First report on a variant of <i>Squash leaf curl China virus</i> (SLCCNV) infecting <i>Benincasa hispida</i> in India

Riyaz, S.U. Mohammed; Sundarajan, Deepan; Gunasekaran, Dharanivasan; Jesse, Michael Immanuel; Muthuramalingam, Raja; Kathiravan, K.

doi:10.5197/j.2044-0588.2013.028.020

Cited by 19 publications

(9 citation statements)

References 8 publications

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“…Leaf samples from squash plants displaying symptoms of Begomovirus infection were collected from the field, and the infection status of the samples was confirmed through PCR of Squash Leaf Curl China Virus (SLCCNV), as reported earlier [ 7 ]. The virus particles were then purified using the Cesium sulfate (Cs 2 SO 4 ) density gradient ultracentrifugation (CP100WX, HITACHI, JAPAN) [ 13 ].…”

Section: Methods and Findingsmentioning

confidence: 99%

“…It enables the acquisition of high-resolution virus images without the need for potentially hazardous negative-staining procedures, such as those involving chemically toxic and radioactive substances like Uranyl Acetate [ 6 ]. The study focuses on Begomovirus particles, economically significant plant viruses within the Geminiviridae family, isolated from squash leaf samples [ 7 ]. These viruses possess a non-enveloped structure with single-stranded circular DNA and exhibit twin icosahedral symmetry (T = 1), measuring on average 38 nm in length and 20–28 nm in diameter.…”

Section: Introductionmentioning

confidence: 99%

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Innovative stain-free technique for high-resolution imaging of virus particles via standard transmission electron microscopy

Thangavelu,

Luis da Silva

2024

Heliyon

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Section: Methods and Findingsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Innovative stain-free technique for high-resolution imaging of virus particles via standard transmission electron microscopy

Thangavelu,

Luis da Silva

2024

Heliyon

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“…Begomoviruses possess either monopartite genome DNA A or bipartite genomes DNA A and B (Asigul et al, 2018). Squash leaf curl China virus (SLCCNV) belongs to the genus Begomovirus and is an important bipartite virus that causes severe economic losses in Cucurbitaceae crops such as wax gourd, squash, and pumpkin (Saritha et al, 2011;Riyaz et al, 2013;Maina et al, 2017). The disease incidence in ash gourds can reach 100% in the field (Riyaz et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Squash leaf curl China virus (SLCCNV) belongs to the genus Begomovirus and is an important bipartite virus that causes severe economic losses in Cucurbitaceae crops such as wax gourd, squash, and pumpkin (Saritha et al, 2011;Riyaz et al, 2013;Maina et al, 2017). The disease incidence in ash gourds can reach 100% in the field (Riyaz et al, 2013). SLCCNV infection was first reported in melons in Hainan Province, China (Wu et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

AC5 protein encoded by squash leaf curl China virus is an RNA silencing suppressor and a virulence determinant

Liu

Kang

et al. 2022

Front. Microbiol.

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Squash leaf curl China virus (SLCCNV) is a bipartite Begomovirus. The function of the protein AC5, which is encoded by SLCCNV, is unknown. Here, we confirmed that the 172-amino acids (aa) long AC5 protein of SLCCNV could suppress single-stranded RNA but not double-stranded RNA-induced post-transcriptional gene silencing (PTGS). Furthermore, we determined that the C-terminal domain (96–172 aa) of the AC5 protein was responsible for RNA silencing suppressor (RSS) activity via deletion mutant analysis. The AC5 protein can reverse GFP silencing and inhibit systemic silencing of GFP by interfering with the systemic spread of the GFP silencing signal. The SLCCNV AC5 protein was localized to both the nucleus and cytoplasm of Nicotiana benthamiana cells. Furthermore, deletion analysis showed that the putative nuclear localization signal (NLS, 102–155 aa) was crucial for the RNA silencing suppression activity of AC5. In addition, the AC5 protein elicited a hypersensitive response and enhanced potoao virus X (PVX) RNA accumulation in infected N. benthamiana plants. Using the infectious clones of the SLCCNV and SLCCNV-AC5 null mutants, mutational analysis confirmed that knockout of the AC5 gene abolished SLCCNV-induced leaf curl symptoms, showing SLCCNV AC5 is also a virulence determinant.

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“…Cucurbits are important economic crops, and their growing acreage has reached 7.89 million hectares (ha) with production of more than 238.6 million tons worldwide, with Asian countries being the major contributors [1]. However, leaf curl disease caused by whitefly-transmitted begomoviruses constrains cucurbit production [2][3][4]. Symptoms of cucurbit leaf curl disease include the leaf yellowing, mosaic and curling, and diseased plant may also reveal symptoms of vein swelling, yellow chlorotic spot and plant stunting [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia

Chen

Lai

Lin

et al. 2021

Plants

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Cucurbits are important crops in the world. However, leaf curl disease constrains their production. Here, begomovirus diversity and pathogenicity associated with the disease in Malaysia were studied based on 49 begomovirus-detected out of 69 symptomatic plants from seven cucurbit crops in 15 locations during 2016 and 2017. The presence of Squash leaf curl China virus (SLCCNV) and Tomato leaf curl New Delhi virus (ToLCNDV) were confirmed by virus detection by polymerase chain reaction, viral DNA sequence analysis and specific detection of the viral components. ToLCNDV Malaysian isolates were further distinguished into strains A, B, C and D. Virus co-infection was detected in bitter gourd, bottle gourd and squash. Among them, eight bitter gourd samples were detected without SLCCNV DNA-A. However, one bottle gourd and five squash samples were without ToLCNDV DNA-B. Pseudorecombination of ToLCNDV DNA-A and SLCCNV DNA-B was detected in two bitter gourd samples. The pathogenic viruses and pseudorecombinants were confirmed by agroinoculation. The viral DNA-B influencing on symptomology and host range was also confirmed. The results strengthen the epidemic of cucurbit-infecting begomovirus in Malaysia as well as Southeast Asia. Especially, the natural pseudorecombinant of begomovirus that extends host range and causes severe symptom implies a threat to crops.

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First report on a variant of Squash leaf curl China virus (SLCCNV) infecting Benincasa hispida in India

Cited by 19 publications

References 8 publications

Innovative stain-free technique for high-resolution imaging of virus particles via standard transmission electron microscopy

Innovative stain-free technique for high-resolution imaging of virus particles via standard transmission electron microscopy

AC5 protein encoded by squash leaf curl China virus is an RNA silencing suppressor and a virulence determinant

Genetic Diversity, Pathogenicity and Pseudorecombination of Cucurbit-Infecting Begomoviruses in Malaysia

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