First report of molecular and phylogenetic analysis of Physaloptera praeputialis in naturally infected stray cats from India

Maharana, Biswa Ranjan; Gupta, Shruti; Gupta, Surbhi; Ganguly, Anita; Kumar, Brijesh; Chandratre, Gauri A.; Bisla, R. S.

doi:10.1007/s00436-021-07163-2

Cited by 9 publications

(5 citation statements)

References 30 publications

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“…Phylogenetic analysis revealed that all species in Physalopteridae family were clustered together with strong bootstrap support, con rming the identi cation of P. clausa in hedgehogs. These ndings support earlier studies on the phylogenetic position of physalopterids among related nematodes [28][29][30][31][32].…”

Section: Discussionsupporting

confidence: 91%

First molecular and phylogenetic analysis of Physaloptera clausa parasitizing long-eared hedgehogs (Hemiechinus auritus) in Iran

Eslahi,

Pirestani,

Karami

et al. 2024

Preprint

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Purpose Physalopteridae nematodes pose a threat to a broad spectrum of animals, including amphibians, reptiles, birds, and mammals. The current study is the first molecular and phylogenetic analysis of Physaloptera clausa parasitizing long-eared hedgehogs (Hemiechinus auritus) in Iran. Methods A male road-killed H. auritus was collected from Shahriar City, Tehran province in May 2022 and it was subjected to necropsy. After isolating parasites, they underwent morphological analysis using a light microscope and based on an identification key. For molecular analysis, the genomic DNA was isolated using the FavorPrepTM Tissue Genomic DNA Extraction Mini Kit. The PCR products were sequenced, the sequence data were analyzed and multiple alignments were conducted using the Clustal Omega. For phylogenetic analysis, these nucleotide sequences were aligned in MEGA 11 based on the lowest Bayesian Information Criterion (BIC) score. Results A cluster of parasites found on the mucosa of the hedgehog's stomach. All the nematodes were identified as P. clausa. The sequence obtained in this study has been submitted to GenBank with the accession number OR088573. The phylogeny analysis revealed that the genus Physalopteraformed a major clade where P. clausa was clustered with various Physalopteraspecies, closely related to Turgida, Physalopteroides, and Skrjabinopteragenera. Conclusion Our study specified the necessity for additional taxonomic and phylogenetic research on Physalopteraspecies and related genera to fully understand the evolutionary dynamics and ecological significance of these parasites.

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Section: Discussionsupporting

confidence: 91%

First molecular and phylogenetic analysis of Physaloptera clausa parasitizing long-eared hedgehogs (Hemiechinus auritus) in Iran

Eslahi,

Pirestani,

Karami

et al. 2024

Preprint

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“…Although its occurrence here is lower than that previously reported [ 25 , 45 ], its detection confirms the circulation in southern Italy [ 83 , 84 ], which should not be overlooked since Physaloptera spp. can cause severe clinical signs in cats, including gastric erosions and marked catarrhal gastritis [ 85 , 86 ]. A massive infection by Physaloptera sp.…”

Section: Discussionmentioning

confidence: 99%

Squamata reptiles as a potential source of helminth infections when preyed on by companion animals

et al. 2023

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Background Squamate reptiles cohabiting with companion animals may represent a source of helminth infections, especially through predation by dogs and cats with an outdoor lifestyle. Methods In order to assess the role of reptiles as intermediate/paratenic hosts of trophically transmitted helminths, synanthropic reptiles (n = 245) captured from different ecological settings (i.e., households, dog shelters, urban, peri-urban and rural areas or natural parks) of southern Italy were examined for endoparasites. Parasitic cysts (i.e., larval forms of acanthocephalans, cestodes and nematodes) and free helminths (i.e., adult nematodes and digeneans) were morphologically and molecularly identified, and statistical analysis was carried out to evaluate the correlations between reptiles, infections, and ecological settings. Results Overall, 31% of reptiles were positive for at least one helminth, with Podarcis siculus (18.7%) and Tarentola mauritanica (8.1%) being the most frequently infected species. Among the parasites of medical interest, Joyeuxiella echinorhyncoides showed the highest prevalence (19.7%), followed by Diplopylidium acanthotetra (10.5%), Joyeuxiella pasqualei, Mesocestoides lineatus (5.6%) and Physaloptera sp. (3.9%). Macracanthorhynchus hirudinaceus was detected once. Podarcis siculus and T. mauritanica were associated with cestode infections. Conclusions The wide range of helminths detected here in reptiles living in sympatry with pets and the fact that many of these helminth species are parasitic and may infect companion animals (e.g., J. pasqualei, J. echinorhyncoides, D. acanthotetra, Physaloptera sp.) and humans (i.e., Macracanthorhynchushirudinaceus, Mesocestoideslineatus) indicate the potential health risk associated with pets preying on these small vertebrates. Our results indicate the need for complementary investigations of trophically transmitted parasites in dogs and cats living in sympatry with reptiles. Graphical Abstract

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“…Separately, the highest cox1 gene identity percentage for our sequence was 85.07% with a sample from Physaloptera sp. (accession number MW5146) collected from a stray cat [27], followed by Abbreviata caucasica (syn. Physaloptera mordens) (accession number: MT23195) from a chimpanzee with 81.71% similarity [28].…”

Section: Molecular Identification Of Physaloptera Spp and Phylogeneti...mentioning

confidence: 99%

Morphological and Molecular Identification of Physaloptera alata (Nematoda: Spirurida) in a Booted Eagle (Aquila pennata) from Portugal

Rentería-Solís

Ramilo

Schmäschke

et al. 2023

Animals

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Physaloptera spp. are parasitic nematodes that infect the gastrointestinal tracts of many carnivores and omnivores. Although they are distributed worldwide, Physaloptera spp. have not been studied in raptors in Portugal. In this study, we report Physaloptera alata in a booted eagle (Aquila pennata) in Portugal. Adult nematodes were discovered in the gizzard of a young booted eagle, and morphological features were consistent with those of the genus Physaloptera. DNA was extracted and a PCR assay performed to amplify a region of the 18S small subunit of the ribosomal RNA gene and the cytochrome c oxidase subunit I gene. The resulting PCR products were Sanger-sequenced, and comparison with the available sequences in the GenBank database confirmed the initial morphological classification as Physaloptera sp. Phylogenetic analysis clustered the sequence within the Physaloptera group. The presence of this parasite in raptors from Portugal is of particular importance to wildlife rehabilitation centers, disease ecologists, and wildlife professionals. Furthermore, we produced a new genetic sequence and have added it to the GenBank database of parasites in birds of prey.

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First report of molecular and phylogenetic analysis of Physaloptera praeputialis in naturally infected stray cats from India

Cited by 9 publications

References 30 publications

First molecular and phylogenetic analysis of Physaloptera clausa parasitizing long-eared hedgehogs (Hemiechinus auritus) in Iran

First molecular and phylogenetic analysis of Physaloptera clausa parasitizing long-eared hedgehogs (Hemiechinus auritus) in Iran

Squamata reptiles as a potential source of helminth infections when preyed on by companion animals

Morphological and Molecular Identification of Physaloptera alata (Nematoda: Spirurida) in a Booted Eagle (Aquila pennata) from Portugal

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