First report of methicillin-resistant<i>Staphylococcus aureus</i>Cordobes/Chilean clone involved in nosocomial infections in Brazil

Becker, Ana Paula; Santos, O.; Castrucci, Fernanda Marques da Silva; Dias, Cícero; d’Azevedo, Pedro Alves

doi:10.1017/s095026881100210x

Cited by 25 publications

(20 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, the prevalence of SCCmec types varied between different regions [3,[25][26][27][28][29][30][31]. The Brazilian epidemic clone (BEC) carrying the SCCmec type III has been the predominant clone in several hospitals in Brazil [28,29,32]. However, in this study, a prevalence of SCCmec type II was observed, as in Caiaffa- Filho et al (2013) [30], who detected a high prevalence of this SCCmec type in a tertiary care teaching hospital in São Paulo, Brazil.…”

Section: Discussioncontrasting

confidence: 50%

See 1 more Smart Citation

Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients

Oliveira

Morey

Santos

et al. 2015

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of infections acquired in both community and hospital settings. In this study, MRSA isolated from different sources of hospitalized patients was characterized by molecular and phenotypic methods. Methodology: A total of 123 S. aureus isolates were characterized according to their genetic relatedness by repetitive element sequence based-PCR (REP-PCR), in vitro antimicrobial susceptibility profile, SCCmec typing and presence of seven virulence factor-encoding genes. Results: REP-PCR fingerprinting showed low relatedness between the isolates, and the predominance of one specific lineage or clonal group was not observed. All isolates were susceptible to teicoplanin and linezolide. All isolates were resistant to cefoxitin and penicillin, and the majority were also resistant to one or more other antimicrobials. Fifty isolates (41.7%) were intermediately resistant to vancomycin. Most isolates harbored SCCmec type II (53.7%), followed by type I (22.8%), type IV (8.1%) and type III (1.6%). All isolates harbored at least two virulence factor-encoding genes, and the prevalence was as follows: coa, 100%; icaA, 100%; hla, 13.0%; hlb, 91.1%, hld, 91.1%; lukS-PV and lukF-PV, 2.4%; and tst, 34.1%. A positive association with the presence of hla and SCCmec type II, and tst and SCCmec type I was observed. Conclusion: This study showed the high virulence potential of multidrug-resistant MRSA circulating in a teaching hospital. A high prevalence of MRSA showing intermediate vancomycin resistance was also observed, indicating the urgent need to improve strategies for controlling the use of antimicrobials for appropriate management of S. aureus infections.

show abstract

Section: Discussioncontrasting

confidence: 50%

“…These SCCmec types were also detected in MRSA surveys conducted in different Brazilian hospitals. However, the prevalence of SCCmec types varied between different regions [3,[25][26][27][28][29][30][31]. The Brazilian epidemic clone (BEC) carrying the SCCmec type III has been the predominant clone in several hospitals in Brazil [28,29,32].…”

Section: Discussionmentioning

confidence: 99%

Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients

Oliveira

Morey

Santos

et al. 2015

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…Currently, these types are commonly detected in MRSA strains associated with nosocomial infections ( Becker et al 2012 , Yamamoto et al 2012 , David et al 2013 , El-Mahdy et al 2013 ). The SCC mec type I was initially found in MRSA isolates predominantly in the UK in the 1960s.…”

mentioning

confidence: 99%

The multifaceted resources and microevolution of the successful human and animal pathogen methicillin-resistant Staphylococcus aureus

Figueiredo

Ferreira

2014

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most important bacterial pathogens based on its incidence and the severity of its associated infections. In addition, severe MRSA infections can occur in hospitalised patients or healthy individuals from the community. Studies have shown the infiltration of MRSA isolates of community origin into hospitals and variants of hospital-associated MRSA have caused infections in the community. These rapid epidemiological changes represent a challenge for the molecular characterisation of such bacteria as a hospital or community-acquired pathogen. To efficiently control the spread of MRSA, it is important to promptly detect the mecA gene, which is the determinant of methicillin resistance, using a polymerase chain reaction-based test or other rapidly and accurate methods that detect the mecA product penicillin-binding protein (PBP)2a or PBP2’. The recent emergence of MRSA isolates that harbour a mecA allotype, i.e., the mecC gene, infecting animals and humans has raised an additional and significant issue regarding MRSA laboratory detection. Antimicrobial drugs for MRSA therapy are becoming depleted and vancomycin is still the main choice in many cases. In this review, we present an overview of MRSA infections in community and healthcare settings with focus on recent changes in the global epidemiology, with special reference to the MRSA picture in Brazil.

show abstract

“…Furthermore, the analysis of DNA polymorphism performed in our study showed a low genetic similarity (45%) of strain BEC9393 with most of the strains studied. Researchers from southern Brazil analyzed isolates from a hospital in Porto Alegre and found the presence of SCCmec type I, which is related to the Cordoba/Chilean clone, warning of its possible spread, replacing BEC [44]. A study with blood strains collected from patients at the Hospital das Clínicas in São Paulo, Brazil, showed predominantly SCCmec type II [45].…”

Section: Discussionmentioning

confidence: 99%

Comparison of HRM analysis and three REP-PCR genomic fingerprint methods for rapid typing of MRSA at a Brazilian hospital

Bodnar

Martins

Oliveira

et al. 2016

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Infections caused by multidrug-resistant bacteria are increasingly common and represent a serious problem for public health. Staphylococcus aureus is one of the major agents of infections, and methicillin-resistant S. aureus (MRSA) has spread worldwide. The aim of this study was to phenotypically and genotypically characterize 55 MRSAs isolated in the University Hospital of Londrina, Paraná, Brazil, during 2010. Methodology: Bacterial isolates were characterized based on their antimicrobial susceptibility profile, biofilm production capacity, and staphylococcal chromosome cassette mec (SCCmec) type. Determination of clonal groups was performed by polymerase chain reaction using the RW3A, JB1, and BOX A1R primers and high-resolution melting (HRM) analysis. Results: The majority of isolates harbored SCCmec type II. SCCmec III, characteristic of the Brazilian endemic clone, was observed in four strains. Only two isolates harbored SCCmec type IV, which is common in community-acquired MRSA strains. Most isolates also showed resistance to more than four of the tested antimicrobials, and 30 isolates exhibited the ability to produce biofilm. DNA polymorphism analysis showed a higher discriminatory power for the JB1 primer, but RW3A revealed several clonal groups of MRSA with similar genotypic and phenotypic characteristics. HRM analysis showed eight different sequence types. Conclusions: These results are important for epidemiological studies involving MRSA infections.

show abstract

First report of methicillin-resistantStaphylococcus aureusCordobes/Chilean clone involved in nosocomial infections in Brazil

Cited by 25 publications

References 20 publications

Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients

Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients

The multifaceted resources and microevolution of the successful human and animal pathogen methicillin-resistant Staphylococcus aureus

Comparison of HRM analysis and three REP-PCR genomic fingerprint methods for rapid typing of MRSA at a Brazilian hospital

Contact Info

Product

Resources

About