FISH analysis of Lactobacillus biofilms in the gastrointestinal tract of different hosts

Lebeer, Sarah; Verhoeven, Tine; Claes, Ingmar; Hertogh, G. De; Vermeire, Séverine; Buyse, J.; Immerseel, Filip Van; Vanderleyden, Jos; Keersmaecker, Sigrid C. J. De

doi:10.1111/j.1472-765x.2010.02994.x

Cited by 45 publications

(25 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Prior to FISH analysis, serial paraffin sections of 7 m thickness were placed on positively charged slides (SuperFrost Plus, Braunschweig, Germany). FISH was performed as described by Lebeer et al (2011). Enzymatic mixture and hybridization buffer were used according to Czerwiń ski et al (2012).…”

Section: Fluorescence In Situ Hybridization (Fish) Analysismentioning

confidence: 99%

Analysis of biofilm formation by intestinal lactobacilli

et al. 2015

View full text Add to dashboard Cite

In this study, the biofilm-forming potential of intestinal Lactobacillus reuteri strains under different culture conditions was characterized by microtiter plate biofilm assays. Moreover, the spatial organization of exogenously applied L. reuteri L2/6 (a pig isolate) at specific locations in gastrointestinal tract of monoassociated mice was investigated by fluorescence in situ hybridization. We did not detect biofilm formation by tested strains in nutrient-rich de Man-Rogosa-Sharpe (MRS) medium. On the contrary, a highly positive biofilm formation was observed in medium with lower accessibility to the carbon sources and lack of salts. The results obtained confirmed the significant role of Tween 80 and the quantity and nature of the sugars in the growth medium in biofilm formation. The omission of Tween 80 in MRS medium favored the formation of biofilm. Abundant biofilm formation was detected in the presence of lactose, galactose, and glucose. However, a gradual increase in sugar concentration triggered a significant decrease in biofilm formation. In addition, conditions related to the gastrointestinal environment, such as low pH and the presence of bile and mucins, highly modulated biofilm production. This effect seems to be dependent on the specificity and properties of the medium used for cultivation. From the evidence provided by this study we conclude that the biofilm formation capacity of L. reuteri is strongly dependent on the environmental factors and culture medium used.

show abstract

Section: Fluorescence In Situ Hybridization (Fish) Analysismentioning

confidence: 99%

Analysis of biofilm formation by intestinal lactobacilli

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Endogenous Lactobacilli have been demonstrated to exist in biofilm-like communities in the avian and murine guts, while exogenously applied Lactobacillus spp. had difficulty establishing themselves [15]. This work suggests that factors that enhance biofilm formation and adhesion will be important both for the establishment of the native flora and for potential probiotics, to improve their ability to compete and if possible colonise.…”

Section: Introductionmentioning

confidence: 98%

Spontaneous Mutation Reveals Influence of Exopolysaccharide on Lactobacillus johnsonii Surface Characteristics

et al. 2013

View full text Add to dashboard Cite

As a competitive exclusion agent, Lactobacillus johnsonii FI9785 has been shown to prevent the colonization of selected pathogenic bacteria from the chicken gastrointestinal tract. During growth of the bacterium a rare but consistent emergence of an altered phenotype was noted, generating smooth colonies in contrast to the wild type rough form. A smooth colony variant was isolated and two-dimensional gel analysis of both strains revealed a protein spot with different migration properties in the two phenotypes. The spot in both gels was identified as a putative tyrosine kinase (EpsC), associated with a predicted exopolysaccharide gene cluster. Sequencing of the epsC gene from the smooth mutant revealed a single substitution (G to A) in the coding strand, resulting in the amino acid change D88N in the corresponding gene product. A native plasmid of L. johnsonii was engineered to produce a novel vector for constitutive expression and this was used to demonstrate that expression of the wild type epsC gene in the smooth mutant produced a reversion to the rough colony phenotype. Both the mutant and epsC complemented strains had increased levels of exopolysaccharides compared to the wild type strain, indicating that the rough phenotype is not solely associated with the quantity of exopolysaccharide. Another gene in the cluster, epsE, that encoded a putative undecaprenyl-phosphate galactosephosphotransferase, was deleted in order to investigate its role in exopolysaccharide biosynthesis. The ΔepsE strain exhibited a large increase in cell aggregation and a reduction in exopolysaccharide content, while plasmid complementation of epsE restored the wild type phenotype. Flow cytometry showed that the wild type and derivative strains exhibited clear differences in their adhesive ability to HT29 monolayers in tissue culture, demonstrating an impact of EPS on surface properties and bacteria-host interactions.

show abstract

“…The mucin proteins are heavily O-glycosylated and these glycans can serve as adhesion sites for commensal bacteria (118–123). Several studies have shown that intestinal communities can be visualized as biofilms of sessile microorganisms within the mucus layer (116, 124–126). High bacterial cell density within biofilms favors a mode of communication using diffusible small molecules as a mechanism for regulating biofilm formation and for social interactions (97, 127).…”

Section: Signaling Compoundsmentioning

confidence: 99%

Biochemical Features of Beneficial Microbes: Foundations for Therapeutic Microbiology

Engevik

Versalovic

2017

Microbiol Spectr

View full text Add to dashboard Cite

Commensal and beneficial microbes secrete myriad products which target the mammalian host and other microbes. These secreted substances aid in bacterial niche development, and select compounds beneficially modulate the host and promote health. Microbes produce unique compounds which can serve as signaling factors to the host, such as biogenic amine neuromodulators, or quorum-sensing molecules to facilitate inter-bacterial communication. Bacterial metabolites can also participate in functional enhancement of host metabolic capabilities, immunoregulation, and improvement of intestinal barrier function. Secreted products such as lactic acid, hydrogen peroxide, bacteriocins, and bacteriocin-like substances can also target the microbiome. Microbes differ greatly in their metabolic potential and subsequent host effects. As a result, knowledge about microbial metabolites will facilitate selection of next-generation probiotics and therapeutic compounds derived from the mammalian microbiome. In this article we describe prominent examples of microbial metabolites and their effects on microbial communities and the mammalian host.

show abstract

FISH analysis of Lactobacillus biofilms in the gastrointestinal tract of different hosts

Cited by 45 publications

References 27 publications

Analysis of biofilm formation by intestinal lactobacilli

Analysis of biofilm formation by intestinal lactobacilli

Spontaneous Mutation Reveals Influence of Exopolysaccharide on Lactobacillus johnsonii Surface Characteristics

Biochemical Features of Beneficial Microbes: Foundations for Therapeutic Microbiology

Contact Info

Product

Resources

About