First report of porcine respirovirus 1 in South America

Agüero, Belén; Mena, Juan; Berrios, Fernanda; Tapia, Rodrigo; Salinas, Constanza; Dutta, Jayeeta; Bakel, Harm van; Mor, Sunil K.; Brito, Bárbara; Medina, Rafael; Neira, Víctor

doi:10.1016/j.vetmic.2020.108726

Cited by 20 publications

(41 citation statements)

References 16 publications

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“…In this study, SMg did not detect viruses with Ct above 30. Therefore, viral enrichment strategies to obtain near-or complete viral sequences have been applied previously, including cell culture (Lau et al, 2013;Palinski et al, 2016) followed by depletion of ribosomal background RNA (Agüero et al, 2020). BS, blood serum (pooled).…”

Section: Re Sults and Discussionmentioning

confidence: 99%

First detection of porcine respirovirus 1 in Germany and the Netherlands

Schuele

Lizarazo-Forero

Cassidy

et al. 2021

Transbound Emerg Dis

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Porcine respirovirus 1, also referred to as porcine parainfluenza virus 1 (PPIV-1), was first detected in deceased pigs from Hong Kong in 2013. It has since then been found in the USA, Chile and most recently in Hungary. Information on the pathogenicity and global spread is sparse. However, it has been speculated to play a role in the porcine respiratory disease complex. To investigate the porcine virome, we screened 53 pig samples from 26 farms within the Dutch-German border region using shotgun metagenomics sequencing (SMg). After detecting PPIV-1 in five farms through SMg, a real-time reverse transcriptase PCR (RT-qPCR) assay was designed, which not only confirmed the presence of the virus in 1 of the 5 farms but found an additional 6 positive farms. Phylogenetic analysis found the closest match to be the first detected PPIV-1 strain in Hong Kong. The Dutch-German region represents a significant area of pig farming within Europe and could provide important information on the characterization and circulation of porcine viruses, such as PPIV-1. With its recent detection in Hungary, these findings suggest widespread circulation of PPIV-1 in Central Europe, highlighting the need for further research on persistence, pathogenicity and transmission in Europe.

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Section: Re Sults and Discussionmentioning

confidence: 99%

First detection of porcine respirovirus 1 in Germany and the Netherlands

Schuele

Lizarazo-Forero

Cassidy

et al. 2021

Transbound Emerg Dis

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“…In cats, at the last sampling, serum samples were also collected. Nasal samples were collected using flocked swabs; fresh feces were stored in ziplock bags [16][17][18] and deposited in viral transport media.…”

Section: Case Description and Specimen Collectionmentioning

confidence: 99%

A household case evidences shorter shedding of SARS-CoV-2 in naturally infected cats compared to their human owners

Neira

Brito

Agüero

et al. 2021

Emerging Microbes & Infections

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been detected in domestic and wild cats. However, little is known about natural viral infections of domestic cats, although their importance for modelling disease spread, informing strategies for managing positive human-animal relationships and disease prevention. Here, we describe the SARS-CoV-2 infection in a household of two human adults and sibling cats (one male and two females) using real-time RT–PCR, an ELISA test, viral sequencing, and virus isolation. On May 5th, 2020, the cat-owners tested positive for SARS-CoV-2. Two days later, the male cat showed mild respiratory symptoms and tested positive. Four days after the male cat, the two female cats became positive, asymptomatically. Also, one human and one cat showed antibodies against SARS-CoV-2. All cats excreted detectable SARS-CoV-2 RNA for a shorter duration than humans and viral sequences analysis confirmed human-to-cat transmission. We could not determine if cat-to-cat transmission also occurred.

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“…Porcine respirovirus 1 was first identified in Hong Kong in samples from 2008 to 2012 [3] and later in the USA, Chile, Hungary, Germany, and the Netherlands [6][7][8][9]. The role of PRV1 infection for pig health is unknown.…”

Section: Introductionmentioning

confidence: 99%

Detection of Porcine Respirovirus 1 (PRV1) in Poland: Incidence of Co-Infections with Influenza A Virus (IAV) and Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in Herds with a Respiratory Disease

Woźniak

Cybulski²,

Dénes

et al. 2022

Viruses

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Porcine respirovirus 1 (PRV1) is also known as porcine parainfluenza virus 1 (PPIV1). The prevalence and the role of PRV1 infections for pig health is largely unknown. In order to assess the PRV1 prevalence in Poland, nasal swabs and oral fluids collected from pigs from 30 farms were examined with RT real-time PCR. Additionally, IAV and PRRSV infection statuses of PRV1-positive samples were examined. The results showed that the virus is highly prevalent (76.7% farms positive) and different patterns of PRV1 circulation in herds with mild–moderate respiratory disease were observed. Co-infections with IAV and PRRSV were infrequent and detected in 8 (23.5%) and 4 (11.8%) out of 34 PRV1-positive nasal swab pools from diseased pens, respectively. In one pen PRV1, IAV, and PRRSV were detected at the same time. Interestingly, PRV1 mean Ct value in samples with co-infections was significantly lower (29.8 ± 3.1) than in samples with a single PRV1 infection (32.5 ± 3.6) (p < 0.05), which suggested higher virus replication in these populations. On the other hand, the virus detection in pig populations exhibiting respiratory clinical signs, negative for PRRSV and IAV, suggests that PRV1 should be involved in differential diagnosis of respiratory problems.

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First report of porcine respirovirus 1 in South America

Cited by 20 publications

References 16 publications

First detection of porcine respirovirus 1 in Germany and the Netherlands

First detection of porcine respirovirus 1 in Germany and the Netherlands

A household case evidences shorter shedding of SARS-CoV-2 in naturally infected cats compared to their human owners

Detection of Porcine Respirovirus 1 (PRV1) in Poland: Incidence of Co-Infections with Influenza A Virus (IAV) and Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in Herds with a Respiratory Disease

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