First trimester Down syndrome screening with dried blood spots using a dual analyte free beta hCG and PAPP‐A immunofluorometric assay

Abstract

Analysis of free beta hCG and PAPP-A using a dual analyte dried blood spot assay is an effective tool in Down syndrome screening, adding an important option for those considering implementation or modification of existing prenatal screening programs.

Evaluation of a Dried Blood Spot Assay to Measure Prenatal Screening Markers Pregnancy-Associated Plasma Protein A and Free β-Subunit of Human Chorionic Gonadotropin

“…The use of DBSs is routine in the newborn screening of conditions including phenylketonuria, hypothyroidism, and cystic fibrosis (10 ). A firsttrimester aneuploidy screening program that uses a PAPP-A and free-hCG␤ DBS dual analyte method produced by NTD Labs (a PerkinElmer company) has been in clinical use for Ͼ15 years (11 ) and has recently been transferred to the AutoDELFIA (PerkinElmer) (12 ). Furthermore, once the blood spots have dried, free hCG␤ concentrations in DBSs do not appear to increase at room temperature and above (13 ).…”

First trimester prediction of maternal glycemic status

“…Peripheral blood samples were obtained and transmitted on a filter paper to the determining laboratory (NTD Labs, Perkin Elmer, Melville, NY, USA) for analysis. Absolute measurements of pregnancy-associated plasma protein-A, free β-human chorionic gonadotropin, placental growth factor, and alpha-fetoprotein were converted to multiples of the median utilizing reference ranges of a population with documented normal outcome [13].…”

Incorporating first trimester analytes to predict delivery of a large for gestational infant in women with impaired glucose tolerance

“…Peripheral blood samples were obtained and transmitted on filter paper to the determining laboratory (NTD Labs, PerkinElmer, Melville, NY, USA) for analysis. Absolute measurements of pregnancy-associated plasma protein-A (PAPP-A), free β-human chorionic gonadotropin (free β-hCG), placental growth factor (PlGF) and alpha-fetoprotein (αFP) were converted to multiples of the median (MoM) utilizing reference ranges of a population with documented normal outcome [13].…”