Larvae and juveniles of bully sleepers are the primary targets for the local community surrounding Cisolok River, and this traditional fishing activity is called nyalawean. The present study aims to uncover fish larval diversity and recruitment of bully sleepers in the Cisolok river estuary. Fish sampling using a lift net was conveyed monthly from December 2020 to February 2021. Fish specimens were identified to the lowest taxon through a morphological approach and DNA Barcoding. Catch per unit effort measured the abundance of fish larvae and juveniles (CPUE, Ind.h−1). Only one species was found during the study period, namely Bunaka gyrinoides. A total of 7,353 larvae and juveniles of B. gyrinoides were captured, consisting of flexion (45 individuals; 4-5.9 mm BL), postflexion (6,887; 6-11 mm BL), and early juvenile (441; 11.1-14.8 mm BL). Postflexion larvae were the most abundant specimens, contributing 88.4-95.5%; 96-81.1%, 94.2-97.8% to the total catches in December, January, and February. Larvae and juveniles were more abundant during daylight (671-1591 Ind.h-1) rather than nighttime (147-567 Ind.h-1). These findings indicate that bully sleepers recruit and migrate upstream into the freshwater area of the Cisolok River during the day and nighttime synchronized with the tidal fluxes.