First Report of the Colistin Resistance Gene
            <i>mcr-10.1</i>
            Carried by Inc
            <sub>pA1763-KPC</sub>
            Plasmid pSL12517-mcr10.1 in Enterobacter cloacae in Sierra Leone

Guan, Jiayao; Li, Letian; Zheng, Lin; Lu, Gejin; Wang, Ying; Lakoh, Sulaiman; Sevalie, Stephen; Jiang, Bowen; Ji, Xue; Sun, Yang; Li, Jun; Zhu, Lingwei; Guo, Xuejun

doi:10.1128/spectrum.01127-22

Cited by 11 publications

(11 citation statements)

References 42 publications

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“…For instance, Wang et al [ 8 ] obtained a colistin-resistant E. roggenkampi strain when transferring mcr-10 from a susceptible strain by cloning. Expression data by Guan et al [ 18 ] and Xu et al [ 21 ] further suggest that other, chromosomic genes might mediate the high level of colistin resistance observed in their Enterobacter spp. isolates.…”

Section: Discussionmentioning

confidence: 98%

“…This specific colistin resistance gene has been so far isolated mostly in Enterobacter spp. [ 8 , 17 , 18 ], but was described also in Klebsiella spp. [ 14 , 19 ] and in E. coli [ 20 ], showing, like other mcr genes, the potential for a wide host range.…”

Section: Discussionmentioning

confidence: 99%

“…Finally, despite carrying the mcr gene, the three K. quasipneumoniae isolates were all susceptible to colistin by MIC. Several studies reported a lack of phenotypical resistance to colistin in mcr-10 -positive bacteria [ 8 , 17 , 19 ], but other authors found high levels of resistance [ 18 , 20 , 21 ], suggesting that mcr-10 expression is largely dependent on the genetic context of the gene. For instance, Wang et al [ 8 ] obtained a colistin-resistant E. roggenkampi strain when transferring mcr-10 from a susceptible strain by cloning.…”

Section: Discussionmentioning

confidence: 99%

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Wastewater-based surveillance in Italy leading to the first detection of mcr-10-positive Klebsiella quasipneumoniae

Formenti

Guarneri

Bertasio

et al. 2022

Antimicrob Resist Infect Control

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Wastewater-based surveillance enabled the first detection of the mobile colistin resistance gene mcr-10 in Italy. This plasmid-borne resistance gene was found in strains of Klebsiella quasipneumoniae isolated from samples of human raw sewage collected over several months. Although the isolates were phenotypically susceptible to colistin, the emergence of mcr-10 is concerning due to the highly variable expression of the gene and the potential for horizontal transfer to other species. In addition, the strains also carried an extended-spectrum β-lactamase gene and were phenotypically resistant to several beta-lactams. This study highlights the value of wastewater-based surveillance as an effective tool to monitor the emergence of antimicrobial resistance in strains circulating in the community and the environment.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Wastewater-based surveillance in Italy leading to the first detection of mcr-10-positive Klebsiella quasipneumoniae

Formenti

Guarneri

Bertasio

et al. 2022

Antimicrob Resist Infect Control

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“…E. coli [ 49 ], S. enterica [ 13 ], ECC [ 17 ], and K. pneumoniae [ 50 ]. In contrast, mcr-10 was almost hosted in ECC and sporadically isolated from environment [ 16 ], animal [ 51 ] and clinical samples [ 52 ]. Previous studies have shown that mcr-9 and mcr-10 were frequently carried in ECC by using single centre or endemic data [ 14 , 16 , 22 , 23 ].…”

Section: Discussionmentioning

confidence: 99%

Frequent convergence of mcr-9 and carbapenemase genes in Enterobacter cloacae complex driven by epidemic plasmids and host incompatibility

Xue

Chen

et al. 2022

Emerging Microbes & Infections

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Convergence of mcr and carbapenemase genes has been sporadically detected in Enterobacter cloacae complex (ECC) with an upward trend. However, the state of the epidemic and underlying mechanism of such convergence has been poorly understood. In this study, the co-occurrence of MCR and carbapenemases was systematically analyzed in 230 clinical ECC isolates collected between 2000 and 2018 together with a global dataset consisting of 3,559 ECC genomes compiled from GenBank. We identified 48 mcr-9 / mcr-10 -positive isolates (MCR-ECC) (20.9%) in our collection, and a comparable ratio of MCR-ECC (720/3559, 20.2%) was detected in the global dataset. A high prevalence of carbapenemase-producing MCR-ECC (MCR-CREC) was further identified in the MCR-ECC of both datasets (16/48, 33.3%; 388/720, 53.9%), demonstrating a frequent convergence of mcr-9/10 and carbapenemase genes in ECC worldwide. An epidemic IncHI2/2A plasmid with a highly conserved backbone was identified and largely contributed to the dissemination of mcr-9 in ECC worldwide. A highly conserved IncX3-type NDM-1-carrying plasmid and IncN-type IMP-4-carrying plasmid were additionally detected in MCR-CREC isolated in China. Our surveillance data showed that MCR-CREC emerged (in 2013) much later than MCR-ECC (in 2000), indicating that MCR-CREC could be derived from MCR-ECC by additional captures of carbapenemase-encoding plasmids. Tests of plasmid stability and incompatibility showed that the mcr-9/mcr-10 -encoding plasmids with the NDM-1-encoding plasmids stably remained in ECC but incompatible in Escherichia coli , suggesting that the convergence was host-dependent. The findings extend our concern on the convergence of resistance to the last resort antibiotics and highlight the necessity of continued surveillance in the future.

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“…Ten mcr homologues ( mcr -1–10) have so far been identified, and mcr-1 continues to be the main character and is widely distributed [ 10 , 11 , 12 ]. The MIC of colistin in E. coli have been shown to increase by 4 to 8-fold when the mcr-1 gene is present, indicating that mcr-1 alone can provide adequate resistance against colistin in E. coli and other Enterobacteriaceae [ 2 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Genomic Insights into the Colistin Resistant mcr-Carrying Escherichia coli Strains in a Tertiary Hospital in China

et al. 2022

Antibiotics

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Colistin is an important antimicrobial agent in the treatment of infections caused by multidrug resistant (MDR) Gram-negative bacteria. The horizontal transfer of mobile colistin resistance gene (mcr) poses a major threat to the public health worldwide. In this study, a total of thirteen mcr-carrying Escherichia coli (MCREC) strains were recovered from a tertiary hospital in Zhejiang, China, between 2016 and 2019. The minimum inhibitory concentration (MIC) of antimicrobial agents, epidemiological characteristics, and transmission dynamics of mcr-carrying isolates were analyzed using antimicrobial susceptibility testing, whole-genome sequencing, S1 nuclease pulsed-field gel electrophoresis (S1-PFGE), and southern blotting analysis. All strains were discovered to be resistant to colistin, and the majority displayed MDR phenotype. However, none of the 13 MCREC strains were resistant to carbapenems. The 13 MCREC isolates were divided into 10 different STs, including ST744, ST156, ST453, ST410, ST57, ST131, ST7034, ST2599, ST457, and ST13239, in which ST13239 was discovered for the first time. Based on core genome single nucleotide polymorphism (cgSNP) analysis, no clear epidemiological link was discovered in these strains with the exception of EC2118 and EC3807, which differ by just one SNP. A total of 35 antimicrobial resistance genes which can be divided into 14 classes were identified from the 13 MCREC isolates. According to S1-PFGE and southern blotting analyses, all 13 MCREC strains had plasmid-mediated mcr-1, and nine of them carried conjugative plasmids. In conclusion, our study revealed the emergence and dissemination of colistin-resistant E. coli isolates carrying mcr-1 in a Chinese hospital, which poses a potential risk to anti-infective therapy. More efforts should be taken to monitor the prevalence of mcr-1-carrying bacteria in China.

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First Report of the Colistin Resistance Gene mcr-10.1 Carried by Inc _pA1763-KPC Plasmid pSL12517-mcr10.1 in Enterobacter cloacae in Sierra Leone

Cited by 11 publications

References 42 publications

Wastewater-based surveillance in Italy leading to the first detection of mcr-10-positive Klebsiella quasipneumoniae

Wastewater-based surveillance in Italy leading to the first detection of mcr-10-positive Klebsiella quasipneumoniae

Frequent convergence of mcr-9 and carbapenemase genes in Enterobacter cloacae complex driven by epidemic plasmids and host incompatibility

Genomic Insights into the Colistin Resistant mcr-Carrying Escherichia coli Strains in a Tertiary Hospital in China

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First Report of the Colistin Resistance Gene mcr-10.1 Carried by Inc pA1763-KPC Plasmid pSL12517-mcr10.1 in Enterobacter cloacae in Sierra Leone

Cited by 11 publications

References 42 publications

Wastewater-based surveillance in Italy leading to the first detection of mcr-10-positive Klebsiella quasipneumoniae

Wastewater-based surveillance in Italy leading to the first detection of mcr-10-positive Klebsiella quasipneumoniae

Frequent convergence of mcr-9 and carbapenemase genes in Enterobacter cloacae complex driven by epidemic plasmids and host incompatibility

Genomic Insights into the Colistin Resistant mcr-Carrying Escherichia coli Strains in a Tertiary Hospital in China

Contact Info

Product

Resources

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First Report of the Colistin Resistance Gene mcr-10.1 Carried by Inc _pA1763-KPC Plasmid pSL12517-mcr10.1 in Enterobacter cloacae in Sierra Leone