FIS1 encodes a GA2-oxidase that regulates fruit firmness in tomato

Ren, Li; Sun, Shuai; Wang, Haijing; Wang, Ketao; Yu, Hong; Zhou, Zhen; Xin, Peiyong; Chu, Jinfang; Zhao, Tongmin; Wang, Huanzhong; Li, Jiayang; Cui, Xia

doi:10.1038/s41467-020-19705-w

Cited by 83 publications

(58 citation statements)

References 57 publications

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“…Our results suggest that SlBES1, as an upstream TF that directly regulates PMEU1 and associated pectin de-methylesterification, could be a powerful target to be explored to extend shelf life without defect in other quality traits to tomato fruits (Figure S4D). A previous study suggested that CRISPR-editing of a hormone biosynthetic gene generated firmer fruit without unfavorable changes in quality (Li et al, 2020). Considering that diverse phytohormone signaling components generally play essential functions via subtle control, such as transcriptional regulation on the target genes (Jiang et al, 2019), it might be potential to achieve improved agronomical traits of crops by genome editing of core signaling components of phytohormones.…”

Section: Slbes1-mediated Direct Transcriptional Inhibition Of Pmeu1 Confers To Fruit Softeningmentioning

confidence: 99%

SlBES1 promotes tomato fruit softening through transcriptional inhibition of PMEU1

Liu

Liang

et al. 2021

iScience

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Section: Slbes1-mediated Direct Transcriptional Inhibition Of Pmeu1 Confers To Fruit Softeningmentioning

confidence: 99%

SlBES1 promotes tomato fruit softening through transcriptional inhibition of PMEU1

Liu

Liang

et al. 2021

iScience

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“…Plant height, mainly confined by stem elongation, is not only a decisive factor that affects plant architecture but also an important agronomic trait that contributes to crop yield ( Wang and Li, 2008 ). Gibberellins (GAs) are a large family of tetracyclic diterpenoid plant hormones that play important roles in multiple plant growth and developmental progresses, including promoting seed germination, stem elongation, flowering, pollen development, as well as fruit growth and firmness ( Hedden and Sponsel, 2015 ; Li et al, 2020 ). The stem elongation function of GA contributed to the Green Revolution in which mutations in GA biosynthesis or signaling are the basis for semidwarf rice and wheat, respectively ( Peng et al, 1999 ; Sasaki et al, 2002 ).…”

Section: Introductionmentioning

confidence: 99%

The GA 20-Oxidase Encoding Gene MSD1 Controls the Main Stem Elongation in Medicago truncatula

Yin

et al. 2021

Front. Plant Sci.

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Plant height is an important agronomic trait that is closely related to biomass yield and crop production. Despite legumes comprise one of the largest monophyletic families that are second only to grasses in terms of economic and nutritional values, due to an ancient genome duplication event, most legume plants have complex genomes, thus the molecular mechanisms that determine plant height are less known in legumes. Here, we report the identification and characterization of MAIN STEM DWARF1 (MSD1), which is required for the plant height in the model legume Medicago truncatula. Loss of function of MSD1 leads to severely reduced main stem height but normal lateral branch elongation in M. truncatula. Histological analysis revealed that the msd1-1 main stem has shorter internodes with reduced cell size and number compared with the wild type, indicating that MSD1 affects cell elongation and cell proliferation. MSD1 encodes a putative GA 20-oxidase that is expressed at significantly higher levels in the main shoot apex than in the lateral shoot apices, suggesting that MSD1 expression is associated with its effect on the main stem elongation. UPLC-MS/MS analysis showed that GA9 and GA4, two identified products of the GA 20-oxidase, were severely reduced in msd1-1, and the dwarf phenotype of msd1-1 could be rescued by supplementation with gibberellic acid GA3, confirming that MSD1 functions as a biologically active GA 20-oxidase. Moreover, we found that disruption of either MtGA20ox7 or MtGA20ox8, homologs of MSD1, has little effects on the elongation of the main stem, while the msd1-1 mtga20ox7-1 mtga20ox8 triple mutants exhibits a severe short main shoot and lateral branches, as well as reduced leaf size, suggesting that MSD1 and its homologs MtGA20ox7 and MtGA20ox8, redundantly regulate M. truncatula shoot elongation and leaf development. Taken together, our findings demonstrate the molecular mechanism of MSD1-mediated regulation of main stem elongation in M. truncatula and provide insights into understanding the functional diversity of GA 20-oxidases in optimizing plant architecture in legumes.

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“…During the process of tomato domestication, particular alleles or genes linked either to environmental stress or to desirable traits may have been gained or lost from the wild ancestors 31 – 34 . For example, the FIS1 locus that contributes to fruit firmness was positively selected during tomato domestication 35 . On the other hand, the SlHAK20 locus, which contributes to the transport of both Na + and K + during salt stress, was lost during domestication 36 .…”

Section: Discussionmentioning

confidence: 99%

Interactions between ShPP2-1, an F-box family gene, and ACR11A regulate cold tolerance of tomato

et al. 2021

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There is a critical need to identify germplasm resources and genes that promote cold tolerance of tomato because global tomato production is threatened by cold stress. We found that the expression of an F-box gene family member named ShPP2-1 from Solanum habrochaites is cold inducible and studied its contribution to cold tolerance. Overexpression of ShPP2-1 in cultivated tomato (AC) reduced cold tolerance by intensifying damage to cell membranes. To explore the underlying molecular mechanism, we conducted a yeast two-hybrid library screen and found that a protein containing ACT domain repeats named ACR11A interacts with PP2-1. Overexpression of SlACR11A in AC enhanced the cold tolerance of seedlings and germinating seeds. Cold tolerance decreased in tomato plants that overexpressed both of these genes. Additionally, we performed seed germination experiments in the cold with 177 tomato accessions and identified two alleles of SlACR11A that differ in one single-nucleotide polymorphism. We found that one of these alleles, SlACR11AG, is significantly enriched in cold-tolerant tomato plants. Taken together, our findings indicate that the combination of low expression levels of PP2-1 and high expression levels of ACR11A can promote cold tolerance. These genes may therefore serve as direct targets for both genetic engineering and improvement projects that aim to enhance the cold tolerance of tomato.

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FIS1 encodes a GA2-oxidase that regulates fruit firmness in tomato

Cited by 83 publications

References 57 publications

SlBES1 promotes tomato fruit softening through transcriptional inhibition of PMEU1

SlBES1 promotes tomato fruit softening through transcriptional inhibition of PMEU1

The GA 20-Oxidase Encoding Gene MSD1 Controls the Main Stem Elongation in Medicago truncatula

Interactions between ShPP2-1, an F-box family gene, and ACR11A regulate cold tolerance of tomato

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