First Report of Tomato Brown Rugose Fruit Virus Infecting Tomato in Greece

Beris, Despoina; Malandraki, Ioanna; Kektsidou, Oxana; Theologidis, Ioannis; Vassilakos, Nikon; Varveri, C.

doi:10.1094/pdis-01-20-0212-pdn

Cited by 44 publications

(26 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tomato plants in this greenhouse, carrying the Tm-2 2 resistance gene, effective against ToMV, displayed disease symptoms that included a heavy mosaic pattern on leaves, narrowing of leaves, and yellow spotted fruit, causing heavy losses to fruit yield and quality. Within a short period of time, the new virus spread globally: during 2018 it was identified in tomato plants grown in Mexico, USA, Germany, Italy, and the Palestinian authority [ 19 , 20 , 21 , 22 , 23 ], and recently in Turkey, China, Greece, Egypt, and Spain [ 24 , 25 , 26 , 27 , 28 ]. This very rapid spread demonstrates that ToBRFV has become a worldwide threat to tomato production.…”

Section: Introductionmentioning

confidence: 99%

Identification and Mapping of Tomato Genome Loci Controlling Tolerance and Resistance to Tomato Brown Rugose Fruit Virus

Zinger

Lapidot

Harel

et al. 2021

Plants

View full text Add to dashboard Cite

Tomato brown rugose fruit virus (ToBRFV) was identified in Israel during October 2014 in tomato plants (Solanum lycopersicum). These plants, carrying the durable resistance gene against tomato mosaic virus, Tm-22, displayed severe disease symptoms and losses to fruit yield and quality. These plants were found infected with a tobamovirus similar to that discovered earlier in Jordan. This study was designed to screen and identify tomato genotypes resistant or tolerant to ToBRFV. The identified resistance and tolerance traits were further characterized virologically and genetically. Finally, DNA markers linked to genes controlling these traits were developed as tools to expedite resistance breeding. To achieve these objectives, 160 genotypes were screened, resulting in the identification of an unexpectedly high number of tolerant genotypes and a single genotype resistant to the virus. A selected tolerant genotype and the resistant genotype were further analyzed. Analysis of genetic inheritance revealed that a single recessive gene controls tolerance whereas at least two genes control resistance. Allelic test between the tolerant and the resistant genotype revealed that these two genotypes share a locus controlling tolerance, mapped to chromosome 11. This locus displayed a strong association with the tolerance trait, explaining nearly 91% of its variation in segregating populations. This same locus displayed a statistically significant association with symptom levels in segregating populations based on the resistant genotype. However, in these populations, the locus was able to explain only ~41% of the variation in symptom levels, confirming that additional loci are involved in the genetic control of the resistance trait in this genotype. A locus on chromosome 2, at the region of the Tm-1 gene, was finally found to interact with the locus discovered on chromosome 11 to control resistance.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification and Mapping of Tomato Genome Loci Controlling Tolerance and Resistance to Tomato Brown Rugose Fruit Virus

Zinger

Lapidot

Harel

et al. 2021

Plants

View full text Add to dashboard Cite

show abstract

“…ToBRFV was identified and described for the first time in 2016 by Salem et al [ 12 ] in tomato plants grown in a greenhouse in Jordan, and in 2017 on tomato plants harboring the Tm-2 2 gene in Israel [ 16 ]. To date, ToBRFV has been detected in tomato plants in Mexico [ 17 ], the United States of America (California) [ 18 ], Germany [ 19 ], Italy [ 3 ], Palestine [ 20 ], Turkey [ 21 ], United Kingdom [ 22 ], Greece [ 23 ], China [ 24 ], Spain [ 25 ], Holland [ 26 ], France [ 27 ], Czech Republic [ 28 ] and Cyprus [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Tomato Brown Rugose Fruit Virus: Seed Transmission Rate and Efficacy of Different Seed Disinfection Treatments

Davino

Caruso

Bertacca

et al. 2020

Plants

View full text Add to dashboard Cite

Tomato brown rugose fruit virus (ToBRFV) is a highly infectious virus, that is becoming a threat to tomato production worldwide. In this work we evaluated the localization of ToBRFV particles in tomato seeds, its seed transmission rate and efficacy of disinfection, and the effects of different thermal- and chemical-based treatments on ToBRFV-infected seeds’ germination. Analyses demonstrated that ToBRFV was located in the seed coat, sometime in the endosperm, but never in the embryo; its transmission from infected seeds to plantlets occurs by micro-lesions during the germination. The ToBRFV seed transmission rate was 2.8% in cotyledons and 1.8% in the third true leaf. Regarding the different disinfection treatments, they returned 100% of germination at 14 days post-treatment (dpt), except for the treatment with 2% hydrochloric acid +1.5% sodium hypochlorite for 24 h, for which no seed germinated after 14 dpt. All treatments have the ability to inactivate ToBRFV, but in six out of seven treatments ToBRFV was still detectable by RT-qPCR. These results raise many questions about the correct way to carry out diagnosis at customs. To our knowledge, this is the first study on the effective localization of ToBRFV particles in seeds.

show abstract

“…The tobamovirus tomato brown rugose fruit virus (ToBRFV) and the potexvirus pepino mosaic virus (PepMV) have been established as major disease-causing agents in tomatoes ( Lycopersicun esculentum ) worldwide. Recently, ToBRFV, identified in Jordan and Israel [ 1 , 2 ], has spread in the Middle East and to Europe, America and China [ 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ]. Unlike ToBRFV, PepMV worldwide spread has long been established [ 11 , 12 , 13 , 14 , 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Tomato Brown Rugose Fruit Virus Contributes to Enhanced Pepino Mosaic Virus Titers in Tomato Plants

Klap

Luria

Smith

et al. 2020

Viruses

View full text Add to dashboard Cite

The tobamovirus tomato brown rugose fruit virus (ToBRFV), a major threat to tomato production worldwide, has recently been documented in mixed infections with the potexvirus pepino mosaic virus (PepMV) CH2 strain in traded tomatoes in Israel. A study of greenhouse tomato plants in Israel revealed severe new viral disease symptoms including open unripe fruits and yellow patched leaves. PepMV was only detected in mixed infections with ToBRFV in all 104 tested sites, using serological and molecular analyses. Six PepMV isolates were identified, all had predicted amino acids characteristic of CH2 mild strains excluding an isoleucine at amino acid position 995 of the replicase. High-throughput sequencing of viral RNA extracted from four selected symptomatic plants showed solely the ToBRFV and PepMV, with total aligned read ratios of 40.61% and 11.73%, respectively, indicating prevalence of the viruses. Analyses of interactions between the co-infecting viruses by sequential and mixed viral inoculations of tomato plants, at various temperatures, showed a prominent increase in PepMV titers in ToBRFV pre-inoculated plants and in mixed-infected plants at 18–25 °C, compared to PepMV-single inoculations, as analyzed by Western blot and quantitative RT-PCR tests. These results suggest that Israeli mild PepMV isolate infections, preceded by ToBRFV, could induce symptoms characteristic of PepMV aggressive strains.

show abstract

First Report of Tomato Brown Rugose Fruit Virus Infecting Tomato in Greece

Cited by 44 publications

References 2 publications

Identification and Mapping of Tomato Genome Loci Controlling Tolerance and Resistance to Tomato Brown Rugose Fruit Virus

Identification and Mapping of Tomato Genome Loci Controlling Tolerance and Resistance to Tomato Brown Rugose Fruit Virus

Tomato Brown Rugose Fruit Virus: Seed Transmission Rate and Efficacy of Different Seed Disinfection Treatments

Tomato Brown Rugose Fruit Virus Contributes to Enhanced Pepino Mosaic Virus Titers in Tomato Plants

Contact Info

Product

Resources

About