First reported detection of the mobile colistin resistance genes, mcr-8 and mcr-9, in the Irish environment

Cahill, Niamh; Hooban, Brigid; Fitzhenry, Kelly; Joyce, Aoife; O’Connor, Louise; Miliotis, Georgios; McDonagh, Francesca; Burke, Liam; Chueiri, Alexandra; Farrell, Maeve Louise; Bray, James E.; DeLappe, Niall; Brennan, Wendy; Prendergast, Deirdre M.; Gutiérrez, Montserrat; Burgess, Catherine M.; Cormican, Martin; Morris, Dearbháile

doi:10.1016/j.scitotenv.2023.162649

Cited by 4 publications

(4 citation statements)

References 71 publications

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“…Distinct seasonal mcr profiles were observed in air and liquid samples at each WWTP, with the most frequently identified gene variant being mcr -5, followed by mcr -8. The prevalence of mcr -5 across wastewater samples supports previous findings that observed high levels of this variant in hospital and municipal wastewater. ,− Interestingly, while mcr -8 was the second most abundant variant in this study, it has not previously been identified in municipal wastewater and is most often associated with aquatic environments. ,, …”

Section: Resultssupporting

confidence: 89%

“…It is possible that the increased diversity of mcr variants observed in the low biomass air samples as compared to the high biomass liquid sludge samples may be due to differences in metagenomic coverage, and more gene diversity may be identified in the liquid samples with more extensive sequencing. However, even with this limitation, the results of this study support previous findings that observed a range of mcr variants in hospital and municipal wastewater. ,− ,, Additionally, this study is one of the first to examine seasonal patterns of mcr abundance and expands the understanding of environmental routes of mcr dissemination with the identification of mcr variants in WWTP bioaerosols.…”

Section: Resultssupporting

confidence: 83%

“…While K. pneumoniae is the most commonly identified mcr -8 host, , it has also been identified in a Stenotrophomonas strain isolated from poultry farm sewage …”

Section: Resultsmentioning

confidence: 99%

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Seasonal Patterns in Mobile Colistin Resistance Gene Variants in Wastewater Bioaerosols and Liquid Sludge

Isanovic,

Correa Velez,

Viner

et al. 2024

ACS EST Water

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To address the growing threat of global antimicrobial resistance, a one-health approach is needed to understand the complex socioecological cycling of antibiotic resistance genes. In this study, a metagenomics approach using DNA shotgun sequencing, metagenome assembly, and antibiotic resistance gene (ARG) annotation was used to examine seasonal patterns in the abundance of mobile colistin resistance (mcr) gene variants in bioaerosols and liquid sludge in three wastewater treatment plants (WWTPs). ARGs represented 0.2−0.8 and 0.1−0.2% of the bioaerosol and liquid sludge metagenomes, respectively, while mcr genes represented 0−0.3 and 0−0.5% of the identified ARGs in bioaerosol and liquid sludge metagenomes. Seven of the ten known mcr variants were detected in wastewater bioaerosol and liquid samples, with mcr-5 and mcr-8 being the most prevalent across all seasons and sites. Additional functional and taxonomic annotation of mcr-containing metagenomic contigs showed that mcr genes were often located on contigs with other co-occurring ARGs and mobile genetic elements and may be harbored by opportunistic human pathogens and other bacterial taxa not previously associated with mcr genes. Atmospheric dispersion modeling showed that mcr-containing bioaerosols can be transported kilometers away from the WWTPs, resulting in the possible dissemination of these ARGs into surrounding environments and communities.

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Section: Resultssupporting

confidence: 89%

Section: Resultssupporting

confidence: 83%

See 1 more Smart Citation

Seasonal Patterns in Mobile Colistin Resistance Gene Variants in Wastewater Bioaerosols and Liquid Sludge

Isanovic,

Correa Velez,

Viner

et al. 2024

ACS EST Water

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“…In addition, Escherichia coli co-harboring conjugative plasmids with colistin-and carbapenem resistance genes have a high prevalence and have been identified from different functional areas of an urban wastewater treatment plant in China [2]. Moreover, the mobile colistin resistance (mcr) genes mcr-8 and mcr-9 were the first reported in Ireland, with the mcr-8-positive Klebsiella pneumoniae displaying resistance to colistin [3]. The global spread of the mcr gene highlights that the addition of colistin to veterinary drugs affects human health.…”

Section: Introductionmentioning

confidence: 99%

The Molecular Identification and Comprehensive Analysis of Klebsiella pneumoniae Isolated from Industrial Wastewater

Yan,

Li,

Wang

et al. 2024

Separations

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Industrial wastewater typically contains many organic and inorganic pollutants and is also contaminated by various microorganisms. Microbial species in industrial wastewater have not been extensively investigated. In this experiment, a Klebsiella pneumoniae strain was isolated for the first time from industrial wastewater containing a high concentration of sulfate and phosphate. Mass spectrometry, genetic analysis, and biochemical identification were conducted to understand the genetic and biochemical characteristics of this Klebsiella pneumoniae strain recovered from industrial wastewater. Growth experiments revealed that it exhibited an excellent growth rate in nutrient broth. Further analyses showed that the strain was sensitive to most antibiotics but resistant to chloramphenicol and nitrofurantoin. It also exhibited significant resistance to piperacillin/tazobactam and cefotaxime/clavulanic acid. Resistance gene experiments indicated the presence of gyrA, OqxB, and ParC genes associated with antibiotic resistance in the isolated Klebsiella pneumoniae strain. Proteomics uncovered the following three proteins related to drug resistance: the multi-drug resistant outer membrane protein MdtQ, the multi-drug resistant secretion protein, and the modulator of drug activity B, which are coexistent in Klebsiella pneumoniae. Proteomics and bioinformatics analyses further analyzed the protein composition and functional enrichment of Klebsiella pneumoniae. The isolation of Klebsiella pneumoniae from a high concentration in sulfate and phosphate industrial wastewater provides a new direction for further research on the characteristics and drug resistance traits of industrial wastewater microorganisms and the potential risks they may pose when released into the environment.

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Transcriptomic analysis of induced resistance to polymyxin in carbapenem-resistant Enterobacter cloacae complex isolate carrying mcr-9

Wu,

Liu,

Wang

et al. 2024

Journal of Global Antimicrobial Resistance

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First reported detection of the mobile colistin resistance genes, mcr-8 and mcr-9, in the Irish environment

Cited by 4 publications

References 71 publications

Seasonal Patterns in Mobile Colistin Resistance Gene Variants in Wastewater Bioaerosols and Liquid Sludge

Seasonal Patterns in Mobile Colistin Resistance Gene Variants in Wastewater Bioaerosols and Liquid Sludge

The Molecular Identification and Comprehensive Analysis of Klebsiella pneumoniae Isolated from Industrial Wastewater

Transcriptomic analysis of induced resistance to polymyxin in carbapenem-resistant Enterobacter cloacae complex isolate carrying mcr-9

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