First report of Leishmania (Mundinia) martiniquensis in South American territory and confirmation of Leishbunyavirus infecting this parasite in a mare

Abstract

BACKGROUND Epidemiological data related to leishmaniases or Leishmania infection in horses are scarce. However, studies carried out in different regions in the world showed equids parasitised by Leishmania braziliensis , L. infantum and L. martiniquensis . OBJECTIVES Identify the Leishmania species causing cutaneous leishmaniasis in a mare, living in Rio de Janeiro State (Brazil), and search the presence of Leishmania viruses in the isolated parasite. METHODS Isoenzymes and polymerase chain reaction (PCR) targeting ITSrDNA region followed by sequencing were conducted for typing the isolated parasite. A search for Leishmania virus infection was also performed. FINDINGS The mare presented skin nodules and ulcers in the left pinna caused by Leishmania spp. that was detected by culture and PCR. The parasite was identified as Leishmania ( Mundinia ) martiniquensis , infected by Leishbunyavirus (LBV), representing the first description of this species in South America. The animal travelled to different Brazilian regions, but not to outside the country. MAIN CONCLUSIONS The worldwide distribution of L . martiniquensis and its infection by LBV were confirmed in this study, indicating the autochthonous transmission cycle in Brazil. The clinical profile of the disease in the mare, showing fast spontaneous healing of cutaneous lesions, may indicate that skin lesions related to L. martiniquensis infection in horses might be underdiagnosed.

First report of putative Leishmania RNA virus 2 (LRV2) in Leishmania infantum strains from canine and human visceral leishmaniasis cases in the southeast of Brazil

“…(13) LBV representatives have been found to infect L. martiniquensis, of the subgenus Mundinia, in Martinique and Brazil. (14,15) Despite recent advancements in the field, the extent to which the virus affects parasite virulence or host immune responses during Leishmania infection remains unclear. In the case of LRVs, it is known that the endosymbiotic virus confers an adaptive advantage to the parasite by suppressing anti-leishmanial immunity in the vertebrate host, thereby facilitating the parasite's survival.…”

“… 13 LBV representatives have been found to infect L. martiniquensis , of the subgenus Mundinia , in Martinique and Brazil. 14 , 15 …”