First report on antibody response of Seriola dumerilii (Risso 1810) challenged with Listonella anguillarum

Zaccone, Renata; Mancuso, Monique

doi:10.1016/j.fsi.2008.07.009

Cited by 9 publications

(9 citation statements)

References 20 publications

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“…The ELISA procedure was already reported by Zaccone and Mancuso (2008). 50 μl of a monoclonal antibody Anti-European sea bass IgM (Aquatic diagnostics) diluted with TTBS + 3% of skimmed milk was used as specific antibody and incubated for 30 min at 37 °C.…”

Section: Indirect Elisa Protocolmentioning

confidence: 99%

“…The production of antibodies, as a consequence of natural or experimental outbreaks of disease, was studied in several fish species such as the rainbow-trout Inside of a research project "Monitoring of pasteurellosis and vibriosis in fish species of new interest for aquaculture: testing rapid diagnostic methods and possible correlations with challenges in microcosm", studies on humoral immune response of teleosts were carried out using a new indirect ELISA protocol (Zaccone, Mancuso, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Detection of Specific Antibody Levels against Photobacterium damselae subsp. piscicida in Sea Bass during Experimental Challenge

Mancuso¹

2016

JMBA

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The detection of specific antibodies in fish serum is an important assay for disease diagnosis and may furnish information on the real status of fish immunization to infectious disease. To evaluate the immune response of sea bass (Dicentrarchus labrax) against photo bacterium damselae subsp. piscicida, during an experimental challenge specific antibodies were on serum samples detected by an indirect ELISA protocol. Results showed the presence of antibodies against-P. d. p. in fish sera ten days after challenge, indicating that the specimens began to react to the pathogen.

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Section: Indirect Elisa Protocolmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of Specific Antibody Levels against Photobacterium damselae subsp. piscicida in Sea Bass during Experimental Challenge

Mancuso¹

2016

JMBA

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“…Blood samples were incubated for 24 h at 25°C in marine broth (Microbial Diagnostic) and incubated for the same time at the same temperature in marine agar (Microbial Diagnostics). The growth colonies were isolated in pure culture and then the bacteriological assays were performed to identify the pathogen using the protocol of Zaccone & Mancuso (2008).…”

Section: Bacteriological Assaymentioning

confidence: 99%

Induction of mild enterocolitis in zebrafish Danio rerio via ingestion of Vibrio anguillarum serovar O1

Randazzo¹,

Abbate²,

Marino³

et al. 2015

Dis. Aquat. Org.

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Vibrio anguillarum is the etiological agent of a fatal hemorrhagic disease known as vibriosis that affects a wide range of fish species, causing severe economic losses. Several investigations have been carried out to elucidate the virulence mechanisms of this pathogen and to develop rapid detection techniques and effective disease-prevention strategies. The aim of our study was to evaluate the most effective way to induce mild enteritis in a fish model, in order to allow further applications. The experiments were carried out using 2 methods of administration of V. anguillarum serotype O1 to adult zebrafish Danio rerio: via intraperitoneal injection and via ingestion of infected Artemia nauplii. The results showed that the intraperitoneal administration often caused massive fish death due to severe systemic involvement. In our experiments, the effect of intraperitoneal infection was evident 48 h post infection, with cumulative mortality within 7 d post infection with severe histopathological changes in kidney hematopoietic tissue and in the intestine. In contrast, oral infection via Artemia did not show systemic involvement and only a moderate degree of inflammatory influx of the mucosa, a partial recovery at 12 d post infection, and no mortality. For these reasons, oral infection with live food appears to be the most effective method to induce mild enteritis with a local inflammatory response.

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“…Moreover its important use serological methods that measure antibody responses in serum samples and it's a not-lethal method [19].…”

Section: Hard Evidence For Transmission Between Wild and Farmed Fish mentioning

confidence: 99%

Photobacteriosis Exchange between Wild and Farmed Fish in the Mediterranean Area

Mancuso¹

2012

J Aquacult Res Dev

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Photobacteriosis appeared for the first time in the Mediterranean area in 1990 causing heavy losses in fish-farms and affecting also the open sea. It affects several fish species, the aetiological agent is Photobacterium damselae subsp. piscicida. The disease is influenced by climatic changes and depends from the age of fish. The bacterium may survive in water and sediment for one month and can entry in a viable but nonculturable (VBNC) state and moreover may be transmitted to fish through water using the skin as a portal to entry. The potential exchange of pathogens between farmed and wild fish is already object of debate. Great efforts are necessary to better understand the dynamics of the disease and monitoring plans and facilities management.

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First report on antibody response of Seriola dumerilii (Risso 1810) challenged with Listonella anguillarum

Cited by 9 publications

References 20 publications

Detection of Specific Antibody Levels against Photobacterium damselae subsp. piscicida in Sea Bass during Experimental Challenge

Detection of Specific Antibody Levels against Photobacterium damselae subsp. piscicida in Sea Bass during Experimental Challenge

Induction of mild enterocolitis in zebrafish Danio rerio via ingestion of Vibrio anguillarum serovar O1

Photobacteriosis Exchange between Wild and Farmed Fish in the Mediterranean Area

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