First report of strong phosphine resistance in stored grain insects in a far northern tropical region of Australia, combining conventional and genetic diagnostics

Nayak, Manoj K.; Jagadeesan, Rajeswaran; Singarayan, Virgine T.; Nath, Nisa S.; Pavic, Hervoika; Dembowski, Brock; Daglish, Gregory J.; Schlipalius, David I.; Ebert, Paul R.

doi:10.1016/j.jspr.2021.101813

Cited by 13 publications

(12 citation statements)

References 29 publications

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“…Overall, the results revealed that the populations of S. oryzae collected from Tamil Nadu, Kerala, Andhra Pradesh and Telangana survived at both the discriminating concentrations, indicating the prevalence of strong resistant populations. Similarly, the prevalence of strong resistance to phosphine in S. oryzae was reported in Australia, China, Vietnam and other parts of the world (Nayak et al, 2021;Nguyen et al, 2015;Duong and Bui, 2004), though the frequency of resistance reported varied from the present study.…”

Section: Resultssupporting

confidence: 59%

Resistance to Phosphine In Rice Weevil <i>Sitophilus oryzae</i> (L.) from South India

Kumar

Rajan

Mohankumar

2022

ije

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Rice weevil Sitophilus oryzae (L.) is one of most serious insect pests of stored grains. Management of this is thus vital and fumigation with phosphine gas is the most common method. However, overreliance on this has resulted in the development of heritable resistance in S. oryzae. The detection of frequency and distribution of phosphine resistance is crucial for the development of resistance management strategy. This study assesses the frequency, strength and distribution of phosphine resistance in S. oryzae populations from four southern states of India viz., Tamil Nadu, Kerala, Andhra Pradesh and Telangana. The bioassay results revealed that all these survived at both the discriminating concentrations of 0.04 and 0.25 mg/ l. There were no susceptible and weak resistant populations, and exhibited varied frequency of strong resistance ranging from 13.33 to 80.90%. The populations from Pudukottai (80.90%) recorded high frequency of strong resistance followed by Trichy (68.97%) and Tirunelveli (67.82%) ones; the one from Thiruvarur exhibited lowest (13.33%). The results indicated that the resistance to phosphine in S. oryzae was common and prevalence of strong resistant individuals was prevalent across the sampled regions.

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Section: Resultssupporting

confidence: 59%

Resistance to Phosphine In Rice Weevil <i>Sitophilus oryzae</i> (L.) from South India

Kumar

Rajan

Mohankumar

2022

ije

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“…The study found that markers linked to rph2 alleles significantly increased over the period, indicating movement of resistant individuals from nearby storage locations in the landscape. Further surveys using genotyping-by-sequencing methods have shown that phosphine resistance often arises independently and then spreads across large areas through movement of grain and natural dispersion [ 189 , 190 ]. Having a reference genome will assist in determining how often independent outbreaks occur and how they may be related as well as aid in characterizing the population genetics of resistance spread globally.…”

Section: Discussionmentioning

confidence: 99%

The Genome of Rhyzopertha dominica (Fab.) (Coleoptera: Bostrichidae): Adaptation for Success

Oppert

Muszewska

Steczkiewicz

et al. 2022

Genes

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The lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), is a major global pest of cereal grains. Infestations are difficult to control as larvae feed inside grain kernels, and many populations are resistant to both contact insecticides and fumigants. We sequenced the genome of R. dominica to identify genes responsible for important biological functions and develop more targeted and efficacious management strategies. The genome was assembled from long read sequencing and long-range scaffolding technologies. The genome assembly is 479.1 Mb, close to the predicted genome size of 480.4 Mb by flow cytometry. This assembly is among the most contiguous beetle assembly published to date, with 139 scaffolds, an N50 of 53.6 Mb, and L50 of 4, indicating chromosome-scale scaffolds. Predicted genes from biologically relevant groups were manually annotated using transcriptome data from adults and different larval tissues to guide annotation. The expansion of carbohydrase and serine peptidase genes suggest that they combine to enable efficient digestion of cereal proteins. A reduction in the copy number of several detoxification gene families relative to other coleopterans may reflect the low selective pressure on these genes in an insect that spends most of its life feeding internally. Chemoreceptor genes contain elevated numbers of pseudogenes for odorant receptors that also may be related to the recent ontogenetic shift of R. dominica to a diet consisting primarily of stored grains. Analysis of repetitive sequences will further define the evolution of bostrichid beetles compared to other species. The data overall contribute significantly to coleopteran genetic research.

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“…However, the inadequate application and intensive use of phosphine gas have triggered resistance in insect‐pest populations 6‐9 . Poorly sealed storage structures and phosphine sorption by grains result in insufficient dosages and contribute to selecting resistant populations 10‐12 .…”

Section: Introductionmentioning

confidence: 99%

Toxicity of allyl isothiocyanate applied in systems with or without recirculation for controllingSitophilus zeamais, Rhyzopertha dominica, andTribolium castaneumin corn grains

et al. 2023

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BACKGROUNDThe application of allyl isothiocyanate (AITC) has been proposed as an alternative to control stored‐grain insects. However, AITC is a compound with a low diffusion coefficient, making its distribution throughout the grain mass difficult. Therefore, the objective of the present study was to evaluate the effectiveness of AITC applied in systems with or without recirculation for controlling Sitophilus zeamais (Mots. 1855) (Coleoptera: Curculionidae), Rhyzopertha dominica (Fabr.) (Coleoptera: Bostrichidae), and Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) infesting a corn grain mass. The assays used a polyvinyl chloride (PVC) prototype, dimensioned 1.60 m in length, 0.30 m in diameter, and a static capacity of 60 kg of grains. AITC toxicity to insects was evaluated at the base, 0.5 m from the base, and top of the grain column (1.0 m). Different concentrations of AITC were tested for an exposure period of 48 h.RESULTSIn the system without AITC recirculation, insect mortality was verified only at the base of the grain column. However, insect mortality was considered uniform at the different positions of the column when the AITC recirculation system was adopted. In this system, there was also a marked reduction in the instantaneous population growth rate of S. zeamais, T. castaneum, and R. dominica, and a decrease in the dry matter loss of the grains, when the AITC concentrations were increased.CONCLUSIONAITC recirculation proved to be a viable strategy for protecting grains against the species S. zeamais, R. dominica, and T. castaneum. AITC fumigation ultimately did not cause changes in grain quality. © 2023 Society of Chemical Industry.

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First report of strong phosphine resistance in stored grain insects in a far northern tropical region of Australia, combining conventional and genetic diagnostics

Cited by 13 publications

References 29 publications

Resistance to Phosphine In Rice Weevil <i>Sitophilus oryzae</i> (L.) from South India

Resistance to Phosphine In Rice Weevil <i>Sitophilus oryzae</i> (L.) from South India

The Genome of Rhyzopertha dominica (Fab.) (Coleoptera: Bostrichidae): Adaptation for Success

Toxicity of allyl isothiocyanate applied in systems with or without recirculation for controllingSitophilus zeamais, Rhyzopertha dominica, andTribolium castaneumin corn grains

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