First Report of Nocardia farcinica Bursitis in a Patient with Diabetes Mellitus

Park, Soon-Deok; Kim, Hanjun; Jang, In Ho; Uh, Young; Kim, Juwon; Yoon, Kap Joon; Oh, Joo Yeon

doi:10.3343/alm.2014.34.3.252

Cited by 6 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pulmonary disease is the most common presentation of Nocardia in immunosuppressed patients and approximately one-third of affected patients have a disseminated disease ( Ambrosioni et al, 2010 ; Kandi, 2015 ; Scorey and Daniel, 2016 ). Infection of traumatic wounds produces chronic inflammation that may lead to fistulas, abscesses, cellulitis, ulcerations, and mycetoma ( Smego and Gallis, 1984 ; Salinas-Carmona, 2000 ; Salinas-Carmona et al, 2009 ), and may extend into muscles, bones, the brain, kidneys, the prostate, cornea, heart, and adjacent organs ( De Nardo et al, 2013 ; Sirijatuphat et al, 2013 ; Kumar et al, 2014 ; Park et al, 2014 ; Sharma and O’Hagan, 2016 ). Nocordia infection of the central nervous system may be acquired by cutaneous or respiratory routes ( Smego and Gallis, 1984 ; Beaman and Beaman, 1994 ; Inamadar and Palit, 2003 ; Zakaria et al, 2008 ; Chen et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Cloning, Expression, Invasion, and Immunological Reactivity of a Mammalian Cell Entry Protein Encoded by the mce1 Operon of Nocardia farcinica

Tan

Hou

et al. 2017

Front. Microbiol.

View full text Add to dashboard Cite

Bacterial mammalian cell entry (Mce) proteins have been implicated in pathogen invasion of mammalian host cells. The aim of this study was to examine the invasion-conferring ability of mce1E operon-encoded proteins, in vivo expression of Mce1E in cells from infected mice and rabbits, and Mce1E immunogenicity. Nocardia farcinica mce1E was cloned into pet30a(+) vectors, expressed in Escherichia coli, and purified. Invasion assays, transmission electron microscopy (TEM), immunoblots, and enzyme-linked immunosorbent assay (ELISA) detection of cytokines were conducted. TEM confirmed the invasion of HeLa cells by Mce1E-coated beads. The antigenicity of E. coli-expressed recombinant Mce1E was confirmed in immunoblots with sera from N. farcinica-infected mouse and rabbit sera. Co-incubation of Mce1E with splenocytes of N. farcinica-infected mice demonstrated upregulation of interferon (IFN-γ), but not interleukin (IL)-4 or IL-10, in the cultural supernatant. These findings demonstrate that Mce1E may facilitate N. farcinica interactions with and invasion of mammalian cells. Notably, Mce1E are expressed and elicited antibody responses in mice and rabbits during infection. Besides, it may play a role in cell-mediated immune reactions and cause host inflammation responses to N. farcinica infection.

show abstract

Section: Introductionmentioning

confidence: 99%

Cloning, Expression, Invasion, and Immunological Reactivity of a Mammalian Cell Entry Protein Encoded by the mce1 Operon of Nocardia farcinica

Tan

Hou

et al. 2017

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…To the best of our knowledge, cases of disseminated infection with empyema caused by N. farcinica have not been reported to date in the Republic of Korea. However, various types of localized infections caused by N. farcinica have often been reported, including catheter-related bloodstream infection, [25] brain abscess, [26] bursitis, [27] mediastinitis, [28] and soft tissue infections [29] . Although Nocardia species can infect immunocompetent hosts, [30] they are generally recognized as important opportunistic pathogens in immunocompromised hosts [31] .…”

Section: Discussionmentioning

confidence: 99%

Disseminated nocardiosis caused by Nocardia farcinica in a patient with colon cancer

et al. 2021

View full text Add to dashboard Cite

Rationale: Nocardiosis is an uncommon and potentially life-threatening infection that usually affects immunocompromised hosts. No clinical guidelines have been established for managing this rare disease, and the optimal treatment modality remains unclear. Nocardia farcinica, a relatively infrequent pathogen of nocardiosis, causes a clinically aggressive infection. In addition to our patient data, our search of the literature for patients who presented with empyema caused by N. farcinica will provide fundamental information for optimal treatment modalities.Patient concerns: A 64-year-old man was diagnosed with empyema, 4 days following surgery for sigmoid colon cancer. Brain lesions were evaluated only after N. farcinica was isolated and identified as the causative pathogen through repeated culture tests.Diagnoses: N. farcinica was isolated from the pleural effusion and confirmed as the pathogen through 16S rRNA sequencing. Interventions:The patient was successfully treated with tube thoracotomy, neurosurgical evacuation, and a combination of trimethoprim/sulfamethoxazole plus imipenem. Long-term antibiotic therapy was required to prevent recurrence.Outcomes: Pyothorax showed a good clinical response to antimicrobial therapy and drainage of pleural effusion, whereas brain abscess did not respond to medical therapy and required surgery. The patient eventually recovered and continued chemotherapy as treatment for sigmoid colon cancer.Lessons: Although extremely rare, this report demonstrates the importance of considering Nocardia infection as the differential diagnosis in immunocompromised patients who present with empyema. In particular, because of the N. farcinica infection's tendency to spread and the resistance of the organism to antibiotics, aggressive evaluation of metastatic lesions and standardized support from microbiological laboratories are important. Surgery may be required in some patients with brain abscesses to improve the chance of survival.

show abstract

“…With modern molecular techniques, such as polymerase chain reaction, restriction enzyme analysis, and 16S ribosomal RNA (rRNA) gene sequencing, faster and easier nocardia identification is possible; however, not all laboratories are equipped with these modern technologies [8]. According to some literature, sequence analysis of the 16S rRNA gene is becoming the gold standard for definitive species identification [9]. …”

Section: Discussionmentioning

confidence: 99%

A 58-Year-Old Female with Progressive Cough and Right Shoulder Pain

Thakore

Khasawneh

2016

Canadian Journal of Infectious Diseases and Medical Microbiolog

View full text Add to dashboard Cite

Cavitary pneumonia in immunocompromised patients is a challenging entity. Establishing accurate diagnosis and starting effective antibiotics are essential steps towards improving outcome. A 58-year-old stem cell transplant patient was admitted to the hospital with necrotizing pneumonia caused by nocardia. The disease progressed despite of aggrieve antimicrobial therapy. Nocardiosis continues to be a difficult disease to diagnose and treat.

show abstract

First Report of Nocardia farcinica Bursitis in a Patient with Diabetes Mellitus

Cited by 6 publications

References 13 publications

Cloning, Expression, Invasion, and Immunological Reactivity of a Mammalian Cell Entry Protein Encoded by the mce1 Operon of Nocardia farcinica

Cloning, Expression, Invasion, and Immunological Reactivity of a Mammalian Cell Entry Protein Encoded by the mce1 Operon of Nocardia farcinica

Disseminated nocardiosis caused by Nocardia farcinica in a patient with colon cancer

A 58-Year-Old Female with Progressive Cough and Right Shoulder Pain

Contact Info

Product

Resources

About