First report of root rot caused by <i>Phytophthora nicotianae</i> in avocado trees (<i>Persea americana</i>) in Cuba

Machado, Maylen; Collazo, Cyrelys; Peña, Marcelino Ramírez; Coto, Orlando; López, Martínez

doi:10.5197/j.2044-0588.2013.028.009

Cited by 9 publications

(3 citation statements)

References 5 publications

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“…The results showed that when the concentrations of crRNA probes and ssDNA reporter genes were 2 µM and 10 µM (Figure 4), respectively, stable results were achieved. To improve experimental efficiency and reduce the required time, different RPA reaction times (5,10,15,20,25,30, and 35 min) and different Cas12a cleavage times (5,10,15,20,25,30, and 35 min) were tested using a fixed amount of P. nicotianae gDNA template (100 ng•µL −1 ) for detection. The results showed that fluorescence could be clearly observed after 20 min (Figure 5A,C) of the RPA reaction and after 25 min (Figure 5B,D) of Cas12a cleavage.…”

Section: Optimizing the Rpa-crispr/cas12a Assay For The Detection Of ...mentioning

confidence: 99%

“…Moreover, P. nicotianae is considered the sole pathogen affecting this crop [9]. In South Africa, it is gradually replacing P. cinnamomi in eucalyptus plantations, posing a significant threat to the health of these valuable trees [10]. By detecting diseases early, we can effectively prevent and control their spread, ultimately reducing potential losses.…”

Section: Introductionmentioning

confidence: 99%

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Rapid and Efficient Molecular Detection of Phytophthora nicotianae Based on RPA-CRISPR/Cas12a

Zang,

Dai,

Liu

et al. 2024

Forests

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Phytophthora nicotianae is a global and polyphagous pathogen with a wide host range. P. nicotianae can infect Areca catechu, Durio zibethinus L., Psidium guajava L., Hevea brasiliensis, and other tree species. The pathogen is capable of inducing butt rot and affecting aerial parts, including stems, leaves, and fruits. Compared to other Phytophthora species, P. nicotianae is more adaptable to abiotic stress. In this study, recombinase polymerase amplification (RPA) in combination with the CRISPR/Cas12a system was used for the detection of P. nicotianae, and achieved rapid and efficient detection of P. nicotianae. The assay was highly specific to P. nicotianae. All 4 tested isolates of P. nicotianae yielded positive results, whereas 30 isolates belonging to 17 other Phytophthora species, 8 fungal species, and 4 Bursaphelenchus xylophilus vermicules lacked detection. Under the conditions of 37 °C, after 20 min of RPA reaction and 25 min of Cas12a cleavage, a DNA concentration as low as 10 pg·μL−1 could be detected. In addition, it detected P. nicotianae from artificially inoculated leaves of Fatsia japonica. In this study, a novel method was established for the efficient and accurate detection of P. nicotianae based on the combination of RPA and the CRISPR/Cas12a system.

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Section: Optimizing the Rpa-crispr/cas12a Assay For The Detection Of ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rapid and Efficient Molecular Detection of Phytophthora nicotianae Based on RPA-CRISPR/Cas12a

Zang,

Dai,

Liu

et al. 2024

Forests

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“…Persea americana is attacked by many diseases, some of which are root rot caused by Phytophthora palmivora (Machado et al, 2012), gummosis and root necrosis caused by Phytophthora nicotianae (Machado et al, 2013). Molecular-based technologies are the most reliable tools for characterizing microorganisms due to the fact that they deal with the genetic composition of organisms.…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of fungi associated with avocado (Persea americana Mill.) fruits

Iyanyi¹,

Ataga²,

Rotimi³

et al. 2021

Agro-Sci.

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Avocado (Persea americana Mill.) is grown for its nutritious fruit. However, the quantity and quality of these fruits are threatened by some fungal organisms which can cause health complications when it is consumed by man. DNA extraction provides a unique tool for identification of organisms. This study was conducted to isolate and identify fungal species associated with avocado fruit using both morphological and molecular techniques. Fungal species were isolated from Persea americana purchased from Choba market, Port Harcourt, Rivers State, Nigeria using Potato Dextrose Agar (PDA) as a growth medium. The morphology of isolated fungi on PDA were cotton-like blackish grey spots, white villous colonies, greyish powdery spores and black spores for isolates 1 to 4 respectively. Extraction of DNA from fungal isolates was carried out using Zymo Fungal/Bacteria DNA Miniprep Kit. PCR amplification of the ITS1-2 regions of isolates was carried out using fungal universal primer pair; ITS4 and ITS5.PCR amplification of the ITS1-2 gene sequences yielded amplicons between 537-580 base pairs. PCR products were sequenced and the sequencing result after BLAST search revealed the identity of the four fungal species as follows: Lasiodiplodia theobromae, Fusarium proliferatum, Penicillium sp. and Aspergillus niger. This study will promote the knowledge of specific fungal species associated with Persea americanna and help plant pathologists to proffer preventive and control measures to enhance fruit protection and yield quality.

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First report of Phytophthora nicotianae causing avocado root rot in Thailand

Kankavee,

Withee,

Haituk

et al. 2023

J Gen Plant Pathol

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First report of root rot caused by Phytophthora nicotianae in avocado trees (Persea americana) in Cuba

Cited by 9 publications

References 5 publications

Rapid and Efficient Molecular Detection of Phytophthora nicotianae Based on RPA-CRISPR/Cas12a

Rapid and Efficient Molecular Detection of Phytophthora nicotianae Based on RPA-CRISPR/Cas12a

Molecular identification of fungi associated with avocado (Persea americana Mill.) fruits

First report of Phytophthora nicotianae causing avocado root rot in Thailand

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