First report of Macrophomina pseudophaseolina infecting Lens culinaris

Abstract

In 2019 and 2020, charcoal rot symptoms were observed on lentil plants in fields located in the Northwest of Algeria (Mascara Tiaret and Relizane). Based on morphological and molecular identification using the internal transcribed spacer (ITS) sequence analyses, two representative isolates were identified as Macrophomina pseudophaseolina. Pathogenicity tests were performed and Koch's postulates were verified and fulfilled. To our knowledge, this is the first report of Macrophomina pseudophaseolina as the causal agent of charcoal rot of lentil in the world.

PRIMER REPORTE DE Macrophomina pseudophaseolina ASOCIADO A LA PUDRICIÓN DE RAÍZ EN CHILE APAXTLECO NATIVO (Capsicum annuum L.) EN GUERRERO, MÉXICO

“…Por otra parte, el producto obtenido por la amplificación por PCR, con los oligonucleótidos específicos para el hongo de la región del espaciador transcrito interno (ITS), presentó un tamaño de 519 pb identificándose como M. pseudophaseolina, secuencia que se depositó en el Banco de Genes (Genbank) del Centro Nacional de Información Biotecnológica (accesión KX757770.1 CSAEGro-chADMF), del mismo modo varios autores confirmaron a éste patógeno usando los oligonucleótidos ITS1/ITS4 (Brito et al, 2019;Kouadri et al, 2021).…”

Leguminous Seedborne Pathogens: Seed Health and Sustainable Crop Management

“…Infections with M. phaseolina have been reported for several legumes, including common bean, cowpea, chickpea, pea, soybean, lentil, and alfalfa [ 17 , 40 , 45 , 47 , 48 , 49 ]. Interestingly, in 2021, a report of charcoal rot in lentil caused by M. pseudophaseolina Crous, Sarr & Ndiaye was described for the first time worldwide [ 50 ]. Several studies have been carried out to find correlations among M. phaseolina isolates based on the pathogenicity, host, or geographical origin, but, in most cases, the results have been found to be fruitless [ 40 , 51 ].…”