First report on molecular basis of potato leaf roll virus (PLRV) aggravation by combined effect of tuber and prevailing aphid

Kumar, Ravi Ranjan; Ansar, Mohammad; Rajani, Kumari; Kumar, Jitesh; Ranjan, Tushar

doi:10.21203/rs.3.rs-42627/v4

Cited by 1 publication

(4 citation statements)

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“…To the best of our knowledge, this is the first study that presents a novel P-loop containing ATPase fold of CP comprising several repeat motifs and demonstrates that the suppression of CP expression using siRNA constructs can lead to resistance against PLRV. The inhibition of the expression of CP by gene silencing is an efficient and promising method to introduce resistance to PLRV [2, 4–5, 21]. As PLRV causes severe crop yield losses in the potato growing regions worldwide [22–23], our findings will be helpful for developing PLRV-resistant potato crops.…”

Section: Discussionmentioning

confidence: 94%

“…Among these stresses, viruses have a significant contribution to yield losses worldwide [1]. Due to vegetative reproduction of potato, viruses propagate through seed tubers and maintain their life cycle [2]. Tubers used for planting in the next season can harbor latent viruses, which subsequently reduce emergence, plant vigor, and yield [2].…”

Section: Introductionmentioning

confidence: 99%

“…Due to vegetative reproduction of potato, viruses propagate through seed tubers and maintain their life cycle [2]. Tubers used for planting in the next season can harbor latent viruses, which subsequently reduce emergence, plant vigor, and yield [2]. More than 40 different viruses affect the cultivation of potato crop across the globe [1–2].…”

Section: Introductionmentioning

confidence: 99%

“…Tubers used for planting in the next season can harbor latent viruses, which subsequently reduce emergence, plant vigor, and yield [2]. More than 40 different viruses affect the cultivation of potato crop across the globe [1–2]. Potato leaf roll virus (PLRV), which belongs to the genus Polerovirus and family Luteoviridae , is a ubiquitous potato virus worldwide and is responsible for more than 20 million tons yield loss every year [3–4].…”

Section: Introductionmentioning

confidence: 99%

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Knockdown of a novel ATPase domain of capsid protein inhibits genome packaging in potato leaf roll virus

Kumar

Das

et al. 2021

Preprint

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Potato leaf roll virus (PLRV) uses powerful molecular machines to package its genome into a viral capsid employing ATP as fuel. Although, recent bioinformatics and structural studies have revealed detailed mechanism of DNA packaging, little is known about the mechanochemistry of genome packaging in small plant viruses such as PLRV. We have identified a novel P-loop-containing ATPase domain with two Walker A-like motifs, two arginine fingers, and two sensor motifs distributed throughout the polypeptide chain of PLRV capsid protein (CP). The composition and arrangement of the ATP binding and hydrolysis domain of PLRV CP is unique and rarely reported. The discovery of the system sheds new light on the mechanism of viral genome packaging, regulation of viral assembly process, and evolution of plant viruses. Here, we used the RNAi approach to suppress CP gene expression, which in turn prevented PLRV genome packaging and assembly in Solanum tuberosum cv. Khufri Ashoka. Potato plants agroinfiltrated with siRNA constructs against the ATPase domain of CP exhibited no rolling symptoms upon PLRV infection, indicating that the silencing of CP gene expression is an efficient method for generating PLRV-resistant potato plants. Moreover, our findings provide a robust approach to generate PLRV-resistant potato plants, which can be further extended to other species. Finally, we propose a new mechanism of genome packaging and assembly in plant viruses.

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Section: Discussionmentioning

confidence: 94%