First report of zoonotic Cryptosporidium parvum GP60 subtypes IIaA15G2R1 and IIaA16G3R1 in wild ponies from the northern Iberian Peninsula

Couso-Pérez, Seila; Limia, F Bárcena-Varela de; Ares-Mazás, Elvira; Goméz-Couso, Hipólito

doi:10.1007/s00436-019-06529-x

Cited by 11 publications

(11 citation statements)

References 25 publications

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“…It was also found in a patient with acute diarrhea in South Korea [24] and in patients from Malaysia with HIV/AIDS [20]. The second one has been reported in lambs and kids with clinical cryptosporidiosis in France [25] and in Spain [10], and more surprisingly in asymptomatic infections in wild ponies of the Iberian Peninsula [6]. This genotype has been found in children in Iran [29] and in sporadic infections in Canada [47] and Australia [50].…”

Section: Discussionmentioning

confidence: 89%

Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum

et al. 2020

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Protozoan parasites of the Cryptosporidium genus cause severe cryptosporidiosis in newborn lambs. However, asymptomatic infections also occur frequently in lambs and ewes. In sheep, the most commonly detected Cryptosporidium species are C. ubiquitum, C. xiaoi and C. parvum. Due to a lack of relevant information about such infections in France, we investigated the situation on five dairy sheep farms in the Pyrénées-Atlantiques Department in south-western France in December 2017. Individual fecal samples were collected from 79 female lambs (5–17 days old) and their mothers (72 ewes). Oocysts were screened using Heine staining before and after Bailenger concentrations. Cryptosporidium species identification and genotyping were performed using real-time PCR and gp60 gene sequencing. No cases of clinical cryptosporidiosis were observed in the 79 lambs. Microscopically, Cryptosporidium spp. oocysts were observed in only one lamb on one farm (prevalence 1.3%) and one ewe on another farm (prevalence 1.4%). By contrast, Cryptosporidium spp. DNA was detected in 17 ewes (prevalence ranging from 10.5% to 50% depending on the farm) and in 36 lambs (prevalence ranging from 0% to 77.8% depending on the farm). Only zoonotic Cryptosporidium parvum IId and IIa genotypes were identified when genotyping was possible. Cryptosporidium ubiquitum and C. xiaoi were detected on one and three farms, respectively. We conclude that healthy young lambs and their mothers during the peripartum period could be a source of environmental contamination with oocysts.

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Section: Discussionmentioning

confidence: 89%

Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum

et al. 2020

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“…Previous studies have shown that C. parvum is also dominant in horses from Brazil, the Iberian Peninsula, Italy and New Zealand as well as in other Chinese horses (Table 3). 8–11,13,16,17,19–27 It is thus possible to conclude that zoonotic cryptosporidiosis is caused mostly by C. parvum and, in addition, we can speculate that Cryptosporidium infection in racehorses may be a source of infection for humans and other animals.…”

Section: Discussionmentioning

confidence: 99%

“…Since the first report of cryptosporidiosis in Arabian horses, over 40 epidemiological investigations from 16 countries throughout the world have documented the presence of Cryptosporidium in horses 5,7,16–18 . Seventeen of these studies used molecular biology techniques while most early investigations used Ziehl–Neelsen staining and microscopy of faecal smears 5,8–11,13,16,17,19–28 . Meta‐analysis showed that the pooled prevalence rates were lower with the PCR method (4.7%) than that with the conventional microscopic method (13.8%) 5 .…”

Section: Discussionmentioning

confidence: 99%

Molecular detection and genetic characteristics of Cryptosporidium spp. in Chinese racehorses

Wei

Feng

et al. 2022

Equine Veterinary Journal

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Background: Cryptosporidium is a protozoan parasite causing diarrhoea in humans and animals. Although Cryptosporidium has been found in domestic horses (farmed or kept at pasture), there has been only one published study of Cryptosporidium infections in Chinese racehorses, which was restricted to a very small geographical area.Objectives: To investigate the presence of Cryptosporidium spp. in the faeces of racehorses in China and to perform molecular characterisation of the parasite. Study design: Cross-sectional. Methods: A total of 621 fresh faecal samples were collected for DNA extraction from racehorses at 17 equestrian clubs from 12 provinces of China from December 2016 to May 2018. All the DNA were analysed for the presence of Cryptosporidium species/genotypes and subtypes by PCR amplification of the small subunit ribosomal RNA and 60 kDa glycoprotein genes respectively.Results: PCR analysis revealed that 11 samples (1.8%) were positive for Cryptosporidium spp. Among them seven samples were identified as C. parvum and four were C. hominis.The C. parvum isolates were identified as subtype IIdA14G1 (n = 4) and IIdA15G1(n = 3), while all C. hominis isolates were identified as subtype IkA18G1 (n = 4).Main limitations: A single faecal sample from each horse was used instead of multiple samples that could improve the detection rates of the parasite.Conclusions: Although Cryptosporidium infection rate was relatively low in the investigated racehorses, the presence of zoonotic subtypes IIdA14G and 1IIdA15G1 of C. parvum and IkA18G1 of C. hominis, suggesting that these animals are a potential source of Cryptosporidium in humans.

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“…The IIaA16G1R1 subtype was reported in lamb, calves, and humans, as well as water sources in Romania [ 36 ], Estonia [ 37 ], and Slovakia [ 38 ]. The IIaA16G3R1 subtype was also seen in calves and goats in Spain, England and wild ponies on the Iberian Peninsula [ 39 – 41 ]. A study also reported a high prevalence of C .…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Cryptosporidium spp. from humans in Ethiopia

Woreta

Degarege²,

Adamu³

et al. 2021

PLoS ONE

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Data on the distribution and genotype of Cryptosporidium species is limited in Ethiopia. This study examined the presence and genetic diversity of Cryptosporidium species circulating in Ethiopian human population. Stool samples collected from patients who visited rural (n = 94) and urban (n = 93) health centers in Wurgissa and Hawassa district, respectively, were examined for the presence of Cryptosporidium spp. using microscopy, nested PCR and real-time PCR. To detect infection with PCR, analysis of 18S ribosomal RNA was performed. Subtyping was performed by sequencing a fragment of GP60 gene. The overall prevalence of infection was 46% (n = 86) by microscope and PCR. When 48 (out of 86) PCR positive samples were genotyped, two species were identified: C. parvum (n = 40) and C. hominis (n = 8). When 15 of the 40 C. parvum isolates were subtyped, zoonotic subtypes of IIaA14G1R1 (n = 1), IIaA15G2R1 (n = 1), IIaA16G1R1 (n = 2), IIaA16G3R1 (n = 2), IIaA17G1R1 (n = 1), IIaA19G1R1 (n = 1), IIaA20G1R1 (n = 3), IIaA22G1R1 (n = 1), IIaA22G2R1 (n = 1), IIdA23G1 (n = 1) and IIdA24G1 (n = 1) were identified. When 6 of the 8 C. hominis isolates were subtyped, subtypes IaA20 (n = 5), and IdA21(n = 1) were identified. This study suggests that C. parvum and C. hominis are causes of cryptosporidiosis in human in the Wurgissa district and Hawassa in Ethiopia. Zoonotic transmission might be the main route of transmission.

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First report of zoonotic Cryptosporidium parvum GP60 subtypes IIaA15G2R1 and IIaA16G3R1 in wild ponies from the northern Iberian Peninsula

Cited by 11 publications

References 25 publications

Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum

Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum

Molecular detection and genetic characteristics of Cryptosporidium spp. in Chinese racehorses

Molecular characterization of Cryptosporidium spp. from humans in Ethiopia

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