Fish Authentication

Carrera, Mónica; Cañas, Benito; Gallardo, José Manuel

doi:10.1007/978-1-4614-5626-1_12

Cited by 8 publications

(5 citation statements)

References 65 publications

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“…Because of the magnitude of the problem, different methods have been implemented to successfully identify the origin of the sample. In recent years, DNA-based methods have increasingly been implemented for authentication because of their high discriminative power. , However, DNA-based procedures are challenged when working with processed products from which it can be difficult to extract DNA in a standardized matter. , This means that alternative methods need to be available and developed to ensure that the correct species can be assigned from samples regardless of their status of processing.…”

Section: Introductionmentioning

confidence: 99%

Authentication of Fish Products by Large-Scale Comparison of Tandem Mass Spectra

et al. 2013

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Authentication of food is a major concern worldwide to ensure that food products are correctly labeled in terms of which animals are actually processed for consumption. Normally authentication is based on species recognition by comparison of selected sequences of DNA or protein. We here present a new robust, proteome-wide tandem mass spectrometry method for species recognition and food product authentication. The method does not use or require any genome sequences or selection of tandem mass spectra but uses all acquired data. The experimental steps were performed in a simple, standardized workflow including protein extraction, digestion, and data analysis. First, a set of reference spectral libraries was generated using unprocessed muscle tissue from 22 different fish species. Query tandem mass spectrometry data sets from "unknown" fresh muscle tissue samples were then searched against the reference libraries. The number of matching spectra could unambiguously identify the origin of all fresh samples. A number of processed samples were also analyzed to further test the robustness and applicability of the method. The results clearly show that the method is also able to correctly identify heavily processed samples.

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Section: Introductionmentioning

confidence: 99%

Authentication of Fish Products by Large-Scale Comparison of Tandem Mass Spectra

et al. 2013

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“…Multitude of biological and chemical methods have been developed and successfully used for authentication and traceability of seafood. Molecular strategies such as DNA barcoding (including mitochondrial-DNA), PCR-based screening and targeted proteomics such as MALDI-TOF focus on evaluation of organic compounds for accurate seafood authentication ( Asensio Gil, 2007 ; Carrera et al, 2013 ; Mazzeo et al, 2008 ; Mazzeo and Siciliano, 2016 ). Inorganic fractions in fish are also used for traceability with geochemical profile (in terms of major, trace and ultra-trace elements) and stable isotope ratios ( Dempson and Power, 2004 ; Molkentin et al, 2007 ; Varrà et al, 2021b ).…”

Section: Introductionmentioning

confidence: 99%

What the fish? Tracing the geographical origin of fish using NIR spectroscopy

Dalal,

Ofano,

Ruggiero

et al. 2024

Current Research in Food Science

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“…For this purpose, protein and DNA molecular markers have been developed. 3 DNA can still be analyzed even if the product has undergone intensive processing steps and is hardly influenced by exogenous factors. In addition, DNA has a high identification power at the species and variety levels.…”

Section: ■ Introductionmentioning

confidence: 99%

“…However, analysis of morphological traits is possible only to a limited extent for processed fish products if head, fins, and skin get removed. For this purpose, protein and DNA molecular markers have been developed . DNA can still be analyzed even if the product has undergone intensive processing steps and is hardly influenced by exogenous factors.…”

Section: Introductionmentioning

confidence: 99%

Fast and User-Friendly Detection of Flatfish Species (Pleuronectes platessa and Solea solea) via Loop-Mediated Isothermal Amplification (LAMP)

Wax,

Pförtner,

Holz

et al. 2023

J. Agric. Food Chem.

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The detection of a Cytochrome b gene (cytb) for species differentiation in fish is intensively used. A fast alternative to expensive and time-consuming DNA barcoding is loop-mediated isothermal amplification (LAMP) in combination with efficient readout systems. For this reason, we developed LAMP assays for rapid species detection of Pleuronectes platessa and Solea solea, two economically important flatfish species in Europe that are prone to mislabeling. Species-specific primer sets targeting cytb were designed, and LAMP assays were optimized. With the optimized LAMP assays, we were able to detect up to 0.1 and 0.01 ng of target DNA of P. platessa and S. solea, respectively, and in each case up to 1% (w/w) of target species in mixtures with nontarget species. For future on-site detection, a lateral flow assay and a pocket-sized lab-on-phone assay were used as readout systems. The lab-on-phone assay with the S. solea specific primer set revealed cross-reactivity to Solea senegalensis. The assay targeting P. platessa proved to be highly specific. Both assays could be performed within 45 min and provided rapid and easy detection of fish species.

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Fish Authentication

Cited by 8 publications

References 65 publications

Authentication of Fish Products by Large-Scale Comparison of Tandem Mass Spectra

Authentication of Fish Products by Large-Scale Comparison of Tandem Mass Spectra

What the fish? Tracing the geographical origin of fish using NIR spectroscopy

Fast and User-Friendly Detection of Flatfish Species (Pleuronectes platessa and Solea solea) via Loop-Mediated Isothermal Amplification (LAMP)

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