2015
DOI: 10.1007/s00299-015-1828-3
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FISH and GISH: molecular cytogenetic tools and their applications in ornamental plants

Abstract: The innovations in chromosome engineering have improved the efficiency of interrogation breeding, and the identification and transfer of resistance genes from alien to native species. Recent advances in molecular biology and cytogenetics have brought revolutionary, conceptual developments in mitosis and meiosis research, chromosome structure and manipulation, gene expression and regulation, and gene silencing. Cytogenetic studies offer integrative tools for imaging, genetics, epigenetics, and cytological infor… Show more

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Cited by 53 publications
(20 citation statements)
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“…The results we obtained with the ISSR and SSR markers confirm the natural hybridization of P. burkartii . However, the various molecular tools available, such as fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) [ 36 , 37 ], could contribute to verify gene or chromosome segment introgression.…”
Section: Discussionmentioning
confidence: 99%
“…The results we obtained with the ISSR and SSR markers confirm the natural hybridization of P. burkartii . However, the various molecular tools available, such as fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) [ 36 , 37 ], could contribute to verify gene or chromosome segment introgression.…”
Section: Discussionmentioning
confidence: 99%
“…The D-type and F-type are common in the majority of Citrus chromosomes, whereas the A, B, C, E, and G types are relatively rare and therefore more meaningful for karyotyping [ 7 , 8 ]. In situ hybridization techniques, such as fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH), the most reliable methods for cytogenetic studies, are very powerful for identifying chromosome characterization and genome organization [ 9 ]. Invariable and heterozygotic karyotype among sweet orange cultivars were revealed by CMA/DAPI staining and in situ hybridization and the putative hybrid origins of these species from mandarin ( C .…”
Section: Introductionmentioning
confidence: 99%
“…In the process of distant hybridization, it is necessary to confirm the genomic composition and chromosome recombination of the progenies. Cytological identification, in situ hybridization (ISH) and molecular markers are the most common methods used to analyze genomic composition and meiotic behavior in distant hybrids (Kerlan et al 1993 ; Li et al 1995 ; Snowdon et al 1997 ; Snowdon 2007 ; Heneen et al 2012 ; Wen et al 2012 ; Younis et al 2015 ; Yu et al 2015 ). However, due to the small genome size and high homology between the A/C genome and A/C subgenome, cytological identification and ISH have many limitations in application (Xiong and Pires 2011 ).…”
Section: Discussionmentioning
confidence: 99%