First Report of Leaf Spot on <i>Sorghum bicolor</i> Caused by <i>Alternaria tenuissima</i> in China

Wei, Jinpeng; Ma, Wei-Si; Liu, X.-Y.; Xu, Jianchu; Zhang, N.; Shao, Wenyong; Chen, R.-N.; Gao, Yu

doi:10.1094/pdis-03-20-0473-pdn

Cited by 2 publications

(1 citation statement)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bitter gourd tissue was cut into small pieces (0.5 cm) and surface sterilized with sodium hypochlorite (NaOCl; 1%) for 1 min after lesion excision, followed by dipping in 70% ethanol for 30 seconds (Wei et al 2020). The pieces were washed with autoclaved dH2O and embedded onto PDA media (Difco) aseptically.…”

Section: Isolation and Maintenance Of Fungimentioning

confidence: 99%

Genetic Analysis and Pathogenic Characterization of Alternaria tenuissima Induced Fruit Rot of Bitter Gourd

et al. 2021

View full text Add to dashboard Cite

Abstract. Iftikhar S, Anwar W, Akhter A, Ali S, Khan HAA, Khurshid M, Haider MS. 2021. Genetic analysis and pathogenic characterization of Alternaria tenuissima induced fruit rot of bitter gourd. Biodiversitas 22: 617-625. Bitter gourd (Momordica charantia Linn.), belongs to Cucurbitaceae family, is widely cultivated in areas with warm climate. In 2017, fruits of bitter gourd-bearing rot symptoms were observed in the Punjab province of Pakistan. The disease-causing fungal isolate was collected from the diseased fruits on potato dextrose agar (PDA). Microscopic examination revealed short conidiophores arose singly, measuring 79.8- 158.5 μm long and 3.94-7.89 μm thick. The size of conidia varied from 25.7 to 46.45 μm and 8.55-14.39 μm in length and width respectively, which were characteristics of Alternaria spp. To confirm the identity and molecular characterization of the isolate, the internal transcribed spacer (ITS) region, translation elongation factor 1 alpha (TEF1-α), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and RNA polymerase II large subunit 2 (RPB2) genes were amplified. The sequence analysis of amplicons and phylogenetic studies specified the homology of isolated Alternaria spp. with the previously reported A. tenuissima in GeneBank. The pathogenicity tests conducted on the fruits of bitter gourd confirmed the disease development with typical Alternaria induced rot symptoms, thus satisfied Koch's postulate. To our knowledge, this is the first record of A. tenuissima causing fruit rot of bitter gourd in Pakistan.

show abstract

Section: Isolation and Maintenance Of Fungimentioning

confidence: 99%

Genetic Analysis and Pathogenic Characterization of Alternaria tenuissima Induced Fruit Rot of Bitter Gourd

et al. 2021

View full text Add to dashboard Cite

show abstract

Pathogen infection influences a distinct microbial community composition in sorghum RILs

et al. 2021

View full text Add to dashboard Cite

Aims The rhizosphere microbiome substantially affects plant health, yet comparatively little is known regarding the foliar community dynamics. Here, we examine the relationship between the microbiota and their response to natural infection by pathogens. Methods We established an experimental system using a set of sorghum recombinant inbred lines (RILS). These RILS included four models denoted as resistant, moderately resistant, susceptible and highly susceptible. A combination of 16S rRNA and ITS gene amplicon approaches was used to assess bacteria and fungi, respectively, in foliar samples. Results We show that the foliar microbiome differs substantially in asymptomatic and symptomatic RILs subsequent to natural infection by pathogens. A significant association was found between plant health and microbial community structure. Our analyses revealed several distinct fungal and bacterial pathogens. These pathogens included Gibberella and Pantoea genera, which were associated with the highly susceptible group. In addition to these pathogens, we also found signatures for Ascochyta, a known plant pathogenic genus. Members of the bacterial genus Methylorubrum and the fungal genus Hannaella, both known to exhibit plant growth-promoting (PGP) traits, were associated with the resistant and moderately resistant groups. These data also reveal numerous highly diverse fungal and bacterial taxa in RILs that did not show symptoms. We also found taxonomic differences between the microbiota hosted by the symptomatic and asymptomatic RILs. Conclusions Together, these data suggest that pathogen infection may result in distinct microbiota. These results suggest that highly diverse microbiome may promote the plants ability to resist the effects of pathogens potentially contributing to plant health.

show abstract

First Report of Leaf Spot on Sorghum bicolor Caused by Alternaria tenuissima in China

Cited by 2 publications

References 4 publications

Genetic Analysis and Pathogenic Characterization of Alternaria tenuissima Induced Fruit Rot of Bitter Gourd

Genetic Analysis and Pathogenic Characterization of Alternaria tenuissima Induced Fruit Rot of Bitter Gourd

Pathogen infection influences a distinct microbial community composition in sorghum RILs

Contact Info

Product

Resources

About