First report of methicillin-resistant Staphylococcus aureus carrying the mecC gene in human samples from Iran: prevalence and molecular characteristics

Goudarzi, Mehdi; Navidinia, Masoumeh; Dadashi, Masoud; Hashemi, Ali; Pouriran, Ramin

doi:10.1016/j.nmni.2020.100832

Cited by 5 publications

(7 citation statements)

References 29 publications

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“…In addition, Dermota et al in Slovenia reported a prevalence of 1.5% for this gene in MRSA isolates possessing the mecC gene belonging to CC/ST130 (28). In our earlier research, CC/ST599 was reported as a mecC-positive S. aureus isolate (4).…”

Section: Discussionmentioning

confidence: 59%

“…As methicillin use increases, methicillin-resistant S. aureus (MRSA) is increasingly reported. Methicillin resistance is mediated by mecA and much less by mecC (4). Up to now, several molecular typing methods have been employed for genotyping S. aureus strains, including pulsed-field gel electrophoresis, staphylococcal cassette chromosome mec (SCCmec) typing, agr typing, protein A gene (spa) typing, multilocus sequence typing (MLST), and coagulase gene (coa) typing (4,5).…”

Section: Introductionmentioning

confidence: 99%

“…Methicillin resistance is mediated by mecA and much less by mecC (4). Up to now, several molecular typing methods have been employed for genotyping S. aureus strains, including pulsed-field gel electrophoresis, staphylococcal cassette chromosome mec (SCCmec) typing, agr typing, protein A gene (spa) typing, multilocus sequence typing (MLST), and coagulase gene (coa) typing (4,5). The coa typing is a multiplex polymerase chain reaction (PCR)-based method that is cost-effective, rapid, easily interpretable, and appropriate for identifying the genetic relationships among S. aureus isolates (5).…”

Section: Introductionmentioning

confidence: 99%

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First Report of the Emergence of mecC Gene and CC8/ST239 Tigecycline-Resistant Staphylococcus aureus Clonal Lineage Isolated from Chronic Suppurative Otitis Media

Amini,

Rahmani,

Hosseini

et al. 2023

Arch Clin Infect Dis

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Background: Staphylococcus aureus is one of the most significant bacteria involved in ear infections. However, insights into the molecular attributes of S. aureus collected from patients with chronic otitis media have yet to be reported in Iran. Objectives: The objective of this study was to assess the molecular characteristics of S. aureus isolated from patients with chronic otitis media. Methods: A total of 55 S. aureus strains retrieved from patients with chronic otitis media were analyzed by the disk diffusion method and polymerase chain reaction (PCR) to identify the nucA gene. Isolates were genetically classified using the coagulase typing method. S. aureus protein A (spa) typing, staphylococcal cassette chromosome mec (SCCmec) typing, and multilocus sequence typing (MLST) were performed on isolates with resistance to specific antibiotics. Results: Overall, out of 55 S. aureus isolates, resistance to mupirocin, fusidic acid, and tigecycline was identified in 12.7%, 5.4%, and 3.6% of isolates, respectively. Fusidic acid-resistant isolates belonged to ST5-SCCmecII/t002/coaII. Two tigecycline-resistant isolates belonged to CC8/ST239-SCCmecIII/t234/coaVIII. One positive mecC isolate belonged to the CC/ST130-SCCmecXI/t843/coaIII clone. Isolates with the iMLSB phenotype belonged to CC/ST80-SCCmecIV/t044/coaII (4 isolates), CC8/ST239-SCCmecIII/t388/coaVI (3 isolates), and CC8/ST8-SCCmecIV/t008/coaIII (1 isolate). Conclusions: Our results indicated that S. aureus isolated from patients with chronic otitis media possesses a unique molecular profile with a high percentage of resistance to multiple medications. These findings suggest that resuming the molecular analysis to improve the control and prevention of ear infections related to S. aureus is necessary.

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Section: Discussionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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First Report of the Emergence of mecC Gene and CC8/ST239 Tigecycline-Resistant Staphylococcus aureus Clonal Lineage Isolated from Chronic Suppurative Otitis Media

Amini,

Rahmani,

Hosseini

et al. 2023

Arch Clin Infect Dis

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“…The internationally recognized molecular biological standard for distinguishing CA-MRSA from HA-MRSA is that CA-MRSA carries CCmec IV-VI, while HA-MRSA carries SCCmec I-III [9,10]. In Iran, the United States, Europe, and most Asian countries, the distribution of SCCmec types indicated that SCCmec type III was the most prevalent, which emphasizes the nosocomial origin of these strains [11].…”

Section: Discussionmentioning

confidence: 99%

Comparison of diagnosis and treatment of MSSA and MRSA osteomyelitis in children: a case–control study of 64 patients

et al. 2023

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Background We aimed to compare the clinical characteristics of acute osteomyelitis caused by methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) in children. Methods We retrospectively analyzed the data of 64 children treated between September 2017 and June 2021. Based on the bacterial culture results, they were divided into MRSA and MSSA infection groups. Both groups were treated with debridement and vacuum-assisted closure for negative pressure drainage. Parameters including clinical manifestations, number of operations, length of hospital stay, inflammatory indicators, and concurrent arthritis were compared between the two groups. Results In the MRSA infection group, there was one case each of residual joint stiffness and pathological fracture. Conversely, the MSSA group had two cases of residual joint stiffness. The MRSA infection group was more prone to high fever (t = 3.61, P = 0.001), white blood cell count elevation (t = 2.41, P = 0.022), arthritis (X2 = 7.48, P = 0.013), metastatic abscess (X2 = 4.78, P = 0.042), and a shorter length of progression from onset to admission (t = − 2.04, P = 0.046); however, it required more surgeries (t = 2.68, P = 0.009) and longer hospital stay (t = 2.04, P = 0.045). Conclusions Pediatric acute osteomyelitis caused by MRSA is more prone to cause high fever and markedly elevated of white blood cell count, and is often accompanied with suppurative infection of adjacent joints and metastatic abscesses, thus requiring more surgeries and longer hospital stay.

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“…In the last decade, MRSA clones with a mecC gene have been detected in different animal species and humans, mainly in European countries but also on other continents, 12–18 with isolates mainly belonging to CC130, CC1943 and CC425. 1 , 5 , 19 Zoonotic transmission of mecC MRSA has been previously reported, although data on the prevalence, animal reservoir and epidemiology of mecC MRSA are still limited.…”

Section: Introductionmentioning

confidence: 99%

mecCMRSA in Israel—genomic analysis, prevalence and global perspective

Baum

Anuka

Treygerman

et al. 2022

JAC-Antimicrobial Resistance

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Background MRSA is a major global healthcare problem. In 2011, a new mec variant designated mecC was described, presenting partial identity at the DNA level, thus undetectable by routine mecA PCR. Objectives Until now, no reliable information regarding mecC MRSA prevalence was available in Israel. In this study, to the best of our knowledge, we describe the first case of mecC MRSA in Israel, with focus on genomic analysis and global context. Methods The mecC MRSA isolate was analysed by WGS with focus on phylogeny, global contextualization, virulence and resistance genes. The strain was characterized by antibiotic susceptibility testing, spa typing and presence of mecA/C and pvl genes. Results An MRSA strain (SA10610), isolated from a urine sample of an 83-year old patient, was found negative for the mecA and pvl genes. The MLST and spa type were ST130 and t1736, respectively. SA10610 presented resistance to oxacillin, penicillin and cefoxitin, and susceptibility to all non-β-lactam agents tested. Phylogenetic comparison with a global dataset of 586 mecC MRSA genomes revealed substantial genomic divergence. The nearest genomic relatives were human and animal isolates from Denmark. A screen of 12 761 S. aureus isolates collected during 2011–18 in Israel indicated this is the only mecC-positive strain. Conclusions A high degree of genetic variability was found between the SA10610 strain and previously sequenced mecC MRSA isolated worldwide. The genomic and phylogenetic analysis suggest that mecC MRSA isolates have evolved independently rather than from a common ancestor.

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First report of methicillin-resistant Staphylococcus aureus carrying the mecC gene in human samples from Iran: prevalence and molecular characteristics

Cited by 5 publications

References 29 publications

First Report of the Emergence of mecC Gene and CC8/ST239 Tigecycline-Resistant Staphylococcus aureus Clonal Lineage Isolated from Chronic Suppurative Otitis Media

First Report of the Emergence of mecC Gene and CC8/ST239 Tigecycline-Resistant Staphylococcus aureus Clonal Lineage Isolated from Chronic Suppurative Otitis Media

Comparison of diagnosis and treatment of MSSA and MRSA osteomyelitis in children: a case–control study of 64 patients

mecCMRSA in Israel—genomic analysis, prevalence and global perspective

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