“…Genes estA (ETEC thermo stable toxin), ehxA (pO157 marker), toxB (pO157 marker), katP (pO157 marker), espP (pO157 marker), iha (OI-48) efa-1 (OI-122 marker), nleB (OI-122 marker), nleE (OI-122 marker), sen (OI-122 marker), pagC (OI-122 marker), etpD (pO157 marker), cdt-V (cytolethal distending toxin -V), astA (EAST-1 toxin) subAB (pO113 marker), saa (pO113 marker), sab (STEC pO113 marker) and hes (pO113 marker) were searched by conventional PCR, while genes Z2098 (OI-57 marker), Z2099 (OI-57 marker), Z2121 (OI-57 marker), espK (OI-50 marker), ureD (OI-43/48 marker), espM1 (OI-71 marker), espN (OI-50 marker), terE (chromosomal gene associated with tellurite resistance) and espV (OI-44 marker) were investigated by real-time PCR. Primers and probes employed in these assays were as previously described [29,[35][36][37][38][39][40][41][42].…”