First Report of Lethal Yellowing Disease of Coconut Palms Caused by Phytoplasma on Nevis Island

Myrie, Wayne; Paulraj, Litta; Dollet, Michel; Wray, Debbian; Been, B. O.; McLaughlin, Wayne

doi:10.1094/pd-90-0834a

Cited by 13 publications

(9 citation statements)

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“…La détection de ces phytoplasmes est une première étape qui doit être coordonnée avec l'étiologie et avec leur transmission. Nous recommandons que la microscopie électronique soit suivie par la sérologie et l'amplification moléculaire en chaine ou polymerase chain reaction (PCR) (Cousin et Boudon-Padieu, 2001 ;Myrie et al, 2006 ;Brown et al, 2008 ;Nejat et al, 2009) Mariau et al (1996) et Bonnot et al (2010) ont montré que celle-ci peut faire des bonds de 10, 70, et même 450 km. Certainement, le microclimat de l'île où se trouve la plantation de la SICOR s'y prête mieux à son épanouissement qu' Assinie ou ailleurs.…”

Section: Discussionunclassified

Association d’organismes de type mycoplasmes avec le dépérissement mortel des cocotiers de Grand-Lahou en Côte d’Ivoire

Amenan¹,

Atcham²,

Pohe³

et al. 2012

Int. J. Bio. Chem. Sci

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Section: Discussionunclassified

Association d’organismes de type mycoplasmes avec le dépérissement mortel des cocotiers de Grand-Lahou en Côte d’Ivoire

Amenan¹,

Atcham²,

Pohe³

et al. 2012

Int. J. Bio. Chem. Sci

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“…Two explanations have been put forward for this other leap by LY: either it was a similar process to that assumed for Mexico, or it was by the passive transportation of infectious vectors on the winds of a cyclone. The latest focus, the most unexpected, occurred this time east of the original core, on the island of Nevis during 2005-2006 [11]. The strain of the LY etiological agent on Nevis is very similar to that in Florida, and the hypothesis of a scenario identical to that at Cancun seems to be the most plausible.…”

Section: Caribbean -Americamentioning

confidence: 90%

Review of Coconut “Lethal Yellowing” type diseases Diversity, variability and diagnosis

Dollet

Quaicoe

Pilet

2009

OCL

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Coconut palms (Cocos nucifera L.) can be affected by several types of Lethal Yellowing (LY) diseases worldwide. Some of the syndromes are caused by phytoplasmas, small bacteria that are impossible to detect by light microscopy. Amplification of a given gene of the phytoplasmas by polymerase chain reaction (PCR) is the most convenient diagnosis method. The problem is that there are at least 28 "groups" of phytoplasmas and only one pair of primers-P1/P7-commonly used for PCR. As these primers belong to a very conserved gene, false positives are frequent. Consequently, alternative primers specific to one "strain" (or subgroup) have to be used, such as LY-F/LY-R for the Caribbean LY, Rohde primers for LD Tanzania. Such specific primers are sometimes restrictive. Indeed, there is variability within each strain and the sequence of the primers has to be adapted to that variability. There are at least five LY subgroups. The subgroups can only be identified by restriction fragment length polymorphism or sequencing. In Africa, two subgroups of LY phytoplasmas have been identified so far.

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“…Strain fitoplasma yang telah diketahui menyebabkan kerugian yang sangat besar pada tanaman kelapa di daerah Amerika dan Afrika adalah ca. Phytoplasma palmae, penyebab penyakit lethal yellowing (Tymon et al, 1998;Harrison et al, 2002;Myrie et al, 2006). Strain fitoplasma terakhir masuk dalam kelas A1 Karantina Tumbuhan Indonesia karena selain tanaman kelapa, strain ini juga diduga dapat menyerang tanaman kelapa sawit yang saat ini menjadi sumber devisa non migas utama di Indonesia.…”

Section: Pendahuluanunclassified

Karakterisasi Fitoplasma Penyebab Penyakit Layu Kelapa Di Pulau Derawan Menggunakan RFLP in Silico

Prasetyo

Mutaqin²,

Giyanto³

2018

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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Characterization of phytoplasmas associated with coconut wilt disease in Derawan Island using in silico RFLP. Coconut wilt disease has been reported in Derawan Island that resulted in eradication up to 10% of the total cultivated palms. The objective of this study was to detect and characterize phytoplasmas associated with coconut wilt disease in Derawan island using nested PCR technique and in silico RFLP based on 16S rRNA gene sequences. Detection of phytoplasmas was performed using nested PCR method, cloning of nPCR products, sequencing, and analysis of sequencing results using in silico RFLP. The results revealed that phytoplasmas could not be detected by PCR using P1/P7 primer pairs however it could be amplified by nested PCR using R16F2n/R16R2 primer pairs resulting amplicon at about 1.25 kb. In silico RFLP analysis indicated that phytoplasmas associated with coconut wilt disease in Derawan Island belong to 16SrII (witches broom phytoplasma). PCR product of the nPCR need to be sequenced because the R16F2n/R16R2 primer will also amplify the other bacterial species, mainly from Gram positive bacteria.Key words: coconut wilt disease, phytoplasmas, nested PCR, sequencing ABSTRAK Karakterisasi fitoplasma penyebab penyakit layu kelapa di Pulau Derawan menggunakan RFLP in silico. Penyakit layu kelapa telah ditemukan di Pulau Derawan, Kalimantan Timur yang mengakibatkan 10% tanaman dieradikasi. Penelitian ini bertujuan untuk mendeteksi dan mengkarakterisasi fitoplasma yang berasosiasi dengan penyakit layu kelapa di Pulau Derawan. Kegiatan penelitian meliputi pengambilan sampel, ekstraksi DNA, deteksi fitoplasma menggunakan teknik nested-PCR (nPCR), kloning dan sekuensing, serta analisis hasil sekuensing menggunakan RFLP in silico. Hasil penelitian menunjukkan bahwa fitoplasma tidak terdeteksi menggunakan PCR dengan primer P1/P7 tetapi menjadi terdeteksi dengan nPCR menggunakan primer R16F2n/R16R2 yang memiliki amplikon DNA sekitar 1,25 kb. Hasil analisis sekuensing menggunakan RFLP in silico membuktikan bahwa fitoplasma yang berasosiasi dengan penyakit layu kelapa di Pulau Derawan tergolong ke dalam kelompok 16SrII (witches broom phytoplasma). Deteksi fitoplasma memerlukan sekuensing karena primer R16F2n/R16R2 masih dapat mengamplifikasi beberapa bakteri Gram Positif. Kata kunci: penyakit layu kelapa, fitoplasma, nested-PCR, sekuensing PENDAHULUANKeberadaan tanaman kelapa di Pulau Derawan, Kalimantan Timur, Indonesia mulai berkurang akibat adanya penyakit layu kelapa. Padahal, tanaman ini menjadi satu-satunya tanaman perkebunan yang dibudidayakan. Selain sebagai sumber pendapatan tambahan, tanaman kelapa di pulau ini juga memiliki fungsi estetika yakni menambah kecantikan pandang pantai di pulau yang menjadi maskot pariwisata di provinsi ini. Terlebih lagi, tanaman kelapa di Indonesia menjadi komoditi sosial kedua setelah padi dengan luas areal sekitar 3,7 juta ha atau setara dengan sepertiga luas kelapa dunia yang menjadikan Indonesia sebagai negara produsen kelapa terluas di dunia (Anonim, 2007). Kejadia...

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First Report of Lethal Yellowing Disease of Coconut Palms Caused by Phytoplasma on Nevis Island

Cited by 13 publications

References 0 publications

Association d’organismes de type mycoplasmes avec le dépérissement mortel des cocotiers de Grand-Lahou en Côte d’Ivoire

Association d’organismes de type mycoplasmes avec le dépérissement mortel des cocotiers de Grand-Lahou en Côte d’Ivoire

Review of Coconut “Lethal Yellowing” type diseases Diversity, variability and diagnosis

Karakterisasi Fitoplasma Penyebab Penyakit Layu Kelapa Di Pulau Derawan Menggunakan RFLP in Silico

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