First report on in vivo fertility trial of frozen thawed boar semen in India#

Abstract

The present study was conducted to evaluate the in vivo fertility of frozen thawed boar semen. Twenty ejaculates collected from six mature boars were frozen in a programmable freezer. After freezing the semen was evaluated for different sperm quality parameters. Twenty five sows were inseminated artificially utilizing frozen thawed semen. The percentage of sperm motility, live sperm, live intact acrosome, plasma membrane intact sperm, HOST-reacted sperm, live sperm with high mitochondrial membrane potential, lipid peroxidised sperm and DNA-damaged sperm of frozen semen utilised for AI were 56.25 ± 0.96, 63.75 ± 1.47, 59.88 ± 1.09, 41.08 ± 1.01, 40.31 ± 1.02, 86.23 ± 1.29, 9.28 ± 0.83 and 4.20 ± 0.29 respectively. The farrowing rate was 44.00 per cent and the mean litter size at birth was 5.91 ± 0.69. It could be concluded that the freezing and insemination protocol of boar semen used in the present study resulted in moderate fertility of frozen boar semen and it would help in further improvement and utilization of frozen boar semen for AI in India.

Semen quality and fertility of liquid stored and frozen-thawed semen in crossbred pigs of North-Eastern India

“…There was reduction in sperm motility and viability by 20-25% after freezing. In agreement with our results, similar range of postthaw sperm motility and viability were recorded recently (Baishya et al, 2015). Liquid semen maintained significantly (p<0.01) higher sperm motility, viability and plasma membrane integrity from day 0 to 3 as compared to frozenthawed semen (Table 1).…”

“…Recently, Kadirvel et al (2016) also reported that the farrowing rate was significantly superior for semen that used from initial days of preservation as compared to aged semen. The pregnancy rate and farrowing rate recorded in the present study for frozen-semen is in close agreement with Baishya et al (2015) recorded recently, but higher than that reported by Apic et al (2015) and lower than that reported by Didion et al (2011). The study recorded significantly (p<0.01) lower pregnancy rate, farrowing rate and litter size in frozen thawed semen as compared to liquid semen (Table 2).…”

​Study of the Relationship between the Levels of L-carnitine and NO Metabolites in Blood Plasma and Seminal Plasma in Stallions

“…Artificial insemination with chilled sperm is a reproductive biotechnology often used in horse breeding (Olaciregui et al, 2014). However, maintaining the acceptable sperm quality after cooling, which affects the frequency of pregnancy, remains an urgent problem at the present time (Baishya et al, 2015;Pathak et al, 2020;Ugur et al, 2019). The exact reasons for the decreased sperm motility after cryopreservation remain unclear.…”