First-time report of metalloproteinases in fish bile and their potential as bioindicators regarding environmental contamination

Study of the bile proteome of Atlantic cod ( Gadus morhua ): Multi-biological markers of exposure to polycyclic aromatic hydrocarbons

“…Despite the growing interest for proteomics in fish (e.g. Bohne-Kjersem et al, 2009;Karim et al, 2011), few studies regarding proteins in the fish bile have been reported so far (Hauser-Davis et al, 2012a, 2012b. The work described herein is the first report regarding the bile proteome of Atlantic cod (Gadus morhua).…”

“…Biliary excretion offered a way to analyze the effect of contaminants and its use as an indicator of exposure for environmental monitoring is confirmed using a proteomic approach (Hauser-Davis et al, 2012a;2012b).…”

“…In addition to salts, bile acid, pigments and metabolites, fish bile also contains a range of peptides and proteins (Hauser-Davis et al, 2012a, 2012b.…”

“…Fish bile is a particularly difficult matrix to work with, and simple but effective clean up steps (to avoid protein loss) are required in order to secure sufficient sample quality for proteomics analyses (Hauser-Davis et al, 2012a). In addition to salts, bile acid, pigments and metabolites, fish bile also contains a range of peptides and proteins (Hauser-Davis et al, 2012a, 2012b.…”

Biological Responses of in vivo Studies to Contaminants: A Contribution to Improve Public Health Knowledge

“…Adverse changes on liver of Poecilia vivipara assessed semi-quantitatively using the (Histopathological Alterations Index -HAI) were described and the possibility of relationship to pesticides, heavy metals, sewage and others factors were postulated [53]. Other potential markers on environmental pollution were illustrated by analyse of matrix metalloproteinases on fish bile from Mugil liza and Tilapia rendalli [54].…”