First report of Scallion mosaic virus on wild Japanese garlic (Allium macrostemon) in Japan

The genetic diversity of narcissus viruses related to turnip mosaic virus blur arbitrary boundaries used to discriminate potyvirus species

“…TuMV is a member of a clearly defined lineage of potyviruses, the TuMV phylogenetic group, all of which, except TuMV, have been isolated from monocotyledonous plants [ 3 ]. The TuMV group consists of Japanese yam mosaic virus (JYMV) [ 19 – 21 ], narcissus late season yellows virus (NLSYV) [ 22 , 23 ], narcissus yellow stripe virus (NYSV) [ 23 – 27 ], scallion mosaic virus (ScaMV) [ 14 , 28 ], wild onion symptomless virus (WoSV) [ 13 ] and TuMV [ 11 , 12 , 29 ].…”

First Genome Sequence of Wild Onion Symptomless Virus, a Novel Member of Potyvirus in the Turnip Mosaic Virus Phylogenetic Group

“…Symptomless wild onion plants were collected in the front yard of the fruit farm of Iznik. The shape of the wild onion plants was very similar to that of Japanese/Chinese garlic ( Allium macrostemon ), which is known to be distributed only in East Asia ( 1 ).…”

“…One aim of our recent studies is to find new viral species within the group of viruses related to turnip mosaic virus (TuMV) ( 1 ) to assess evolutionary relationships between the viruses. TuMV, Japanese yam mosaic virus (JYMV), narcissus late season yellows virus (NLSYV), narcissus yellow stripe virus (NYSV), and scallion mosaic virus (ScaMV) are species included in the TuMV group in the potyvirus phylogeny.…”

“…The extracted RNAs from wild onion leaves were reverse transcribed, and the cDNAs were amplified using the PrimeScript II high-fidelity one-step reverse transcription-PCR (RT-PCR) kit (TaKaRa Bio, Ohtsu, Japan). RT-PCR products of approximately 2,100 bp from the nuclear inclusion b protein gene to the 3′-end poly(A) [NIb-poly(A)] region were amplified with potyvirus-specific primer pairs modified from the primers described by Zheng et al ( 7 ) and Ohshima et al ( 1 ), POTYNIbNOT4P (5′-GGGGCGGC CGCATATGGGGTGAGAGAG GTNTGYGTNGAYGAYTTYAAYAA-3′) and Tu3T9 M (5′-GGGGCGGCCGCT15-3′). The RT-PCR products were cloned into the NotI site of plasmid pZErO-2 vector.…”

First Genome Sequence of Shallot Latent Carlavirus from Allium macrostemon Bunge

“…Wild Japanese garlic ( Allium macrostemon Bunge) is the most widely distributed wild onion plant in Japan. Only scallion mosaic potyvirus has previously been reported from Japanese garlic ( 1 ). In the spring of 2013, a Japanese garlic with chlorotic striped leaves (plant no.…”