First Report of Root-Knot Nematode, Meloidogyne incognita, Infecting Buckwheat, Fagopyrum esculentum, in the State of São Paulo, Brazil

Abstract

Buckwheat (Fagopyrum esculentum Moench) belongs to the Polygonaceae family and has been widely cultivated due to its high nutritional, nutraceutical, and medicinal properties. Brazil ranks seventh-largest producer, with 66,000 tons produced in 2018. Buckwheat is also valued for its adaptability as a cover crop, in grain fields of soybean (Glycine max (L.) Merr., maize (Zea mays L.), and sorghum (Sorghum bicolor (L.) Moench) (Görgen et al. 2016, Babu et al. 2018) especially in fields highly infested with plant-parasitic nematodes (PPN). PPN cause severe root damage, suppressing plant development and yield production. In October 2018, six samples of roots and soil were collected in symptomatic patches of buckwheat, in Guaíra SP (20° 19' 32"S 48° 13' 15.4"W). Samples were analyzed in the Nematology Laboratory (LabNema), UNESP, Jaboticabal, SP, BR. Plants presented symptoms of yellow leaves and galled and volume-reduced roots. Meloidogyne sp. was found, comprising 6,320 eggs and second-stage juveniles (J2s) from 10 g of root and 1,628 J2s in 100 cm³ of soil. Adult morphological characteristics, isoenzyme phenotype of esterase, and molecular analysis were performed to identify the Meloidogyne species. The perineal patterns presented high and trapezoidal dorsal arch (n=15), and the males showed a trapezoidal labial region, including a high head cap formed by a large round labial disc that is raised above the medial lips and centrally concave (n=15) (Eisenback and Hirscmann 1981). These characteristics are typical in Meloidogyne incognita (Kofoid and White, 1912) Chitwood, 1949 (Nascimento et al., 2020; Eisenback and Hirschmann 1981; Netscher and Taylor 1974). The enzymatic phenotype was performed with females (n=8), and the phenotype I1 was verified, described by Esbenshade and Triantaphyllou (1985) as typical for M. incognita. To confirm the species DNA samples were extracted from individual females (n=6) and PCR with specific primers for M. incognita (Mi-F 5′- GTGAGGATTCAGCTCCCCAG-3′ and Mi-R 5′-ACGAGGAA CATACTTCTCCGTCC-3′) and M. javanica (Treub) Chitwood 1949 (Fjav 5′-GGTGCGCGATTGAACTGAGC-3′ and Rjav 5′-CAG GCCCTTCAGTGGAACTATAC-3′) that amplify SCAR markers described by Meng et al. (2004) and Zijlstra et al. (2000), respectively, and specific primers for M. enterolobii Yang & Eisenback 1983 that amplify rDNA-IGS2 region (Me-F 5′-AACTTTTG TGAAAGTGCCGCTG-3′ and Me-R 5′-TCAGTTCAGGCAGG ATCAACC-3′) described by Long et al. (2006) were tested. A fragment of 955 pb DNA size was amplified in Mi-F/R primer, which confirmed the M. incognita identification (Meng et. al., 2004). The original population was used to execute pathogenicity test. In a greenhouse, single buckwheat seeds (cv. IPR 91 Baili) were sown in six 5L pots filled with autoclaved-soil and inoculated with 3,000 eggs and J2s per pot (n=6) and control (n=6). After 60 days, the nematodes were extracted from roots and the M. incognita was confirmed. An average of 15,738 eggs and J2s were recovered, (reproductive factor = 5.24), which confirmed buckwheat as a host to M. incognita. The inoculated plants showed symptoms as those observed in the field. No symptom or nematode was noted on the control. Meloidogyne incognita has been reported causing high damage to the F. esculentum in California (Gardner and Caswell-Chen 1994) plus several crops in Brazil (Nascimento et al., 2020). However, this is the first report of this nematode infecting buckwheat in Brazil. Given the importance of buckwheat in Brazil, with extensive use as forage, cover crop, and its nutritional properties, this report is essential to specific management measures are adopted to avoid further losses.

Dinâmica populacional da nematofauna e sua relação com doenças de solo em sistemas de sucessão de culturas com a batata (>i<Solanum tuberosum>/i< L.) e prospecção da reação de plantas de cobertura a >i<Meloidogyne ethiopica>/i< como componente no manejo integrado de fitonematoides

“…Além da resistência do trigo-mourisco a M. ethiopica, essa cobertura tem sido cultivada devido às suas propriedades nutricionais, nutracêuticas e medicinais (FERREIRA et al, 2023). O Brasil é o sétimo maior produtor mundial, com 66.000 toneladas produzidas em 2018 (FERREIRA et al, 2023).…”

“…Além da resistência do trigo-mourisco a M. ethiopica, essa cobertura tem sido cultivada devido às suas propriedades nutricionais, nutracêuticas e medicinais (FERREIRA et al, 2023). O Brasil é o sétimo maior produtor mundial, com 66.000 toneladas produzidas em 2018 (FERREIRA et al, 2023). O trigo sarraceno também é valorizado por sua adaptabilidade como cultura de cobertura, em campos de grãos e especialmente em campos altamente infestados por nematoides (FERREIRA et al, 2023).…”

“…O Brasil é o sétimo maior produtor mundial, com 66.000 toneladas produzidas em 2018 (FERREIRA et al, 2023). O trigo sarraceno também é valorizado por sua adaptabilidade como cultura de cobertura, em campos de grãos e especialmente em campos altamente infestados por nematoides (FERREIRA et al, 2023). No entanto, em áreas infestadas por M. incognita deve-se restringir o seu cultivo em função de sua suscetibilidade a essa espécie do nematoide das galhas (FERREIRA et al, 2023).…”