First report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-producing Klebsiella pneumoniae from Iran

Zahedani, Shahram Shahraki; Rigi, Shahnaz; Bokaeian, Mohammad; Ansari-Moghaddam, Alireza; Moghadampour, Mehdi

doi:10.1590/0037-8682-0114-2016

Cited by 8 publications

(5 citation statements)

References 17 publications

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“…To the best of our knowledge, this is the second report of bla IMP -harbored K. pneumoniae from Iran after Khorvash et al [27]. This study showed a high prevalence of ESBLs-encoding genes (bla SHV , bla CTX-M , and bla TEM ) among CRKP isolates, which is consistent with other studies conducted in Isfahan [25,26] and Iran [28][29][30]. However, we have not detected bla KPC , bla GES , bla IMI , and bla VIM among CRKP isolates.…”

Section: Discussionsupporting

confidence: 90%

High rate of carbapenem-resistant Klebsiella pneumoniae detected from hospital equipments in Iran

Moghadampour

Salari-Jazi

Faghri

2018

AMicr

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The objective of this study was to assess the prevalence, antibiogram, and related genes of carbapenem-resistant Klebsiella pneumoniae (CRKP) among hospital environment samples. A total of 250 samples were taken from different surfaces and medical devices of three hospitals in Isfahan, Iran. All samples were cultured and K. pneumoniae strains were identified by conventional microbiological methods and polymerase chain reaction (PCR). Antibiogram of isolates was performed by disk diffusion method and production of carbapenemases and metallo-β-lactamases (MBLs) was confirmed using modified Hodge test and E-test, respectively. Molecular detection of the related genes was carried out by PCR. Overall, 37 (14.8%) K. pneumoniae strains were isolated, of which 34 (91.9%) strains were resistant to carbapenems. Twenty-eight (82.4%) isolates were positive for carbapenemases and seven (20.6%) isolates were phenotypically MBL producers. The results of PCR showed that the prevalence of bla OXA-48 , bla NDM , bla IMP , bla SHV , bla CTX-M , bla TEM , and class 1 integron among CRKP isolates was 70.6%, 52.9%, 2.9%, 100%, 82.4%, 55.9%, and 76.5%, respectively. However, bla KPC , bla GES , bla IMI , bla VIM , and class 2 integron were not detected in any of the isolates. This study showed that the environment of our hospitals is contaminated with CRKP and it emphasizes the importance of using standard methods for infection control.

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Section: Discussionsupporting

confidence: 90%

High rate of carbapenem-resistant Klebsiella pneumoniae detected from hospital equipments in Iran

Moghadampour

Salari-Jazi

Faghri

2018

AMicr

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“…ESBL-producing K. pneumoniae isolates are of great concern, both in treatment and in management of nosocomial infections. As multiple resistance genes mostly reside The frequency of ESBL-producing K. pneumoniae isolates was higher in this study than in some previous Iranian studies (16)(17)(18). However, higher frequencies were reported by Mahmoudi et (19,20).…”

Section: Discussioncontrasting

confidence: 66%

High Frequency of qnr Genes in Urinary Isolates of Extended-Spectrum β-Lactamase (ESBL)-producing Klebsiella pneumoniae in Tehran, Iran

2019

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Background:The emergence and spread of plasmid-mediated quinolone resistance (PMQR) among extended-spectrum betalactamase (ESBL)-producing isolates have become a serious threat to global public health. Objectives:The purpose of this study was to determine the presence of PMQR determinants in ESBL-producing Klebsiella pneumoniae isolates from hospitalized patients. Methods: We collected 100 strains of K. pneumoniae from patients admitted to Milad Hospital. Antimicrobial susceptibility and ESBL production were tested based on the Clinical and Laboratory Standards Institute (CLSI) guidelines. Polymerase chain reaction (PCR) was used to determine the presence of ESBL and PMQR genes. The genetic relatedness among the strains was evaluated by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). Results: We found 51% (n = 51) of the isolates as ESBL producers. Among them, the highest resistance rates were to cefepime (76.5%), amikacin (74.5%), ciprofloxacin and levofloxacin (both 70.6%), and the lowest resistance rate was to ticarcillin (96%). Among the 51 ESBL-positive isolates, blaTEM (94.1%) was the most frequent gene, followed by blaSHV (66.7%) and blaCTXM (59.9%). The frequencies of aac(6')-Ib, qnrS, qnrD, qnrB, qnrA, and qnrC were 35.3%, 33.3%, 31.4%, 29.4%, 13.7%, and 5.9%, respectively. Coexistence of ESBL and PMQR genes was observed in 44 (86.2%) isolates. ERIC-PCR analysis revealed that ESBL producers were genetically divergent. Conclusions: Our findings indicate that PMQR genes are highly prevalent in ESBL-producing strains of K. pneumoniae in Iran. Regular detection of ESBL strains and monitoring of their antibiotic resistance patterns may help reduce the spread of these strains in hospitals.

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“…In addition, the present study demonstrated that the blaTEM type β-lactamase gene was the most prevalent ESBL gene in UPEC, which is consistent with the findings of several previously published studies (27)(28)(29)(30). In Italy (45.4%) [31], Portugal (40.9%) [32], and Turkey (72.7%), the blaTEM type βlactamase gene was the most prevalent [33].…”

Section: Discussionsupporting

confidence: 92%

Molecular detection of blaTEM, blaSHV,and blaCTX-M genes among Uropathogenic Escherichia coli isolated from cases with urinary tract infection in Erbil city-Iraq

K Ahmed,

A Hawezy

2023

DJM

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Background: Urinary tract infections (UTIs) are the most popular type of diagnosed bacterial illness, and the most frequent cause of bacteria responsible for UTIs is Escherichia coli (E. coli). β-lactamases are the most frequent resistance for gram-negative bacteria to beta-lactam antibiotics, especially in E. coli. The number of patients infected by extended-spectrum β-lactamases (ESBLs) producing E. coli was rising and regarded as a significant global health problem. Objective: evaluate how frequently blaTEM, blaSHV, and blaCTX-M genes were detected in E. coli isolated from UTIs. Patients and Methods: We collected 54 midstream urine samples from patients with symptomatic UTIs, in all age groups, from the outpatient department in Erbil hospitals from October 1, 2021 to April 1, 2022 for the isolation of E. coli. All samples were analyzed for the detection of blaTEM, blaSHV, and blaCTX genes using the polymerase chain reaction (PCR) method. Results: Most of the samples were taken from females (61.11%); according to their ages, they were divided into two groups, and most of the samples (74.07%) were taken from patients below 40 years old. PCR testing for all ESBL-producing E. coli isolate samples revealed that 16S rRNA 797 was the most frequently detected gene in all analyzed samples (100%), while it was less frequently detected in blaCTX 585 (48.15%). Conclusion: Colonization with S. aureus and MRSA inversely correlated with younger This found that elevated ESBL genes in E. coli isolated from symptomatic UTIs in our community increase the risk of possible resistance. Keywords: Uropathogenic Escherichia coli, Urinary tract infections, blaTEM, blaSHV, blaCTX-M, PCR assay

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First report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-producing Klebsiella pneumoniae from Iran

Cited by 8 publications

References 17 publications

High rate of carbapenem-resistant Klebsiella pneumoniae detected from hospital equipments in Iran

High rate of carbapenem-resistant Klebsiella pneumoniae detected from hospital equipments in Iran

High Frequency of qnr Genes in Urinary Isolates of Extended-Spectrum β-Lactamase (ESBL)-producing Klebsiella pneumoniae in Tehran, Iran

Molecular detection of blaTEM, blaSHV,and blaCTX-M genes among Uropathogenic Escherichia coli isolated from cases with urinary tract infection in Erbil city-Iraq

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