrome P450s, cuticle proteins, glutathione S-transferase, serine proteases, heat shock proteins, esterase, and others. We selected three highly differentially expressed candidate genes, CYP6A8 and two genes of unknown function (CCG013931 and CCG000656), to test the association between these 3 genes and deltamethrin resistance using RNAi through microinjection in adult mosquitoes and oral feeding in larval mosquitoes. We found that expression knockdown of these three genes caused significant changes in resistance. Further, we detected 1,162 single nucleotide polymorphisms (SNPs) with a frequency difference of more than 50%. Among them, 5 SNPs in 4 cytochrome P450 gene families were found to be significantly associated with resistance in a genotype-phenotype association study using independent field-collected mosquitoes of known resistance phenotypes. Altogether, a combination of novel individually based transcriptome profiling, RNAi, and genetic association study identified both differentially expressed genes and SNPs associated with pyrethroid resistance in Ae. albopictus mosquitoes, and laid a useful foundation for further studies on insecticide resistance mechanisms.

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First report of L1014F kdr mutation in Culex quinquefasciatus in Mexico

Cited by 12 publications

References 36 publications

Trends in insecticide resistance in Culex pipiens pallens over 20 years in Shandong, China

Trends in insecticide resistance in Culex pipiens pallens over 20 years in Shandong, China

Multiple insecticide resistance in Culex quinquefasciatus populations from Guadeloupe (French West Indies) and associated mechanisms

Comparative transcriptome analysis and RNA interference reveal CYP6A8 and SNPs related to pyrethroid resistance in Aedes albopictus

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