First Report of Post-harvest Anthracnose on Mango (<i>Mangifera indica</i>) Caused by <i>Colletotrichum siamense</i> in China

Liu, L. P.; Shen, Juan; Zhang, L.; Hu, Ronggui; Chen, C. Q.; Li, Yang; Lu, B. H.; Liu, Y. N.; Yu, Li; Wang, X.; Li, Y.; Gao, Jie; Hsiang, Tom

doi:10.1094/pdis-08-16-1130-pdn

Cited by 19 publications

(8 citation statements)

References 5 publications

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“…15 , Liu et al . 24 and Qin et al . 22,25 identified four Colletotrichum species ( C. asianum , C. fructicola , C. scovillei , C. siamense ) prevalent on mango in China.…”

Section: Discussionmentioning

confidence: 94%

Colletotrichum species associated with mango in southern China

Shen

et al. 2019

Sci Rep

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Mango (Mangifera indica L.) is an economically significant fruit crop in provinces of southern China including Hainan, Yunnan, Sichuan, Guizhou, Guangdong and Fujian. The objective of this study was to examine the diversity of Colletotrichum species infecting mango cultivars in major growing areas in China, using morphological and molecular techniques together with pathogenicity tests on detached leaves and fruits. Over 200 Colletotrichum isolates were obtained across all mango orchards investigated, and 128 of them were selected for sequencing and analyses of actin (ACT), chitin synthase (CHS-1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), the internal transcribed spacer (ITS) region, β-tubulin (TUB2) genomic regions. Our results showed that the most common fungal isolates associated with mango in southern China involved 13 species: Colletotrichum asianum, C. cliviicola, C. cordylinicola, C. endophytica, C. fructicola, C. gigasporum, C. gloeosporioides, C. karstii, C. liaoningense, C. musae, C. scovillei, C. siamense and C. tropicale. The dominant species were C. asianum and C. siamense each accounting for 30%, and C. fructicola for 25%. Only C. asianum, C. fructicola, C. scovillei and C. siamense have previously been reported on mango, while the other nine Colletotrichum species listed above were first reports associated with mango in China. From this study, five Colletotrichum species, namely C. cordylinicola, C. endophytica, C. gigasporum, C. liaoningense and C. musae were the first report on mango worldwide. Pathogenicity tests revealed that all 13 species caused symptoms on artificially wounded mango fruit and leaves (cv. Tainong). There was no obvious relationship between aggressiveness and the geographic origin of the isolates. These findings will help in mango disease management and future disease resistance breeding.

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“…15 , Liu et al . 24 and Qin et al . 22,25 identified four Colletotrichum species ( C. asianum , C. fructicola , C. scovillei , C. siamense ) prevalent on mango in China.…”

Section: Discussionmentioning

confidence: 94%

Colletotrichum species associated with mango in southern China

Shen

et al. 2019

Sci Rep

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“…Anthracnose disease caused by Colletotrichum spp. has been reported to negatively impact the cultivation and production of mangoes [ 19 , 20 ], bananas [ 21 ], tomatoes [ 22 ], and chili peppers [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Biocontrol Mechanisms of Trichoderma koningiopsis PSU3-2 against Postharvest Anthracnose of Chili Pepper

Ruangwong

Pornsuriya

Pitija³

et al. 2021

JoF

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Several mechanisms are involved in the biological control of plant pathogens by the soil-borne Trichoderma spp. fungi. The aim of this study was to characterize a new strain of Trichoderma as a potential biological control agent to control the postharvest anthracnose of chili pepper caused by Colletotrichumgloeosporioides. A total of nine strains of Trichoderma spp. were screened for their antifungal activity using a dual culture assay against C.gloeosporioides. Trichoderma koningiopsis PSU3-2 was shown to be the most effective strain, with a percentage inhibition of 79.57%, which was significantly higher than that of other strains (p < 0.05). In the sealed plate method, T. koningiopsis PSU3-2 suppressed the growth of C.gloeosporioides by 38.33%. Solid-phase microextraction (SPME) was applied to trap volatiles emitted by T. koningiopsis PSU3-2, and the GC/MS profiling revealed the presence of antifungal compounds including azetidine, 2-phenylethanol, and ethyl hexadecanoate. The production of cell-wall-degrading enzymes (CWDEs) was assayed through cell-free culture filtrate (CF) of PSU3-2, and the enzyme activity of chitinase and β-1,3-glucanase was 0.06 and 0.23 U/mL, respectively, significantly higher than that in the control (p < 0.05). Scanning electron microscopy of the mycelium incubated in cell-free CF of T. koningiopsis PSU3-2 showed the abnormal shape of C.gloeosporioides hyphae. Application of T. koningiopsis PSU3-2 by the dipping method significantly reduced the lesion size (p < 0.05) after inoculation with C.gloeosporioides compared to the control, and there was no disease symptom development in T. koningiopsis PSU3-2-treated chili pepper. This study demonstrates that T. koningiopsis PSU3-2 is an effective antagonistic microorganism and a promising biocontrol agent against postharvest anthracnose of chili pepper, acting with multiple mechanisms.

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“…As such it can cause preharvest fruit anthracnose in Carica papaya L. [40], Litchi chinensis [18,41], Acca sellowiana [42], Fragaria ananassa Duch [43], Citrus spp. [44], Walnut (Juglans regia L.) [14], postharvest fruit anthracnose in Mangifera indica [45], crown rot and anthracnose in roots, runner of Fragaria ananassa Duch [43], leaf spot in rubber (Hevea brasiliensis) [37], Thai basil (Ocimum basilicum) [46], and Macadamia (Macadamia integrifolia) [27,30]. Recently Kumar et al [47] and Salunkhe et al [48] also reported C. siamense as the causal organism of Cliff Banana and cotton anthracnose in the neighboring country India.…”

Section: Discussionmentioning

confidence: 99%

Colletotrichum siamense leaf blight/spot: Characterization of a newly identified disease on Cinnamomum tamala

et al. 2023

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Cinnamomum tamala (bay leaf) is widely used for culinary and medicinal purposes in South Asia. A leaf blight/spot disease was first discovered on nearly 90% of C. tamala plants with a mean severity of 48% to 74.4% in Gazipur and Bogura, Bangladesh, in 2019. The present study identified and characterized the causal organism and formulated the optimum growth conditions and effective fungicides for the chemical control of the pathogen. The characteristic symptoms on the infected leaves appeared circular to oval reddish‐brown spots with raised margins and often developed in tear‐stain patterns. Severe infection of C. tamala sapling resulted in dieback symptoms with leaf defoliation. A fungus with floccose, dense, white colonies with well‐differentiated acervuli was isolated from the infected leaves. Combined cultural, morphological, and molecular characteristics identified the pathogen as Colletotrichum siamense. Inculcating healthy leaves and 1‐year‐old saplings of C. tamala with a conidial suspension of the fungus reproduced the same symptoms observed in the bay leaf orchard. The highest mycelial growth was recorded on V‐8 Juice Agar media, while the maximum radial mycelial growth and level of sporulation of the fungus were significantly higher in incubation temperature 30°C. Fungicide trials showed that carbendazim 50 WP, azoxystrobin, mancozeb, and trifloxystrobin, either singly or in combination, successfully reduced fungal mycelial growth in vitro. Therefore, disease management strategies should be opted to halt the further spread of this issue. To our knowledge, this is the first study to document the incidence of Colletotrichum leaf blight on C. tamala in Bangladesh and even in the world.

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First Report of Post-harvest Anthracnose on Mango (Mangifera indica) Caused by Colletotrichum siamense in China

Cited by 19 publications

References 5 publications

Colletotrichum species associated with mango in southern China

Colletotrichum species associated with mango in southern China

Biocontrol Mechanisms of Trichoderma koningiopsis PSU3-2 against Postharvest Anthracnose of Chili Pepper

Colletotrichum siamense leaf blight/spot: Characterization of a newly identified disease on Cinnamomum tamala

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