First Transplantation of Isolated Murine Follicles in Alginate

Vanacker, Julie; Dolmans, Marie-Madeleine; Luyckx, Valérie A.; Donnez, Jacques; Amorim, Christiani Andrade

doi:10.2217/rme.14.33

Cited by 62 publications

(47 citation statements)

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“…A number of variant polymers, classified as natural and synthetic polymers, have been reported for the construction of artificial ovaries [2]. Here, alginate acid sodium salt, as a type of hydrogel, was selected for the modeling of the proposed artificial oocyte due to its versatility, and also the fact that it has been reported as a suitable material for transplantable artificial ovary matrix [15], [16].…”

Section: Model Presentationmentioning

confidence: 99%

“…It is biocompatible and shows non antigenicity [4]. Alginate was also proposed as a candidate material for transplantable artificial ovary matrices in 2012 [3], [5]. It has also been reported that different combinations with alginate such as matrigel could be successfully employed for transplanting murine ovarian cells [3].…”

Section: Introductionmentioning

confidence: 99%

“…Alginate was also proposed as a candidate material for transplantable artificial ovary matrices in 2012 [3], [5]. It has also been reported that different combinations with alginate such as matrigel could be successfully employed for transplanting murine ovarian cells [3]. In 2007, a link was reported for the first time which showed the direct connection between follicle function and biomechanical environment.…”

Section: Introductionmentioning

confidence: 99%

“…In 2017 alginate hydrogels were proposed for transplantation of ovarian follicles considering different grafting times [2]. Alginate is one of the proposed materials which has the minimum grafting time that is reported to be one week [3]. Alginate …”

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confidence: 99%

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A Mechanical Model for an Artificial Oocyte

Hajiyavand¹,

Saadat²,

Stamboulis³

2017

IJMO

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Abstract-Cell injection to introduce a foreign material in biological cells is currently an important area of research. Intervention with biological cells such as an oocyte poses a major limitation towards understanding their biomechanical behaviour, especially during injection, which could result in significant and undesirable deformation of the outer membrane. In this paper, a cross-linked alginate hydrogel is proposed as a material to develop an artificial oocyte to simulate the mechanical properties of a natural human oocyte and use it for mechanical analysis and modelling. A mechanical model and finite element analysis are first presented. The relationship between the injection force and the mechanical properties of the gel are then studied and presented. To validate the mechanical model, experiments were performed using different diameters of an oocyte made using the proposed hydrogel, showing that the modelling results satisfy the experimental demands and that this model can be used to estimate the mechanical properties of other gels too.Index Terms-Artificial oocyte, alginate, hydrogel, finite element model, deformation. I. INTRODUCTIONMany researchers have focused on micro-injection technology which has been proved to be an effective technique introducing foreign materials into cells. This technology can be applied for gene injection, In-vitro fertilisation (IVF), intra-cytoplasmic sperm injection (ICSI), drug development and other biomedical areas notably in cancer treatments [1]. To the best knowledge of the authors, this is the first report of proposing a material for fabricating an artificial oocyte.Accessing human biological oocyte samples for experimental work is very limited, and therefore having suitable artificial samples that could mimic the natural tissues would help to develop appropriate experimental methodology.Synthetic and natural polymers could be considered for the development of artificial tissues. In 2017 alginate hydrogels were proposed for transplantation of ovarian follicles considering different grafting times [2]. Alginate is one of the proposed materials which has the minimum grafting time that is reported to be one week [3]. Alginate

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Section: Model Presentationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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A Mechanical Model for an Artificial Oocyte

Hajiyavand¹,

Saadat²,

Stamboulis³

2017

IJMO

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“…After this period of transplantation, they observed survival and proliferation of ovarian cells and formation of capillaries inside the remaining alginate-matrigel matrix. However, since matrigel cannot be used in a clinical setting, Vanacker et al [19] replaced their alginate-matrigel matrix with a good manufacturing practice (GMP)-produced alginate (NovaMatrix 1 % SLM). They repeated the same experimental design, but also added isolated murine preantral follicles and grafted the matrix inside a peritoneal pocket (Fig.…”

Section: Alginatementioning

confidence: 99%

Artificial Ovary

Amorim¹

2016

Gonadal Tissue Cryopreservation in Fertility Preservation

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Survival rates of many malignant diseases are steadily improving, but for patients of childbearing age, fertility restoration often becomes a vital concern after disease remission. In women, treatments such as chemo/radiotherapy can be very harmful to the ovaries, causing loss of both endocrine and reproductive functions. When gonadotoxic treatment cannot be delayed, ovarian tissue cryobanking is the only way of preserving fertility. However, this technique is not advisable for patients with certain types of cancer, since there is a risk of reintroducing malignant cells present in the cryopreserved tissue. For these patients, a safer alternative could be transplantation of isolated preantral follicles back to their natural environment. To encapsulate and protect isolated follicles, a transplantable artificial ovary needs to be created. The main goal of the artificial ovary is to mimic the natural organ, and for this, it should be composed of a matrix that encapsulates and protects not only the isolated follicles but also autologous ovarian cells and bioactive factors, which are necessary for follicle survival and development. The aim of this chapter is to describe this new technology, its indications, advantages, and the different approaches to create it.

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Retrievable hydrogels for ovarian follicle transplantation and oocyte collection

Rios

Kniazeva

Lee

et al. 2018

Biotech & Bioengineering

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Cancer survivorship rates have drastically increased due to improved efficacy of oncologic treatments. Consequently, clinical concerns have shifted from solely focusing on survival to quality of life, with fertility preservation as an important consideration. Among fertility preservation strategies for female patients, ovarian tissue cryopreservation and subsequent reimplantation has been the only clinical option available to cancer survivors with cryopreserved tissue. However, follicle atresia after transplantation and risk of reintroducing malignant cells have prevented this procedure from becoming widely adopted in clinics. Herein, we investigated the encapsulation of ovarian follicles in alginate hydrogels that isolate the graft from the host, yet allows for maturation after transplantation at a heterotopic (i.e., subcutaneous) site, a process we termed in vivo follicle maturation. Survival of multiple follicle populations was confirmed via histology, with the notable development of the antral follicles. Collected oocytes (63%) exhibited polar body extrusion and were fertilized by intracytoplasmic sperm injection and standard in vitro fertilization procedures. Successfully fertilized oocytes developed to the pronucleus (14%), two-cell (36%), and four-cell (7%) stages. Furthermore, ovarian follicles cotransplanted with metastatic breast cancer cells within the hydrogels allowed for retrieval of the follicles, and no mice developed tumors after removal of the implant, confirming that the hydrogel prevented seeding of disease within the host. Collectively, these findings demonstrate a viable option for safe use of potentially cancer-laden ovarian donor tissue for in vivo follicle maturation within a retrievable hydrogel and subsequent oocyte collection. Ultimately, this technology may provide novel options to preserve fertility for young female patients with cancer.

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First Transplantation of Isolated Murine Follicles in Alginate

Abstract: Our results suggest that an artificial ovary is now conceivable, opening new perspectives to restore fertility in women.

Cited by 62 publications

References 46 publications

A Mechanical Model for an Artificial Oocyte

A Mechanical Model for an Artificial Oocyte

Artificial Ovary

Retrievable hydrogels for ovarian follicle transplantation and oocyte collection

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