Rose Rosette Disease (RRD) is a common disease of roses, predominantly in North America. RRD is caused by the rose rosette virus (RRV), an emaravirus transmitted by the eriophyid mite Phyllocoptes fructiphilus Keifer. The RRD characteristic symptoms include deformed leaves, abnormal thorns, and witches’ brooms. Roses are important cultivated plants around the world. Most Rosa spp. and cultivated varieties such as climbers, hybrid teas, floribundas, and miniature roses are susceptible to the disease. The first RRD case was reported in California and Manitoba, Canada in the 1940s (Conners 1941; Martin 2014); since then RRD has been reported in various U.S. states (EPPO Global Database 2019), and recently in India (Chakraborty et al. 2017). Rose rosette virus detection relies on molecular assays.