Data from Lake Ontario tributaries were used to evaluate the efficacy of single-pass backpack electric fishing for stream fish monitoring by: testing the relationship between single-pass catch-per-unit-effort (CPUE) and multiple-pass-based population estimates; comparing species richness estimates derived from single-pass and multiple-pass data and assessing the concordance of fish assemblage patterns described using single-pass and multiple-pass data. Significant correlations were calculated between single-pass CPUE and removal-based population estimates for total catch, 15 species, six taxonomic families, five feeding and four reproductive guilds and tolerant/intolerant species. Strong correlations were more commonly associated with the abundance of individual species than other metrics. Capture probability was not affected by stream size or habitat complexity for most measures. Species accumulation curves and significant correlations (r 2 = 0.9) between single-pass and multiplepass electric fishing indicate that single-pass surveys provide a representative index of species diversity. In addition, within and among-site variation in fish community composition based on single-pass and multiple-pass data were similar.K E Y W O R D S : assessment, backpack electric fishing, fish community, sampling, stream.