First reported incidence of Bacillus velezensis exhibiting effective antagonism against a blowfly species, Lucilia cuprina

Ramesar, Danvir Rajesh; Hunter, Charles

doi:10.1007/s10526-022-10172-4

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…Further, when different families are co-produced, they can exhibit synergistic interactions and enhanced activities [ 19 ]. Lipopeptides produced by Bacillus exhibit specific insecticidal activities that can affect various insect orders including Hemiptera [ 20 ], Lepidoptera [ 21 , 22 ], Diptera [ 23 , 24 ]. Thus, Bacillus may represent a promosing microbial resource with insecticidal potential.…”

Section: Introductionmentioning

confidence: 99%

Lipopeptides from Bacillus velezensis ZLP-101 and their mode of action against bean aphids Acyrthosiphon pisum Harris

Liu,

Zhao,

et al. 2024

BMC Microbiol

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Background Natural products are important sources for the discovery of new biopesticides to control the worldwide destructive pests Acyrthosiphon pisum Harris. Here, insecticidal substances were discovered and characterized from the secondary metabolites of the bio-control microorganism Bacillus velezensis strain ZLP-101, as informed by whole-genome sequencing and analysis. Results The genome was annotated, revealing the presence of four potentially novel gene clusters and eight known secondary metabolite synthetic gene clusters. Crude extracts, prepared through ammonium sulfate precipitation, were used to evaluate the effects of strain ZLP-101 on Acyrthosiphon pisum Harris aphid pests via exposure experiments. The half lethal concentration (LC50) of the crude extract from strain ZLP-101 against aphids was 411.535 mg/L. Preliminary exploration of the insecticidal mechanism revealed that the crude extract affected aphids to a greater extent through gastric poisoning than through contact. Further, the extracts affected enzymatic activities, causing holes to form in internal organs along with deformation, such that normal physiological activities could not be maintained, eventually leading to death. Isolation and purification of extracellular secondary metabolites were conducted in combination with mass spectrometry analysis to further identify the insecticidal components of the crude extracts. A total of 15 insecticidal active compounds were identified including iturins, fengycins, surfactins, and spergualins. Further insecticidal experimentation revealed that surfactin, iturin, and fengycin all exhibited certain aphidicidal activities, and the three exerted synergistic lethal effects. Conclusions This study improved the available genomic resources for B. velezensis and serves as a foundation for comprehensive studies of the insecticidal mechanism by Bacillus velezensis ZLP-101 in addition to the active components within biological control strains.

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Section: Introductionmentioning

confidence: 99%

Lipopeptides from Bacillus velezensis ZLP-101 and their mode of action against bean aphids Acyrthosiphon pisum Harris

Liu,

Zhao,

et al. 2024

BMC Microbiol

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Manure trial evaluation of Bacillus velezensis PHP1601 as a viable biocontrol candidate against nuisance fly species at an operational piggery

Ramesar,

Hunter

2023

BioControl

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Fly infestations threaten farming and livestock operations, contributing to animal irritation and the spread of serious diseases. In this study, the application of Bacillus velezensis PHP1601 (Bacillales: Bacillaceae) treatments (105 and 1010 endospore g−1) to pig manure slurries was evaluated as a means of controlling fly reproductive cycles. Two cycles of fly emergence were evaluated over a 33 days period for each replicated trial. For both treatments, the first emergence event resulted in a significant reduction in the percentage of flies emerging compared to an un-treated control. Extended monitoring of the biocontrol containers revealed that fly emergence was completely eliminated by the time that a second round of fly emergence was observed for the control. A B. velezensis-specific real-time PCR method was developed and used to assess the population dynamics of the applied biocontrol agent over the course of the experiment. Strain PHP1601 remained viable in the manure and cell numbers increased by several orders of magnitude. REP-PCR fingerprinting was used to confirm the clonality of endospores recovered from the manure. Fly species recovered from the trials were identified by cytochrome oxidase gene barcode sequencing. Several species of veterinary and medically significant flies were identified. They were all deemed to be susceptible to treatments with PHP1601 and constituted part of the strain's host range. The study demonstrated the effectiveness of B. velezensis PHP1601 as a promising biocontrol agent for controlling fly infestations under conditions similar to its intended use.

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Elucidation of lipopeptide biosurfactants responsible for the larvicidal activity of Bacillus velezensis PHP1601 towards Lucilia cuprina larvae

Ramesar

Hunter

2023

Biocontrol Science and Technology

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First reported incidence of Bacillus velezensis exhibiting effective antagonism against a blowfly species, Lucilia cuprina

Cited by 3 publications

References 36 publications

Lipopeptides from Bacillus velezensis ZLP-101 and their mode of action against bean aphids Acyrthosiphon pisum Harris

Lipopeptides from Bacillus velezensis ZLP-101 and their mode of action against bean aphids Acyrthosiphon pisum Harris

Manure trial evaluation of Bacillus velezensis PHP1601 as a viable biocontrol candidate against nuisance fly species at an operational piggery

Elucidation of lipopeptide biosurfactants responsible for the larvicidal activity of Bacillus velezensis PHP1601 towards Lucilia cuprina larvae

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