First Seroprevalence Study of Coxiella burnetii in Rural Pregnant Women in Contact with Livestock in Khorramabad

Ghobadi, Eiman Azizyari; Jaydari, Amin; Akbari, Soheila; Anbari, Khaterh

doi:10.5812/iji.97850

Cited by 3 publications

(1 citation statement)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Vaccines are available for animals. Q fever vaccination is recommended for people in high risk professions, but can cause severe side effects [39][40][41].…”

Section: Discussionmentioning

confidence: 99%

Serological Prevalence of and Risk Factors for Coxiella burnetti Infection in Women of Punjab Province, Pakistan

Ali

Saeed

Rizwan

et al. 2022

IJERPH

View full text Add to dashboard Cite

Background: Coxiella burnetii, the etiological agent of Q (query) fever, provokes abortions in ruminants and is suspected to cause adverse pregnancy outcomes in women. Infection of pregnant women is linked with high mortality and morbidity of the fetus and the mother is at high risk to acquire chronic Q fever. This research was conducted to evaluate the prevalence of Q fever in women and to detect associated risk factors in four districts of Punjab Province, Pakistan. Methods: A total of 297 blood samples were obtained from 147 pregnant and 150 non-pregnant women of the districts Okara, Jhang, Chiniot and Faisalabad of Punjab, Pakistan. Data related to risk factors and demographic parameters were collected using a questionnaire. Serum samples were screened for phase I and phase II specific IgG antibodies for antigens of phase I and phase II using ELISA tests. Univariate and binary regression were used to analyze important risk factors of Q fever. Results: Twenty-five serum samples (8.4%) were found seropositive for Q fever. Seventeen women were positive for Phase-I and twenty-one were positive for phase-II antibodies. Highest and statistically significant (p < 0.05) seroprevalence of 17.1% was observed in Faisalabad. Age, urbanicity, living status, pregnancy status, abortion history, occupation, and consumption of tap water were positively correlated (p < 0.05) with Q fever, while being aged, urbanity, low income, contact with animals and consumption of tap water was identified as potential risk factors. Conclusions: Q fever is prevalent in women of Pakistan. There is a need for an awareness program about the importance of C. burnetii infections and prevention strategies in women during pregnancy to minimize adverse pregnancy outcomes.

show abstract

“…Vaccines are available for animals. Q fever vaccination is recommended for people in high risk professions, but can cause severe side effects [39][40][41].…”

Section: Discussionmentioning

confidence: 99%

Serological Prevalence of and Risk Factors for Coxiella burnetti Infection in Women of Punjab Province, Pakistan

Ali

Saeed

Rizwan

et al. 2022

IJERPH

View full text Add to dashboard Cite

show abstract

Molecular examination for Coxiella burnetii and Brucella spp. infections in Iranian women experiencing spontaneous miscarriage

Baseri,

Omidi,

Latifian

et al. 2024

BMC Infect Dis

View full text Add to dashboard Cite

Background Spontaneous miscarriage, a leading health concern globally, often occurs due to various factors, including infections. Among these, Coxiella burnetii and Brucella spp. may have adverse effects on pregnancy outcomes. While previous research has established a link between infections and spontaneous miscarriage, our study aimed specifically to investigate the presence of these two pathogens in abortion samples from women who experienced spontaneous miscarriages in Iran. Our study can add to the existing knowledge by focusing on Iran, a region with a high prevalence of C. burnetii and Brucella spp. As a result, it could provide a better understanding and unique insights into the relationship of these pathogens with spontaneous miscarriages in endemic regions. Methods From March 2021 to March 2022, a total of 728 abortion samples (including placenta and cotyledon) were collected from 409 women who had experienced spontaneous miscarriages in the provinces of Tehran, Fars, and West Azerbaijan in Iran. The specimens included 467 Formalin-Fixed Paraffin-Embedded (FFPE) and 261 fresh frozen samples. After DNA extraction from abortion samples, the quantitative real-time PCR (qPCR) assay targeted a specific fragment of the IS1111 and IS711 elements for molecular identification of C. burnetii and Brucella spp., respectively. Furthermore, the qPCR assay employing specific primers for different species was used to determine the species of Brucella. Results Among the studied women, 1 out of 409 (0.24%) samples tested positive for Brucella spp., specifically Brucella melitensis. There were no positive specimens for C. burnetii. Conclusions Our study contributes to understanding the potential involvement of Brucella species in spontaneous infectious abortion within endemic regions. The identification of B. melitensis in this study highlights the need for further research in this area. However, while our results suggest a relatively low or zero identification of these pathogens in our sample population, this does not rule out the possibility of undetected infections. Therefore, it is critical to acknowledge the limitations of the molecular techniques used (qPCR), which may have potential limitations such as sensitivity and specificity. Moreover, because 64.15% of our samples were FFPE, the sensitivity of the qPCR test may be reduced. These raise concerns about the accuracy of the reported prevalence rates and the potential for false positives or negatives.

show abstract

Seroprevalence of Q Fever in Lorestan Province Veterinary Staff Using IFA Method

Soleimani

Jaydari

2021

Int J Infect

Self Cite

View full text Add to dashboard Cite

Background: Q fever is caused by the bacterium Coxiella burnetii. To date, no studies have been conducted on the rate of Q fever infection among the veterinary staff in Lorestan province. Objectives: The present study aimed to investigate the seroprevalence of infection in the veterinary staff. Methods: This cross-sectional study was conducted from April to July 2019 on 92 samples collected randomly from Lorestan province on veterinary staff who had a history of contact with livestock and were tested via indirect immunofluorescence kit. Results: In this study, 77 (83/69%) samples were positive, and 15 (16/3%) samples were negative. There were no statistically significant relationships between residence, contact with livestock, and work experience. Conclusions: According to the results of this study, the high seroprevalence of a Q fever was observed among Veterinary staff, which further reveals the necessitates of diagnosis, treatment, and prevention of the disease in order to prevent unwanted complications in people in contact with livestock.

show abstract

First Seroprevalence Study of Coxiella burnetii in Rural Pregnant Women in Contact with Livestock in Khorramabad

Cited by 3 publications

References 20 publications

Serological Prevalence of and Risk Factors for Coxiella burnetti Infection in Women of Punjab Province, Pakistan

Serological Prevalence of and Risk Factors for Coxiella burnetti Infection in Women of Punjab Province, Pakistan

Molecular examination for Coxiella burnetii and Brucella spp. infections in Iranian women experiencing spontaneous miscarriage

Seroprevalence of Q Fever in Lorestan Province Veterinary Staff Using IFA Method

Contact Info

Product

Resources

About