Fisetin Suppresses the Inflammatory Response and Oxidative Stress in Bronchial Epithelial Cells

Abstract

Fisetin is isolated from many fruits and vegetables and has been confirmed to improve airway hyperresponsiveness in asthmatic mice. However, whether fisetin reduces inflammatory response and oxidative stress in bronchial epithelial cells is unclear. Here, BEAS-2B human bronchial epithelial cells were treated with various concentrations of fisetin and then stimulated with tumor necrosis factor-α (TNF-α) or TNF-α/interleukin-4. In addition, ovalbumin-sensitized mice were treated with fisetin to detect inflammatory mediators and oxidative stress expression. Fisetin significantly reduced the levels of inflammatory cytokines and chemokines in TNF-α-stimulated BEAS-2B cells. Fisetin also attenuated intercellular adhesion molecule-1 expression in TNF-α-stimulated BEAS-2B cells, suppressing THP-1 monocyte adhesion. Furthermore, fisetin significantly suppressed airway hyperresponsiveness in the lungs and decreased eosinophil numbers in the bronchoalveolar lavage fluid of asthmatic mice. Fisetin decreased cyclooxygenase-2 expression, promoted glutathione levels, and decreased malondialdehyde levels in the lungs of asthmatic mice. Our findings indicate that fisetin is a potential immunomodulator that can improve the pathological features of asthma by decreasing oxidative stress and inflammation.

The neuroprotective effects of fisetin, a natural flavonoid in neurodegenerative diseases: Focus on the role of oxidative stress

“…Fisetin, chemically 3,7,3′,4′-tetrahydroxyflavone or 7,3′,4′-flavon-3-ol is a polyhydroxy flavonoid with various pharmacological potentials like antioxidant ( Khan et al, 2013 ), anti-inflammatory ( Wu et al, 2022 ), anti-cancer ( Lin et al, 2016 ), neuroprotective ( Kumar et al, 2020 ) and is used in vascular dementia ( Cordaro et al, 2022 ). It occurs in numerous fruits and vegetables, such as grapes, apples, onions, strawberries, and cucumbers ( Cordaro et al, 2022 ).…”

Gypenoside A from Gynostemma pentaphyllum Attenuates Airway Inflammation and Th2 Cell Activities in a Murine Asthma Model

“…At 40 mg/mL methacholine, asthmatic mice treated with gypenoside A or prednisolone had significantly reduced Penh values when compared with the OVA group mice ( Figure 2 A). Mice were anesthetized and bronchoalveolar lavage fluid (BALF) was collected as described previously [ 16 ]. Compared with the OVA group mice, asthmatic mice treated with prednisolone or gypenoside A also had significantly decreased numbers of eosinophils in BALF ( Figure 2 B).…”

Fisetin, a dietary flavonoid, increases the sensitivity of chemoresistant head and neck carcinoma cells to cisplatin possibly through HSP90AA1/IL‐17 pathway

“…In this study, the drug concentrations in animal experiments were selected with reference to the published similar literature (Shanmugam et al, 2018; Wu et al, 2022). Also, the concentration of fisetin was selected by referring to 1/10th of the LD50 concentration in the literature (Hassan et al, 2022).…”

“…The dose of DDP was chosen with reference to similar published literature (Gouttia et al, 2022; H. Li, Huang, Yu, & Lou, 2020), while the dose of fisetin was chosen with reference to the LD50 (median lethal dose) value of fisetin by intraperitoneal injection in mice (250 mg/kg; Hassan et al, 2022), in addition to the doses chosen in other in vivo experiments with fisetin (Shanmugam, Ravindran, Kurian, & Rajesh, 2018; Wu et al, 2022), and was chosen not to exceed 1/10 of the LD50 value.…”