Fish Cell Line As an In Vitro Test System for Analyzing Chromosome Aberrations

Oh, Yeon-Kyung; Lee, J.-S.; Park, E.-H.

doi:10.1007/s001280084

Cited by 4 publications

(3 citation statements)

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“…In vitro culture of fish cells provides an important tool for studying cellular physiology, molecular biology, functional genomics, toxicology and transgenic applications (Hightower and Renfro, 1988;Bejar et al, 2005;Bahich and Borenfreund, 1991;Gagné and Blaise, 2000;Conception et al, 2001;Oh et al, 2001;Tiago and Laizé, 2008;Ma et al, 2001;Pombinho et al, 2004). By 1994, more than 150 cell lines have been established from teleost fishes, while relatively few cell lines were developed in marine fishes (Fryer and Lannan, 1994).…”

Section: Introductionmentioning

confidence: 97%

Establishment and characterization of a new cell line from the kidney of spotted halibut Verasper variegates

Wang

Chen

Sha

et al. 2010

J. Ocean Univ. China

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A cell line, SHK, was derived from the kidney of spotted halibut Verasper variegates. The cell line was subcultured more than 40 passages in minimum essential medium (MEM) supplemented with fetal bovine serum (FBS) and 10 ng ml -1 basic fibroblast growth factor (bFGF). Cell morphology from primary culture and subculture was observed continuously by microscopy. The SHK cell line consisted predominantly of fibroblast-like cells. The cell line was able to grow between 20℃ and 30℃ with the optimum growth at 24℃ and with a reduced growth between 12℃ and 20℃. The growth rate of the cells increased as the proportion of FBS increased from 10% to 20% at 28℃ with optimum growth at the concentration of 20%. The doubling time of the cells was determined to be 44.8 h. Chromosome analysis revealed that 52% of the SHK cells maintained a normal diploid chromosome number (2n=46). The cells were successfully transfected with green fluorescent protein (GFP) reporter plasmids and the expression of GFP gene in the cells indicated the possible utility of the cells in gene expression studies. The cells were infected by lymphosystis disease virus (LCDV) and found to be susceptible to the virus in cytopathic effect (CPE) observation. The infection was confirmed by PCR and electron microscopy experiments, which proved the existence of the viral particles in the cytoplasm of the virus-infected cells.

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Section: Introductionmentioning

confidence: 97%

Establishment and characterization of a new cell line from the kidney of spotted halibut Verasper variegates

Wang

Chen

Sha

et al. 2010

J. Ocean Univ. China

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“…As the first continuous teleost fish cell culture (RTG‐2) was developed from rainbow trout [ Oncorhynchus mykiss ; (Walbaum 1972)] gonad tissue more than 40 years ago, up to now, over 283 fish cell lines have been developed (Chen, Sha & Ye ; Chen, Ren, Sha & Shi ; Qin, Wu, Jia, Hegde & Zhang ; Sahul Hameed, Parameswaran, Shukla & Bright ; Yi, Hong & Hong ; Lakra, Swaminathan & Joy ), while only less than 100 cell lines originated from marine fishes (Chen & Qin ). Fish cell lines are generally used for isolating, identifying and studying fish pathogenic viruses; moreover, the majority of fish cell lines are providing important contribution in studies relating to biomedical research, fish immunology (Clem, Ely, Wilson, Chinchar, Stuge, Barker, Luft, Rycyzyn, Hogan, van Lopik & Miller ), virology (Ruiz, Schyth, Encinas, Tafalla, Estepa, Lorenzen & Coll ), toxicology (Papis, Davies & Jha ; Oh, Lee & Park ), physiology (Rode, Berg & GjØen ), carcinogenesis (Salinas, Meseguer & Esteban ), transgenic applications (Fan & Collodi ; Ku, Teng, Wang & Lu ), genetic regulation and expression, DNA replication and repair, etc. (Nicholson, Danner & Wu ; Ormonde, HÖrstedt, O'Toole & Milton ; Smith, Palmer & Houlihan ; Huang, Jou, Ho, Lee, Jeng, Hsieh & Tsai ; Forest, Nishikawa, Kobayashi, Parton, Bayne & Barnes ; Cosnefroy, Brion, Guillet, Laville, Porcher, Balaguer & AÏt‐AÏssa ; Lakra, Swaminathan & Joy ).…”

Section: Introductionmentioning

confidence: 99%

Establishment of a continuous cell line from female half-smooth tongue sole (Cynoglossus semilaevis)

2015

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A new cell line (TSHC) derived from heart tissues was established from female half‐smooth tongue sole (Cynoglossus semilaevis), an economically important marine fish species in China. The cell line had been subcultured for more than 30 times over a period of 200 days. The cell line was optimally maintained at 24°C in minimum essential medium (MEM) medium containing foetal bovine serum (FBS), 2‐mercaptoethanol (2‐Me), sodium pyruvate, basic fibroblast growth factor (bFGF) and antibiotics. The TSHC cells were mostly composed of fibroblast‐like cells. Chromosome analysis revealed that the TSHC cell line had a normal diploid karyotype with 2n = 42, containing the heterogametic W chromosome. The TSHC cell line was susceptible to infection by flounder Lymphocystis disease virus (LCDV). Although an atypical cytopathic effect and only few of virus particles in the cytoplasm was observed, it provides a research material on the cell–pathogen interaction research about the viral infection of non‐host species.

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“…The application of these fish cell lines provided valuable information on fish developmental biology, immunology (Clem et al, 1996), virology (Ruiz et al, 2009), toxicology (Oh et al, 2001), physiology (Rode et al, 1997) and carcinogenesis (Salinas et al, 2008). Traditionally, the primary uses of a cultured cell line were for isolation and multiplication of fish viruses.…”

Section: Introductionmentioning

confidence: 99%

Establishment and characterization of a head kidney cell line from large yellow croaker Pseudosciaena crocea

Wang

Nie

et al. 2014

Journal of Fish Biology

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A continuous cell line, LYCK, derived from the head kidney of large yellow croaker Pseudosciaena crocea was established and characterized. The LYCK cell line multiplied well in Leibovitz's-15 (L-15) medium supplemented with 10% foetal bovine serum (FBS) at 28° C. This cell line, with a population doubling time of 29·5 h at passage 35, has been subcultured for >70 passages. Microscopically, LYCK cells were fibroblast-like. Chromosomal analysis revealed that the modal diploid chromosome number of LYCK cells was 48, which was identical to that of the P. crocea kidney. The cellular fluorescence could be observed in LYCK cells at 48 h after being transfected with pEGFP-N1 plasmid DNA, indicating that LYCK cells were competent for target gene expression in vitro. The expression of mRNA transcripts of the antiviral immune-related molecules interferon regulatory factor-3 I(ir3), interferon regulatory factor-7 (irf7), melanoma differentiation-associated protein 5 (mda5) was obviously up-regulated in LYCK cells in response to the stimulation with poly (I:C), whereas the expression of mRNA transcripts of the inflammatory cytokines tumour necrosis factor-α2 (tnfTNF-α2), interleukin-8 (il8), CC chemokine (lycCC) was up-regulated by lipopolysaccharide. These results indicated that the LYCK cell line could serve as a valuable tool for studies on immune-related gene functions in vitro.

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Fish Cell Line As an In Vitro Test System for Analyzing Chromosome Aberrations

Cited by 4 publications

References 0 publications

Establishment and characterization of a new cell line from the kidney of spotted halibut Verasper variegates

Establishment and characterization of a new cell line from the kidney of spotted halibut Verasper variegates

Establishment of a continuous cell line from female half-smooth tongue sole (Cynoglossus semilaevis)

Establishment and characterization of a head kidney cell line from large yellow croaker Pseudosciaena crocea

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