First Shiga Toxin-Producing<i>Escherichia coli</i>Isolate from a Patient with Hemolytic Uremic Syndrome, Brazil

Guth, Beatriz E. C.; Souza, Renato Lopes de; Vaz, Tânia; Irino, Kinue

doi:10.3201/eid0805.010419

Cited by 55 publications

(51 citation statements)

References 8 publications

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“…Although a few EHEC/STEC strains were isolated in the 80s (Giraldi et al 1990, Gomes et al 1996, they seem to be occurring more frequently in the last few years. In addition to the strains detected in this study, three O157:H7 strains and one O26:H11 strain (strain isolated from a case of HUS - Guth et al 2002) have been reported in São Paulo. Cantarelli et al (2000) also reported one instance of O91:H21 in Porto Alegre.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil

Franzolin

Alves

Keller

et al. 2005

Mem. Inst. Oswaldo Cruz

107

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Section: Discussionmentioning

confidence: 99%

Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil

Franzolin

Alves

Keller

et al. 2005

Mem. Inst. Oswaldo Cruz

107

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“…In fact, in some geographic areas STEC strains other than O157:H7 are more commonly isolated from humans, and increasing numbers of reports have documented its occurrence in association with sporadic cases of diarrhea and HUS in many countries (4,5,7,8,36). In Brazil, non-O157:H7 STEC strains have also been more frequently isolated from human infections; and serotypes O26:H11, O103:H2, and O111:H8(H Ϫ ) (where H Ϫ is nonmotile) accounted for most of the cases (19,20,42; K. Irino et al, unpublished data).…”

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confidence: 99%

Genetic Heterogeneity of Shiga Toxin-Producing Escherichia coli Strains Isolated in São Paulo, Brazil, from 1976 through 2003, as Revealed by Pulsed-Field Gel Electrophoresis

et al. 2006

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The pulsed-field gel electrophoresis (PFGE) patterns of 46 Shiga toxin-producing Escherichia coli (STEC) strains isolated in São Paulo, Brazil, during the period from 1976 to 2003 were compared with those found among 30 non-STEC strains that carried eae and that belonged to the same serogroups as the STEC strains. All except two of the STEC and non-STEC strains of human origin were from sporadic and unrelated cases of infection; two O111 strains originated from the same patient. Multiple PFGE patterns were found among STEC strains of distinct serotypes. Moreover, the PFGE restriction patterns of STEC strains differed substantially from those observed among non-STEC strains of the same serogroup except serotype O26 strains. Based on the indistinguishable PFGE pattern for two O157:H7 STEC strains isolated in the same geographic area at an interval of approximately 15 days and toxin profile data, the first occurrence of an O157:H7 outbreak in Brazil during that period can be suggested. In general, a close relationship between types of intimin, serotypes, and diarrheagenic groups of E. coli was observed. This is the first time that a large collection of STEC strains from Brazil has been analyzed, and a great genetic diversity was shown among O157:H7 and non-O157:H7 STEC strains isolated in São Paulo, Brazil.

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“…As enteroemolisinas são outro tipo de hemolisinas relacionadas recentemente às linhagens verotoxigênicas de E. coli, isoladas de pacientes humanos com distúrbios entéricos e renais, incluindo no Brasil (Guth et al, 2002). Em animais, as enteroemolisinas estão associadas às linhagens de E. coli isoladas de bovinos com e sem diarréia.…”

Section: Discussão E Conclusõesunclassified

Fatores de virulência em linhagens de Escherichia coli isoladas de mastite bovina

Ribeiro

Costa²,

Leite

et al. 2006

Arq. Bras. Med. Vet. Zootec.

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RESUMOAvaliou-se a ocorrência de fatores de virulência e do sorotipo O157:H7 em 120 linhagens de Escherichia coli, isoladas de 80 casos de mastite clínica bovina e 40 de mastite subclínica. Verificou-se alfahemolisina em oito (6,7%) linhagens, isoladas de cinco casos de mastite clínica e três de mastite subclínica e em nenhuma das estirpes detectou-se enteroemolisina. A presença de sideróforos foi encontrada em 11 (9,2%) linhagens, sete de mastite clínica e quatro de subclínica. Em duas (1,7%) estirpes isoladas de mastite subclínica, identificou-se enterotoxina STa. Observou-se efeito citopático em células vero compatível com a produção de verotoxina-VT em cinco (4,2%) linhagens, duas de mastite clínica e três subclínicas. Em uma (0,8%) linhagem isolada de mastite clínica, detectou-se efeito citopático compatível com o fator necrosante citotóxico. Nenhuma estirpe apresentou-se sorbitol-negativa no MacConkey-sorbitol, tampouco aglutinou com o sorotipo O157:H7. Os antimicrobianos mais efetivos foram polimixina B (97,5%) e norfloxacina (95,8%). Observou-se multi-resistência a dois ou mais antimicrobianos em 24 (20%) estirpes, principalmente com o uso de ampicilina e ceftiofur.Palavras-chave: bovino, mastite, Escherichia coli, virulência, toxina, sorotipo O157:H7 ABSTRACT The occurrence of different virulence factors and O157:H7 serotype investigation in 120 Escherichia coli strains isolated from clinical (80 cases) and subclinical (40 cases) bovine mastitis was evaluated. Alphahaemolysin was detected in 8 (6.7%) strains (5 clinical and 3 subclinical cases). None strain showed enterohaemolysin production. E. coli growth under iron restriction conditions (siderophores production) was observed in 11 (9.2%) strains (7 clinical and 4 subclinical cases). STa enterotoxin was detected in 2 (1.7%) strains from subclinical cases. Cytotoxic effect in vero cells compatible with verotoxin-VT production was observed in 5 (4.2%) strains (2 clinical and 3 subclinical cases

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First Shiga Toxin-ProducingEscherichia coliIsolate from a Patient with Hemolytic Uremic Syndrome, Brazil

Cited by 55 publications

References 8 publications

Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil

Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil

Genetic Heterogeneity of Shiga Toxin-Producing Escherichia coli Strains Isolated in São Paulo, Brazil, from 1976 through 2003, as Revealed by Pulsed-Field Gel Electrophoresis

Fatores de virulência em linhagens de Escherichia coli isoladas de mastite bovina

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