First Report of Leaf Blight of Rice Caused by <i>Pantoea ananatis</i> and <i>Pantoea dispersa</i> in Malaysia

Toh, Wai Keat; Loh, Pek Chin; Wong, Hann Ling

doi:10.1094/pdis-12-18-2299-pdn

Cited by 28 publications

(15 citation statements)

References 4 publications

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“…Similar symptoms had also been detected in another local case at Selangor, Malaysia in 2016. The rice plants showed brownish lines along the leaf margins and eventually the entire leaf became dry [61]. Due to the reduction of the leaf area, the photosynthesis rate is affected, and this inadvertently led to reduced yield and quality of the rice grains.…”

Section: Detrimental Impacts Of Pantoea-rice Plant Interactions Leading To Rice Diseasesmentioning

confidence: 99%

Associations of Pantoea with Rice Plants: As Friends or Foes?

et al. 2021

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Pantoea species are gram-negative bacteria from the Enterobacteriaceae family, generally associated with plants, either as epiphytes or as pathogens. In the last decade, Pantoea species are being regarded as re-emerging pathogens that are the causal agents of various diseases in rice plants. Inherently, they are also known to be opportunistic plant symbionts having the capacity to enhance systemic resistance and increase the yield of rice plants. It is unclear how they can express both beneficial and pathogenic traits, and what factors influence and determine the outcome of a particular Pantoea–rice plant interaction. This review aims to compare the characteristics of rice plant-beneficial and pathogenic strains belonging to the Pantoea species and gain new insights, enabling distinction among the two types of plant–microbe interactions.

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Section: Detrimental Impacts Of Pantoea-rice Plant Interactions Leading To Rice Diseasesmentioning

confidence: 99%

Associations of Pantoea with Rice Plants: As Friends or Foes?

et al. 2021

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“…which play pivotal role in plant genetic engineering. The pBBR1 ori in pYL101C Toh et al (2019) was derived from pBHR1, a derivative of BHR plasmid pBBR122 (MoBiTec, 2017). Plasmid pBHR1 harbours the BHR oriV, enabling it to be maintained at medium high copy number in at least 28 Gram-negative microorganisms.…”

Section: Resultsmentioning

confidence: 99%

“…Here, we validated the host range of pYL101C in E. coli and A. tumefaciens and further expand the list of bacterial hosts by including K. pneumonia and Pantoea spp. (Figure 2), which have identified as rice pathogens (Toh et al, 2019). In addition, two A. tumefaciens strains, C58 and LBA4404, which have different chromosomal background, were successfully tested (Deeba et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Construction of a broad host range expression plasmid vector by Golden Gate cloning

Teo¹,

Toh²,

Tor³

et al. 2021

MJM

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Aims: Expression of recombinant proteins across a range of different host organisms has profound contribution to the advancement in biotechnology. In this study, we aimed to construct a highly versatile broad host range (BHR) expression vector, designated as pYL101C. Methodology and results: The Golden Gate cloning approach was used to construct pYL101C. Key features of pYL101C include a strong integron promoter (PINTc), a BHR pBBR1 origin of replication (ori), gentamycin resistance gene (Gm R ) as a selectable marker and a multiple cloning site (MCS) downstream of the promoter for easy-cloning purpose. To verify the functionality of pYL101C, we cloned the superfolder green fluorescent protein (sfGFP) reporter gene into pYL101C and transferred the resultant recombinant plasmid pYL101C::sfGFP into various Gram-negative bacteria. Transformants obtained stably expressed strong green fluorescence under blue light excitation even without selection after four passages. Conclusion, significance and impact of study: The constructed BHR expression vector, pYL101C and recombinant pYL101C::sfGFP are stable and can be used to monitor the presence of Gram-negative bacteria, such as endophytes and pathogens in their hosts and environment.

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“…For example, P. ananatis can infect a broad range of monocot and dicot plants, including economically important crops [ 21 ], and it caused severe yield losses in onion production as the causal agent of center rot in South Korea [ 22 ]. P. dispersa is also reported as a pathogen threatening rice and onion production in East Asia [ 23 , 24 ]. However, some Pantoea strains have traits that are beneficial for plants.…”

Section: Discussionmentioning

confidence: 99%

A Seed-Borne Bacterium of Rice, Pantoea dispersa BB1, Protects Rice from the Seedling Rot Caused by the Bacterial Pathogen Burkholderia glumae

Kouzai

Akimoto-Tomiyama

2022

Life

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Seedling rot, caused by the bacterial pathogen Burkholderia glumae, is a major disease of rice. It originates from pathogen-contaminated seeds and is thus mainly controlled by pesticide treatments of seeds. We previously demonstrated that the seed-borne bacteria of rice may be a useful and sustainable alternative to pesticides to manage seedling rot, but they are limited in terms of variety. Here, we report that another seed-borne bacterium, Pantoea dispersa BB1, protects rice from B. glumae. We screened 72 bacterial isolates from rice seeds of three genetically different cultivars inoculated or non-inoculated with B. glumae. 16S rRNA gene sequencing revealed that pathogen inoculation affected the composition of culturable seed-borne bacterial communities and increased the presence of Pantoea and Paenibacillus species. Among three Pantoea and Paenibacillus isolates that exhibit tolerance to toxoflavin, a virulence factor of B. glumae, P. dispersa BB1 significantly mitigated the symptoms of rice seedling rot. The culture filtrate of BB1 inhibited the growth of B. glumae in vitro, suggesting that this isolate secretes antibacterial compounds. Seed treatment with BB1 suppressed pathogen propagation in plants, although seed treatment with the culture filtrate did not. Because BB1 did not show pathogenicity in rice, our findings demonstrate that BB1 is a promising biocontrol agent against seedling rot.

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First Report of Leaf Blight of Rice Caused by Pantoea ananatis and Pantoea dispersa in Malaysia

Cited by 28 publications

References 4 publications

Associations of Pantoea with Rice Plants: As Friends or Foes?

Associations of Pantoea with Rice Plants: As Friends or Foes?

Construction of a broad host range expression plasmid vector by Golden Gate cloning

A Seed-Borne Bacterium of Rice, Pantoea dispersa BB1, Protects Rice from the Seedling Rot Caused by the Bacterial Pathogen Burkholderia glumae

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